超干处理对柳枝稷种子活力及幼苗生长特性的影响
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摘要
研究超干处理对柳枝稷(Panicum virgatum L.)种子活力及幼苗生长特性的影响,为柳枝稷种子耐干性及贮藏提供理论基础。采用硅胶干燥法将柳枝稷种子含水量由9%分别降至7%,5%,3%,2%,1%,测定不同含水量条件下种子的活力以及种子萌发后第14 d幼苗的叶面积、根长、苗长、根冠比、鲜重、干重及相对电导率等指标的影响。柳枝稷种子含水量由对照9%降到2%的过程中,种子仍保持较高活力。含水量降至1%种子活力显著下降,但种子的相对电导率与种子活力没有显著相关性。超干处理后幼苗生长正常。柳枝稷种子属于正常型种子,其贮藏的安全水分下限为2%。超干处理并没有破坏柳枝稷种子细胞膜的完整性,也未对幼苗的生长造成损伤。
In order to provide a theoretical basis for desiccation tolerance and the storage of switchgrass(Panicum virgatum L.) seeds,we investigated the effect of ultra-dry treatment on the vigor of switchgrass seeds and seedling growth. The moisture content of switchgrass seeds was reduced to 7 %,5 %,4 %,3 %,2 % and 1 % from 9 %(control),respectively,with silica gel,and the vigor were detected when the seeds were re-moistened. And the leaf area,root length,seedling length,root/shoot ratio,fresh weight,dry weight and relative electrical conductivity were detected after the seed had germinated in 14 days. In the process of reducing the moisture content of switchgrass seeds from 9 % to 2 %,the seed vigor was kept relative high. When the moisture was reduced to 1 %,seed vigor decreased significantly. However,there was no significant difference between seed vigor and relative electrical conductivity. The seedling growth of the ultra-dry seeds was normal. Switchgrass seed was an orthodox seed and its low-limit of safe moisture content was 2 %. Ultra-dry treatment didn't damage the integrity of the cell membrane and the seedling growth.
In order to provide a theoretical basis for desiccation tolerance and the storage of switchgrass(Panicum virgatum L.) seeds,we investigated the effect of ultra-dry treatment on the vigor of switchgrass seeds and seedling growth. The moisture content of switchgrass seeds was reduced to 7 %,5 %,4 %,3 %,2 % and 1 % from 9 %(control),respectively,with silica gel,and the vigor were detected when the seeds were re-moistened. And the leaf area,root length,seedling length,root/shoot ratio,fresh weight,dry weight and relative electrical conductivity were detected after the seed had germinated in 14 days. In the process of reducing the moisture content of switchgrass seeds from 9 % to 2 %,the seed vigor was kept relative high. When the moisture was reduced to 1 %,seed vigor decreased significantly. However,there was no significant difference between seed vigor and relative electrical conductivity. The seedling growth of the ultra-dry seeds was normal. Switchgrass seed was an orthodox seed and its low-limit of safe moisture content was 2 %. Ultra-dry treatment didn't damage the integrity of the cell membrane and the seedling growth.
