果蝇低温麻醉设备构建及其在实验教学中的应用
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摘要
果蝇是重要的模式生物,果蝇的麻醉操作在遗传学实验过程中具有重要的地位和作用,乙醚是用来麻醉果蝇的经典实验试剂,为了克服乙醚的安全隐患和缺乏环境友好的缺陷,通过长时间反复的探索与改进,设计并构建低温果蝇麻醉设备,该设备在本科实验教学中取得了良好的效果。
Drosophila melanogaster is an important model organism.The anesthetic operation of Drosophila melanogaster plays an important role in genetics experiments and ether is common used for anesthetize. As we all know, ether has certain security risks and is not friendly to the environment. Thus, low temperature anesthesia equipment was designed and constructed, through repeated exploration and improvement over a long period of time. The device has achieved good results in undergraduate experimental teaching.
Drosophila melanogaster is an important model organism.The anesthetic operation of Drosophila melanogaster plays an important role in genetics experiments and ether is common used for anesthetize. As we all know, ether has certain security risks and is not friendly to the environment. Thus, low temperature anesthesia equipment was designed and constructed, through repeated exploration and improvement over a long period of time. The device has achieved good results in undergraduate experimental teaching.
引文
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[3]张春红,张敏菁,焦文娟,等.果蝇衰老的调控机制[J].昆虫学报,2016,59(1):102-110.
[4]霍桂桃,吕建军,屈哲,等.果蝇在肿瘤学研究中的优势及应用前景[J].遗传,2014,36(1):30-40.
[5]韩萌,马萍丽,叶春艳,等.阿尔茨海默病果蝇模型中Aβ毒性的长度依赖性[J].中国老年学杂志,2015,35(5):1315-1317.
[6]van Dijken FR, van Sambeck MJPW, Scharloo W Influence of anaesthesia by carbon dioxide and ether on locomotor activity in Drosophila melanogaster.[J]. Experientia, 1977(33):1360-1361.
[7]周杰,张雪兰,刘艳,等.一种果蝇麻醉装置[P].中国:专利号ZL201420320714.6.2014.
[8]刘艳,孟令军,逯家辉,等.低温麻醉技术在果蝇遗传学实验教学中的应用[J].生命的化学,2016,36(1):126-128.
[9]中华人民共和国公安部令第87号.易制毒化学品购销和运输管理办法[Z].2006-10-01.
[10]滕利荣,孟庆繁.生物学基础实验教程(II):遗传学实验生物、化学实验、分子生物学实验[M].第三版.北京:科学出版社,2008.
[11]周杰,张雪兰,刘艳,等.一种对果蝇实施低温麻醉的方法[P].中国:专利号ZL201410267683.7.
[12]张雪兰,王亚文,侯杰,等.冰盐浴低温处理对果蝇麻醉效应探索[J].生物学通报,2015,50(5):50-51.
[2]袁婺洲,BodmerR,朱传炳,等.利用RNAi技术研究果蝇心脏发育基因的功能[J].遗传学报,2002,29(1):34-38.
[3]张春红,张敏菁,焦文娟,等.果蝇衰老的调控机制[J].昆虫学报,2016,59(1):102-110.
[4]霍桂桃,吕建军,屈哲,等.果蝇在肿瘤学研究中的优势及应用前景[J].遗传,2014,36(1):30-40.
[5]韩萌,马萍丽,叶春艳,等.阿尔茨海默病果蝇模型中Aβ毒性的长度依赖性[J].中国老年学杂志,2015,35(5):1315-1317.
[6]van Dijken FR, van Sambeck MJPW, Scharloo W Influence of anaesthesia by carbon dioxide and ether on locomotor activity in Drosophila melanogaster.[J]. Experientia, 1977(33):1360-1361.
[7]周杰,张雪兰,刘艳,等.一种果蝇麻醉装置[P].中国:专利号ZL201420320714.6.2014.
[8]刘艳,孟令军,逯家辉,等.低温麻醉技术在果蝇遗传学实验教学中的应用[J].生命的化学,2016,36(1):126-128.
[9]中华人民共和国公安部令第87号.易制毒化学品购销和运输管理办法[Z].2006-10-01.
[10]滕利荣,孟庆繁.生物学基础实验教程(II):遗传学实验生物、化学实验、分子生物学实验[M].第三版.北京:科学出版社,2008.
[11]周杰,张雪兰,刘艳,等.一种对果蝇实施低温麻醉的方法[P].中国:专利号ZL201410267683.7.
[12]张雪兰,王亚文,侯杰,等.冰盐浴低温处理对果蝇麻醉效应探索[J].生物学通报,2015,50(5):50-51.