氨水提取联合陶瓷膜超滤技术纯化甘草苷工艺研究
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摘要
目的建立一种适合于工业化生产的甘草苷提取纯化工艺路线。方法以甘草苷的提取率为指标,采用正交试验确定最佳提取条件;并以甘草苷的保留率和除杂率为指标,通过正交试验优选最佳超滤工艺参数。结果最佳提取条件为0.75%氨水24倍,提取3次,每次60 min,甘草苷平均提取率为72.3%。最佳超滤工艺参数:10 nm无机陶瓷膜,压力0.12 MPa,温度25℃,甘草苷平均保留率为98.9%,平均除杂率为23.3%。结论该工艺生产成本低,安全性好,适合工业化应用。
Objective To establish a suitable extraction and purification process line for industrial production of liquiritin. Methods With the extraction rate of liquiritin as index, orthogonal test was used to determine the optimum conditions; with the retention rate of liquiritin and impurity removal rate as the indexes, orthogonal test was used to optimize the best ultrafiltration process parameters. Results The optimum extraction conditions were: 24 times 0.75% ammonia water, extracted three times, each time under 60 min. The liquiritin average extraction rate was 72.3%. The best ultrafiltration process parameters were: 10 nm inorganic ceramic membrane, pressure of 0.12 MPa, temperature of 25 ℃. The liquiritin average retention rate was 98.9%, and the average removal rate of impurity was 23.3%. Conclusion This process has low production cost and good safety, and is suitable for industrial application.
Objective To establish a suitable extraction and purification process line for industrial production of liquiritin. Methods With the extraction rate of liquiritin as index, orthogonal test was used to determine the optimum conditions; with the retention rate of liquiritin and impurity removal rate as the indexes, orthogonal test was used to optimize the best ultrafiltration process parameters. Results The optimum extraction conditions were: 24 times 0.75% ammonia water, extracted three times, each time under 60 min. The liquiritin average extraction rate was 72.3%. The best ultrafiltration process parameters were: 10 nm inorganic ceramic membrane, pressure of 0.12 MPa, temperature of 25 ℃. The liquiritin average retention rate was 98.9%, and the average removal rate of impurity was 23.3%. Conclusion This process has low production cost and good safety, and is suitable for industrial application.
引文
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[11]王芸芸,刘利军.超滤膜技术用于甘草总黄酮的分离纯化[J].化学研究与应用,2012,24(4):646-649.
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[13]魏舒畅.超滤和膜萃取耦合技术纯化几类天然物质的系统及方法:201310047146.7[P].2013-05-01.
[2]杨云,卞广兴,吕秋军.甘草苷对原代海马神经细胞的保护和营养作用[J].中国中药杂志,2008,33(8):931-935.
[3]董晞,赵世萍,刘岩,等.甘草苷对乌头碱致心肌细胞损伤的保护作用[J].中华中医药杂志,2009,24(2):163-166.
[4]肖渊.甘草苷的抗抑郁作用及其机理研究[D].北京:北京中医药大学,2009.
[5]柳春,刘晓霞,魏舒畅,等.基于红芪芒柄花素保留率建立纤维性根茎药材超滤预测模型[J].天然产物研究与开发,2015,27(9):1550-1553.
[6]王继龙,刘晓霞,魏舒畅,等.基于BP神经网络的纤维性根茎药材酶解提取-超滤纯化的临界通量与压力预测[J].天然产物研究与开发,2016,28(4):586-590.
[7]阚微娜,谭天伟.微波法提取甘草中有效成分的研究[J].中草药,2006,37(1):61-64.
[8]徐向君,余金鹏,袁媛,等.闪式提取及高速逆流色谱联用提取高纯度甘草苷[J].中成药,2016,38(1):72-76.
[9]吴伟康,奉建芳,黄小蕊,等.甘草提取工艺的初步研究[J].中草药,2001,32(3):210-212.
[10]古川,梁艳妮,唐志书,等.不同分离纯化技术对甘草水提液组分的影响[J].中国实验方剂学杂志,2015,21(20):5-8.
[11]王芸芸,刘利军.超滤膜技术用于甘草总黄酮的分离纯化[J].化学研究与应用,2012,24(4):646-649.
[12]瞿其扬,李存玉,郑云枫,等.不同溶液体系中甘草酸的超滤分离规律研究[J].中成药,2015,37(12):2775-2778.
[13]魏舒畅.超滤和膜萃取耦合技术纯化几类天然物质的系统及方法:201310047146.7[P].2013-05-01.