高效液相色谱法同时测定饮料中柠檬黄、日落黄和诱惑红的含量
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摘要
采用高效液相色谱-紫外检测法(HPLC-UV)同时测定了有色饮料中的合成色素柠檬黄(E102)、日落黄(E110)和诱惑红(E129)。样品经溶解、超声去除气泡、稀释、0.45μm的滤膜过滤后,直接用HPLC分析。考察了流动相的体系及比例、紫外测定波长和柱温对3种色素的分离检测效果的影响。确定流动相为V(甲醇)∶V(乙酸铵(0.02mol·L-1))=40∶60,紫外检测波长为254nm,柱温为30℃。结果表明:柠檬黄、日落黄和诱惑红在该条件下均有较好的分离和检测,定量范围分别为0.30～10、0.50～10和1.0～10μg·mL-1,3种色素的线性相关系数R2为0.999 0～0.999 3,检出限为0.09～0.50μg·mL-1,相对标准偏差为0.07%～0.16%,回收率为94.77%～100.23%。该方法样品预处理简单、分离效果好,适用于饮料中柠檬黄、日落黄和诱惑红的同时测定。
In this paper,tartrazine(E102),sunset yellow(E110),and allura red(E129)were determined by high performance liquid chromatography with UV detector(HPLC-UV).The samples were dissolved and degassed by ultrasonication,diluted with water,filtered through0.45μm millipore filters,and analyzed by HPLC.The effects of mobile phase,UV detection wavelength,and temperature on the separation of three pigments were investigated.It was finally determined that the mobile phase was V(methanol)∶V(ammonium acetate(0.02 mol·L-1))= 40∶60,the detection wavelength was 254 nm,and the temperature was 30℃.The results showed that tartrazine,sunset yellow,and allura red were well separated and detected under the above conditions.The limits of quantification of three pigments were 0.30—10,0.50—10 and 1.0—10μg· mL-1,respectively.Correlation coefficients(0.999 0—0.999 3),limits of detection(0.09—0.50μg·mL-1),standard deviations of reproducibility(0.07%—0.16%)and recoveries(94.77%—100.23%)were determined.The method with the properties of simple pretreatment and good effect is suitable for simultaneous quantitative the content of synthetic pigments in beverages.
In this paper,tartrazine(E102),sunset yellow(E110),and allura red(E129)were determined by high performance liquid chromatography with UV detector(HPLC-UV).The samples were dissolved and degassed by ultrasonication,diluted with water,filtered through0.45μm millipore filters,and analyzed by HPLC.The effects of mobile phase,UV detection wavelength,and temperature on the separation of three pigments were investigated.It was finally determined that the mobile phase was V(methanol)∶V(ammonium acetate(0.02 mol·L-1))= 40∶60,the detection wavelength was 254 nm,and the temperature was 30℃.The results showed that tartrazine,sunset yellow,and allura red were well separated and detected under the above conditions.The limits of quantification of three pigments were 0.30—10,0.50—10 and 1.0—10μg· mL-1,respectively.Correlation coefficients(0.999 0—0.999 3),limits of detection(0.09—0.50μg·mL-1),standard deviations of reproducibility(0.07%—0.16%)and recoveries(94.77%—100.23%)were determined.The method with the properties of simple pretreatment and good effect is suitable for simultaneous quantitative the content of synthetic pigments in beverages.
引文
[1]ALVES P A,BRUM D M,ANDRADE E C,et al.Determination of synthetic dyes in selected foodstuffs by high performance liquid chromatography with UV-DAD detection[J].Food Chemistry,2008,107:489-496.
[2]SHEN Y X,ZHANG X M.Simultaneous determination of red and yellow artificial food colorants and carotenoid pigments in food products[J].Food Chemistry,2014,157(8):553-558.
