大黄对瘢痕成纤维细胞增殖抑制作用及其有效成分研究
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摘要
目的探讨大黄有效成分对瘢痕成纤维细胞的增殖作用。方法取中药大黄,分别以水、乙酸乙酯、石油醚、饱和正丁醇、氯仿回流提取,回流提取,得到相应提取物,以此培养人增生性瘢痕成纤维细胞,酶标仪测定增殖抑制率。以甲醇溶解具有较强增殖抑制作用的乙酸乙酯提取物,以高效液色相谱法鉴定高活性成分,同法进行抗增殖作用分析。结果大黄乙酸乙酯提取物HSF增殖抑制率17.83%,抗细胞增殖活性明显高于其他提取物及对照组,其主要活性成分为及对应HSF增殖抑制率分别为没食子酸41.76%、大黄酸36.7%、大黄素38.3%。结论大黄乙酸乙酯提取物含有活性成分没食子酸、大黄酸和大黄素,具有良好的抑制瘢痕成纤维细胞增殖作用,可作为大黄治疗增殖性瘢痕物质理论依据参考。
Objective To investigate the effect of rhubarb active ingredient on the proliferation of scar fibroblasts.Methods The traditional Chinese medicine rhubarb was extracted with water, ethyl acetate, petroleum ether, saturated n-butanol and chloroform, and refluxed to obtain the corresponding extracts. The human hypertrophic scar fibroblasts were cultured and the proliferation inhibition was measured by microplate reader. The ethyl acetate extract with strong proliferation inhibition effect was dissolved in methanol, and the high active ingredient was identified by high performance liquid chromatography. The anti-proliferation effect was analyzed by the same method. Results The inhibition rate of HSF in ethyl acetate extract of Rhubarb was 17.83%. The anti-cell proliferation activity was significantly higher than that of other extracts and control group. The main active ingredients and corresponding HSF proliferation inhibition rates were 41.76% for gallic acid and 36.7% for rhein and emodin 38.3%. Conclusion Ethyl acetate extract of Rhubarb contains active ingredients gallic acid, rhein and emodin, which has a good effect on inhibiting the proliferation of scar fibroblasts. It can be used as a theoretical basis for the treatment of proliferative scars in rhubarb.
Objective To investigate the effect of rhubarb active ingredient on the proliferation of scar fibroblasts.Methods The traditional Chinese medicine rhubarb was extracted with water, ethyl acetate, petroleum ether, saturated n-butanol and chloroform, and refluxed to obtain the corresponding extracts. The human hypertrophic scar fibroblasts were cultured and the proliferation inhibition was measured by microplate reader. The ethyl acetate extract with strong proliferation inhibition effect was dissolved in methanol, and the high active ingredient was identified by high performance liquid chromatography. The anti-proliferation effect was analyzed by the same method. Results The inhibition rate of HSF in ethyl acetate extract of Rhubarb was 17.83%. The anti-cell proliferation activity was significantly higher than that of other extracts and control group. The main active ingredients and corresponding HSF proliferation inhibition rates were 41.76% for gallic acid and 36.7% for rhein and emodin 38.3%. Conclusion Ethyl acetate extract of Rhubarb contains active ingredients gallic acid, rhein and emodin, which has a good effect on inhibiting the proliferation of scar fibroblasts. It can be used as a theoretical basis for the treatment of proliferative scars in rhubarb.
引文
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[9]刘庭波,李秀琴,王文峰.大黄素衍生物E11的筛选及对多发性骨髓瘤细胞增殖抑制和诱导凋亡作用的研究[J].中国实验血液学杂志,2018,26(5):1407-1413.
[2]潘学洪,王晓东.抑制Akt信号通路对病理性瘢痕成纤维细胞生长、细胞周期及凋亡的影响[J].郑州大学学报:医学版,2018,53(2):229-233.
[3]李响,刘宏伟,梁杰,等.异泽兰黄素抑制瘢痕组织成纤维细胞增殖的机制[J].中国老年学杂志,2017,37(22):5495-5498.
[4]刘启胜.病理性瘢痕成纤维细胞中凋亡因子Fas、Bcl-2、survivin、caspase-3蛋白的表达及意义[J].中国美容医学,2017,26(11):63-65,123.
[5]刘理静,尹辉明,贺兼斌,等.大黄素抑制肺成纤维细胞增殖及转分化和胶原蛋白合成[J].细胞与分子免疫学杂志,2016,32(7):921-925.
[6]唐楠,常剑,饶竞.大黄酸通过抑制ERK途径诱导胶质瘤细胞增殖分化的实验研究[J].临床神经外科杂志,2018,15(4):246-249.
[7]刘漪沦,邓峰美,刘卫华,等.大黄素对增生性瘢痕成纤维细胞的作用[J].医药导报,2014,33(12):1566-1570.
[8]杨明,柯友辉,柯晨,等.中医防治增生性瘢痕的基础研究进展[J].中国美容医学,2018,27(1):152-156.
[9]刘庭波,李秀琴,王文峰.大黄素衍生物E11的筛选及对多发性骨髓瘤细胞增殖抑制和诱导凋亡作用的研究[J].中国实验血液学杂志,2018,26(5):1407-1413.