基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺
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摘要
目的基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺,为其工业化生产提供参考。方法采用基于G1-熵权法的主客观组合赋权法和L9(34)正交设计,以提取次数、提取时间和加水量为考察因素,毛蕊异黄酮葡萄糖苷、橙皮苷、粗多糖、醇浸出物提取量及出膏率为评价指标,优选黄芪百合颗粒的最佳水提工艺;以多通道管状陶瓷超滤膜的膜孔径、操作压强和滤过温度为考察因素,毛蕊异黄酮葡萄糖苷、橙皮苷、粗多糖、醇浸出物保留率及除杂率为评价指标,优选其最佳超滤纯化工艺。结果黄芪百合颗粒的最佳水提工艺为用总量20倍的水加热回流提取2次,每次提取75 min;最佳超滤工艺为膜孔径50 nm,操作压强0.10 MPa,滤过温度45℃。在此条件下,3批验证试验数据组间均无明显差异。结论优选的提取、纯化工艺稳定可行,可为黄芪百合颗粒的工业化生产提供实验依据。
Objective To determine the optimum extraction and purification technology of Huangqi Baihe Granules(HBG) by using orthogonal design and combination empowerment based on G1-entropy method, so as to provide a reference for the industrial production. Methods With the ethanol extraction amount and paste-forming rate of calycosin7-O-β-D-glucopyranoside, hesperidin, crude polysaccharide as evaluation index, and with the extraction times, extraction time and the water adding amount as investigate factors, then the combination empowerment method based on G1-entropy method and orthogonal design were used to optimize the extraction technology of HBG. The same combined methods were used to optimize the purification technology of HBG with retention rate and removal rate of impurity of ethanol extract of calycosin7-O-β-D-glucopyranoside, hesperidin and crude polysaccharide, as evaluation index, and with the membrane pore diameter, operating pressure and filtration temperature of multi-channel tubular ceramic ultrafiltration membrane as investigate factors. Results The test results showed that the optimum extraction technology was as follows: Extracted 2 times with 20 times the amount of water and each for 75 min. The optimum ultrafiltration technology was as follows: Multi-channel tubular inorganic ceramic membrane at 50 nm, operating pressure at 0.10 MPa, filtration temperature at 45 ℃. Under such condition, there was no significant difference between verification groups of three batches. Conclusion The optimized extraction and purification process is stable and feasible by verification, which can provide experimental basis for industrial production of HBG.
Objective To determine the optimum extraction and purification technology of Huangqi Baihe Granules(HBG) by using orthogonal design and combination empowerment based on G1-entropy method, so as to provide a reference for the industrial production. Methods With the ethanol extraction amount and paste-forming rate of calycosin7-O-β-D-glucopyranoside, hesperidin, crude polysaccharide as evaluation index, and with the extraction times, extraction time and the water adding amount as investigate factors, then the combination empowerment method based on G1-entropy method and orthogonal design were used to optimize the extraction technology of HBG. The same combined methods were used to optimize the purification technology of HBG with retention rate and removal rate of impurity of ethanol extract of calycosin7-O-β-D-glucopyranoside, hesperidin and crude polysaccharide, as evaluation index, and with the membrane pore diameter, operating pressure and filtration temperature of multi-channel tubular ceramic ultrafiltration membrane as investigate factors. Results The test results showed that the optimum extraction technology was as follows: Extracted 2 times with 20 times the amount of water and each for 75 min. The optimum ultrafiltration technology was as follows: Multi-channel tubular inorganic ceramic membrane at 50 nm, operating pressure at 0.10 MPa, filtration temperature at 45 ℃. Under such condition, there was no significant difference between verification groups of three batches. Conclusion The optimized extraction and purification process is stable and feasible by verification, which can provide experimental basis for industrial production of HBG.
引文
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[4]冯博,刘震,邢雁伟,等.传统中(藏)药物防治急性高原病研究进展[J].中国中药杂志,2013,38(12):1876-1880.
[5]蔡钟奇,李泱.急性高原病药物防治临床试验研究进展[J].世界最新医学信息文摘,2017,17(34):28-29.
[6]李连结,姚建刚,龙立波,等.组合赋权法在电能质量模糊综合评价中的应用[J].电力系统自动化,2007,31(4):56-60.
[7]田彦芳,万海同,朱紫烨,等.基于熵权法的多目标筛选甘草黄酮类成分纯化工艺[J].中草药,2016,47(7):1118-1125.
[8]陈端,曹阳,夏辉,等.基于熵权法和G1法的大坝监测指标权重融合[J].水电能源科学,2012,30(6):92-94.
[9]曾江,蔡东阳.基于组合权重的蒙特卡洛电压暂降评估方法[J].电网技术,2016,40(5):1469-1475.
[10]魏舒畅,王继龙,李昶,等.改良差示酚硫法测定红芪粗多糖的方法研究[J].中成药,2013,35(3):634-636.
[11]王继龙,刘晓霞,魏舒畅,等.基于BP神经网络的纤维性根茎药材酶解提取-超滤纯化的临界通量与压力预测[J].天然产物研究与开发,2016,28(4):586-590.
[12]姚美村,齐莹,毕开顺,等.黄芪药效物质基础研究[J].中国野生植物资源,2000,19(2):33-36.
[13]胡敏敏,蔡宝昌,张志杰,等.百合多糖的药效学研究[J].中药新药与临床药理,2007,18(2):107-109.
[14]李林,张志杰,蔡宝昌.中药百合有效部位的药效学筛选[J].南京中医药大学学报,2005,21(3):175-177.
[15]龙怡,李佳川,孟宪丽.红景天有效成分对缺氧缺糖心肌细胞损伤的保护作用研究[J].中药药理与临床,2010,26(1):24-25.
[16]刘娟,王莉.枸杞子、红景天对小鼠耐力影响的研究[J].农业科学研究,2011,32(2):92-94.
[17]朱应怀,刘晓霞,王继龙,等.基于陶瓷膜超滤技术的甘草酸和甘草苷同步提取纯化工艺研究[J].中草药,2016,47(23):4173-4178.