[3]蔚向军,王俊娥,冯力.蛋白粉中6种合成色素同时测定的固相萃取-高效液相色谱法[J].职业与健康,2017,33(9):1182-1185.WEI Xiangjun,WANG Jun′e,FENG Li.Simultaneous determination of six synthetic pigments in protein powder by solid phase extraction-high performance liquid chromatography[J].Occupand and Health,2017,33(9):1182-1185.
[4]尤研.高效液相色谱法测定雪糕中6种合成色素[J].食品研究与开发,2017,38(12):147-152.YOU Yan.Determination of six synthetic pigments in ice cream by high performance liquid chromatography[J].Food Research and Development,2017,38(12):147-152.
[5]MARTIN F,OBERSON J M,MESCHIARI M,et al.Determination of 18water-soluble artificial dyes by LC-MS in selected matrices[J].Food Chemistry,2016,197:1249-1255.
[6]刘谦,李熙,吴新欢,等.高效液相色谱法同时测定复杂食品基质中8种合成色素的含量[J].理化检验-化学分册,2017,53(6):693-696.LIU Qian,LI Xi,WU Xinhuan,et al.Simultaneous determination of 8kinds of synthetic colorants in complex food matrix by HPLC[J].Chemical Analysis,2017,53(16):693-696.
[7]KARNIKOLOPOULOS G,GERAKIS A,PAPADOPOULOU K,et al.Determination of synthetic food colorants in fish product by an HPLC-DAD method[J].Food Chemistry,2015,77:97-203.
[8]YUAN Y,ZHAO X,QIAO M,et al.Determination of sunset yellow in soft drinks based on fluorescence quenching of carbon dots[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,167:106-110.
[9]AADRADE F I,GUEDES M I,VIEIRA I G,et al.Determination of synthetic food dyes in commercial soft drinks by TLC and ionpair HPLC[J].Food Chemistry,2014,157(1):193-198.
[10]王丽芳,丁晓静,谢娜,等.毛细管电泳法测定糖果及调制酒中10种人工合成色素[J].食品科学,2014,35(14):145-150.WANG Lifang,DING Xiaojing,XIE Na,et al.Simultaneous determination of ten synthetic food colorants in alcoholic beverages and candies by capillary electrophoresis[J].Food Science,2014,35(14):145-150.
[11]BONAN S,FEDRIZZI G,MENOTTA S,et al.Simultaneous determination of synthetic dyes in foodstuffs and beverages by high-performance liquid chromatography coupled with diode-array detector[J].Dyes and Pigments,2013,99(1):36-40.
[12]李沙,何洪亮.果汁饮料中7种常见人工合成色素的高效液相色谱快速测定法[J].职业与健康,2017,33(15):2078-2080.LI Sha,HE Hongliang.Rapid determination of 7synthetic pigments in juice by high performance liquid chromatography[J].Occupand and Health,2017,33(15):2078-2080.
[13]STACHOVA I,LHOTSKA I,SOLICH P,et al.Determination of green,blue and yellow artificial food colorants and their abuse in herb-coloured green easter beers on tap[J].Food Additives&Contaminants,2016,33(7):1139-1146.
[14]余雪婵,孙胜枚,梁丽萍.高效液相色谱法测定酱料、酱油中的诱惑红含量的研究[J].中国调味品,2016,41(5):51-56.YU Xuechan,SUN Shengmei,LIANG Liping.Research on detection of allura red content in sauce and soy sauce by HPLC[J].China Condiment,2016,41(5):51-56.
[15]蒋树新,刘华良,马永建,等.在线固相萃取-高效液相色谱检测饮料中5种食用合成色素[J].分析测试学报,2016,35(12):1581-1585.JIANG Shuxin,LIU Hualiang,MA Yongjian,et al.Determination of 5edible synthetic pigments in drinks by online solid-phase extraction coupled with high performance liquid chromatgraphy[J].Journal of Instrumental Analysis,2016,35(12):1581-1585.
[2]SHEN Y X,ZHANG X M.Simultaneous determination of red and yellow artificial food colorants and carotenoid pigments in food products[J].Food Chemistry,2014,157(8):553-558.