引文
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[24]孙爱清,高荣岐,尹燕枰.种子超干贮藏研究进展[J].山东农业大学学报(自然科学版),2000,31(3):325-329
[25]何学青,陶奇波,沙亚.海拉提,等.柳枝稷种子活力及幼苗生长对人工老化处理的响应[J].草地学报,2016,24(4):834-840
[26]霍平慧,李剑峰,师尚礼,等.盐胁迫对超干处理苜蓿种子萌发及幼苗生长的影响[J].草原与草坪,2011,31(1):13-18
[27]陈鹏,肖亮,关志华.菜豆种子的超干研究[J].西北植物学报,2005,25(7):1463-1466
[28]王爱君,王志敏,汤青林,等.超干处理对种子生物膜及种子活力的影响[J].南方农业,2011,5(4):52-54
[2]Andrea M.Switchgrass:a valuable biomass crop for energy[M].London:Springer-Verlag,2012:1
[3]Parrish D J,Fike J H.The biology and agronomy of switchgrass for biofuels[J].Critical Reviews in Plant Sciences,2005,24(5-6):423-459
[4]胡松梅,龚泽修,蒋道松.生物能源植物柳枝稷简介[J].草业科学,2008,25(6):29-33
[5]David T,Robert S,Jonathan A F,et al.Beneficial biofuels-the food,energy,and environment trilemma[J].Science,2009,325:270-271
[6]Eric K A,Allen S P,Thomas B V,et al.Nitrogen fertility and harvest management of switchgrass for sustainable bioenergy feedstock production in Illinois[J].Industrial Crops and Products,2013,48:19-27
[7]刘吉利,朱万斌,谢光辉,等.能源作物柳枝稷研究进展[J].草业学报,2009,18(3):232-240
[8]张红生,胡晋.种子学[M].北京:科学出版社,2012:195
[9]Hong T D,Ellis R H,Astley D,et al.Survival and vigour of ultra-dry seeds after ten years of hermetic storage[J].Seed Science and Technology,2005,33(2):449-460
[10]陈志宏,李晓芳,贠旭疆,等.种子超干贮存研究进展[J].草业科学,2011,28(2):296-303
[11]李鑫,王倩.种子超干贮藏研究进展[J].中国农学通报,2005,21(7):73-76
[12]刘大林,刘芳,高洪文,等.超干贮藏新麦草种子若干生理生化特性的研究[J].扬州大学学报(农业与生命科学版),2011,32(1):35-40
[13]姜义宝,郑秋红,王成章,等.超干贮藏对菊苣种子活力与抗氧化性的影响[J].草业学报,2009,18(5):93-97
[14]邹冬梅.超干贮藏对柱花草种子的影响[J].热带作物学报,2005,26(3):30-33
[15]徐炜,李晓芳,陈志宏,等.常温下超干贮藏对披碱草种子生理生化特性的影响[J].草地学报,2010,18(3):399-404
[16]李玉荣,苏红田,毛培胜,等.超干处理对山竹岩黄芪种子活力的影响[J].草地学报,2017,25(1):130-134
[17]李玉红,陈鹏,王绮,等.菠菜种子超干贮藏后抗老化作用的生理特性[J].西北农林科技大学学报(自然科学版),2005,33(8):96-98
[18]陈勇令,陈海荣.种子的超干贮藏研究概况[J].上海农业科技,2006,2:20-21
[19]李玉荣,苏红田,毛培胜,等.植物种子超干贮藏研究进展[J].种子,2011,30(12):53-57
[20]程红炎.种子超干贮藏技术的研究背景和现状[J].云南植物研究,2005,27(2):113-124
[21]王彦荣,孙建华,余玲,等.GB/T 2930.4-2001牧草种子检验规程[S].北京:中国标准出版社,2001
[22]赵鹏,张轶婷,郝丽珍,等.超干处理对沙芥属蔬菜种子活力及抗氧化代谢的影响[J].中国农业科学,2013,46(2):334-342
[23]曾广文,朱诚,胡家恕,等.红花种子超干期间自由基和水分状态的研究[J].浙江农业大学学报,1998,24(2):111-115
[24]孙爱清,高荣岐,尹燕枰.种子超干贮藏研究进展[J].山东农业大学学报(自然科学版),2000,31(3):325-329
[25]何学青,陶奇波,沙亚.海拉提,等.柳枝稷种子活力及幼苗生长对人工老化处理的响应[J].草地学报,2016,24(4):834-840
[26]霍平慧,李剑峰,师尚礼,等.盐胁迫对超干处理苜蓿种子萌发及幼苗生长的影响[J].草原与草坪,2011,31(1):13-18
[27]陈鹏,肖亮,关志华.菜豆种子的超干研究[J].西北植物学报,2005,25(7):1463-1466
[28]王爱君,王志敏,汤青林,等.超干处理对种子生物膜及种子活力的影响[J].南方农业,2011,5(4):52-54