[3]蔚向军,王俊娥,冯力.蛋白粉中6种合成色素同时测定的固相萃取-高效液相色谱法[J].职业与健康,2017,33(9):1182-1185.WEI Xiangjun,WANG Jun′e,FENG Li.Simultaneous determination of six synthetic pigments in protein powder by solid phase extraction-high performance liquid chromatography[J].Occupand and Health,2017,33(9):1182-1185.
[4]尤研.高效液相色谱法测定雪糕中6种合成色素[J].食品研究与开发,2017,38(12):147-152.YOU Yan.Determination of six synthetic pigments in ice cream by high performance liquid chromatography[J].Food Research and Development,2017,38(12):147-152.
[5]MARTIN F,OBERSON J M,MESCHIARI M,et al.Determination of 18water-soluble artificial dyes by LC-MS in selected matrices[J].Food Chemistry,2016,197:1249-1255.
[6]刘谦,李熙,吴新欢,等.高效液相色谱法同时测定复杂食品基质中8种合成色素的含量[J].理化检验-化学分册,2017,53(6):693-696.LIU Qian,LI Xi,WU Xinhuan,et al.Simultaneous determination of 8kinds of synthetic colorants in complex food matrix by HPLC[J].Chemical Analysis,2017,53(16):693-696.
[7]KARNIKOLOPOULOS G,GERAKIS A,PAPADOPOULOU K,et al.Determination of synthetic food colorants in fish product by an HPLC-DAD method[J].Food Chemistry,2015,77:97-203.
[8]YUAN Y,ZHAO X,QIAO M,et al.Determination of sunset yellow in soft drinks based on fluorescence quenching of carbon dots[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2016,167:106-110.
[9]AADRADE F I,GUEDES M I,VIEIRA I G,et al.Determination of synthetic food dyes in commercial soft drinks by TLC and ionpair HPLC[J].Food Chemistry,2014,157(1):193-198.
[10]王丽芳,丁晓静,谢娜,等.毛细管电泳法测定糖果及调制酒中10种人工合成色素[J].食品科学,2014,35(14):145-150.WANG Lifang,DING Xiaojing,XIE Na,et al.Simultaneous determination of ten synthetic food colorants in alcoholic beverages and candies by capillary electrophoresis[J].Food Science,2014,35(14):145-150.
[11]BONAN S,FEDRIZZI G,MENOTTA S,et al.Simultaneous determination of synthetic dyes in foodstuffs and beverages by high-performance liquid chromatography coupled with diode-array detector[J].Dyes and Pigments,2013,99(1):36-40.
[12]李沙,何洪亮.果汁饮料中7种常见人工合成色素的高效液相色谱快速测定法[J].职业与健康,2017,33(15):2078-2080.LI Sha,HE Hongliang.Rapid determination of 7synthetic pigments in juice by high performance liquid chromatography[J].Occupand and Health,2017,33(15):2078-2080.
[13]STACHOVA I,LHOTSKA I,SOLICH P,et al.Determination of green,blue and yellow artificial food colorants and their abuse in herb-coloured green easter beers on tap[J].Food Additives&Contaminants,2016,33(7):1139-1146.
[14]余雪婵,孙胜枚,梁丽萍.高效液相色谱法测定酱料、酱油中的诱惑红含量的研究[J].中国调味品,2016,41(5):51-56.YU Xuechan,SUN Shengmei,LIANG Liping.Research on detection of allura red content in sauce and soy sauce by HPLC[J].China Condiment,2016,41(5):51-56.
[15]蒋树新,刘华良,马永建,等.在线固相萃取-高效液相色谱检测饮料中5种食用合成色素[J].分析测试学报,2016,35(12):1581-1585.JIANG Shuxin,LIU Hualiang,MA Yongjian,et al.Determination of 5edible synthetic pigments in drinks by online solid-phase extraction coupled with high performance liquid chromatgraphy[J].Journal of Instrumental Analysis,2016,35(12):1581-1585.