碳点在中药活性组分标记领域的应用
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摘要
目的探讨应用碳点标记芥子碱硫氰酸盐,以明确其分子作用机制的可行性。方法以碳点为分子标记物,对芥子碱硫氰酸盐标记,进行细胞培养实验。以激光共聚焦成像技术观察碳点标记后的芥子碱硫氰酸盐的细胞的成像效果,进而以CCK-8法检验碳点及标记后的芥子碱硫氰酸盐的细胞毒性。结果采用共聚焦皿培养Ha Ca T细胞株。以空白碳点作为对照样,碳点标记的芥子碱硫氰酸盐作为实验样,激光共聚焦显微镜下观察,显绿光荧光。随着培养时间增加,荧光信号均逐渐增强,提示Ha Ca T细胞对芥子碱硫氰酸盐的摄入量越来越大。结论碳量子点可以有效标记芥子碱硫氰酸盐,有助于揭示芥子碱硫氰酸盐的分子作用机制。
Objective To investigate the feasibility of using carbon dots as a marker of sinapine thiocyanate,and to clarify its molecular mechanism.Methods During the cell culture experiment,the intervention of the carbon dots and carbon dots labeled sinapine thiocyanate were performed respectively.The imaging effect of the sinapine thiocyanate labeled with carbon dots was observed by confocal laser scanning technique.The cytotoxicity of carbon dots and labeled sinapine thiocyanate were examined by CCK-8 method.Results Ha Ca T cell line was cultured with confocal dishes.Using blank carbon dots as the control,the thiocyanate labeled with carbon dots was used as the experimental sample,and the green fluorescence was observed by confocal laser scanning microscope.With the increase of culture time,the fluorescence signal increased gradually.This indicated that the intake of mustard and thiocyanate was more and more important in Ha Ca T cells.Conclusion The carbon dots can be used as an effective marker for sinapine thiocyanate,which will help to reveal the molecular mechanism of sinapine thiocyanate.
Objective To investigate the feasibility of using carbon dots as a marker of sinapine thiocyanate,and to clarify its molecular mechanism.Methods During the cell culture experiment,the intervention of the carbon dots and carbon dots labeled sinapine thiocyanate were performed respectively.The imaging effect of the sinapine thiocyanate labeled with carbon dots was observed by confocal laser scanning technique.The cytotoxicity of carbon dots and labeled sinapine thiocyanate were examined by CCK-8 method.Results Ha Ca T cell line was cultured with confocal dishes.Using blank carbon dots as the control,the thiocyanate labeled with carbon dots was used as the experimental sample,and the green fluorescence was observed by confocal laser scanning microscope.With the increase of culture time,the fluorescence signal increased gradually.This indicated that the intake of mustard and thiocyanate was more and more important in Ha Ca T cells.Conclusion The carbon dots can be used as an effective marker for sinapine thiocyanate,which will help to reveal the molecular mechanism of sinapine thiocyanate.
引文
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[20]唐秋玲,赵晓欢,杨明锡,等.色氨酸碳点的合成及在细胞生物学成像中的应用[J].口腔医学研究,2017,33(11):1213-1217.
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[22]王胜达,袁楠,朱志峰,等.荧光碳点的合成、性质和应用[J].影像科学与光化学,2016,34(3):203-218.
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[24]邓祥,黄小梅,祁文静,等.玛卡荧光碳点的合成及用于苦味酸的荧光检测[J].分析化学,2016,44(12):1880-1886.
[25]李志英,闫慧云,刘璐璐,等.柠檬酸修饰荧光碳点的合成及对中草药总黄酮的检测[J].化学研究与应用,2017,29(9):1352-1356.
[2]王琳琳,庞立健,白晓红,等.冬病夏治贴敷疗法防治儿童反复肺炎临床疗效观察[J].中国针灸,2016,36(3):261-265.
[3]徐涵斌,何勇,潘浩,等.宋南昌应用三伏灸贴发泡疗法防治支气管哮喘经验[J].中国针灸,2016,36(6):633-636.
[4]Michalet X,Pinaud FF,Bentolila LA,et al.Quantum dots for live cells,in vivo imaging,and diagnostics[J].Science,2005,307(5709):538-544.
[5]De M,Ghosh PS,Rotello VM.Applications of nanoparticles in biology[J].Ad V Mater,2008,20(22):4225-4241.
[6]Bruchez M Jr,Moronne M,Gin P,et al.Semiconductor nanocrystals as fluorescent biological labels[J].Science,1998,281(5385):2013-2016.
[7]李鸿程,骆文茹,陶涌,等.Cd Se量子点对雄性泥鳅的毒性效应研究[J].中国科学,2009,39(10):1277-1284.
[8]陈幕飞,黄承志,蒲德永,等.Cd Se/Zn S量子点对斑马鱼胚胎发育的毒性效应[J].环境科学,2015,36(2):719-726.
[9]Liu N,Mu Y,Chen Y,et al.Degradation of aqueous synthesized Cd Te/Zn S quantum dots in mice:differential blood kinetics and biodistribution of cadmium and tellurium[J].Part Fibre Toxicol,2013,10:37.
[10]Zhao F,Meng H,Yan L,et al.Nanosurface chemistry and dose govern the bioaccumulation and toxicity of carbon nanotubes,metal nanomaterials and quantum dots in vivo[J].Science Bulletin,2015,60(1):3-20.
[11]Du W,Xu X,Hao H,et al.Green synthesis of fluorescent carbon quantum dots and carbon spheres from pericarp[J].SCI Chin Chem,2015,58(5):863-870.
[12]Loh KP,Bao Q,Eda G,et al.Graphene oxide as a chemically tunable platform for optical applications[J].Nat Chem,2010,2(12):1015-1024.
[13]Guo Y,Wang Z,Shao H,et al.Hydrothermal synthesis of highly fluorescent carbon nanoparticles from sodium citrate and their use for the detection of mercury ions[J].Carbon,2013,52(2):583-589.
[14]Liu H,Wang Q,Shen G,et al.A multifunctional ribonuclease A-conjugated carbon dot cluster nanosystem for synchronous cancer imaging and therapy[J].Nanoscale Res Lett,2014,9(1):397.
[15]Woo DJ,Sneed B,Peerally F,et al.Synthesis of nanodiamondreinforced aluminum metal composite powders and coatings using high-energy ball milling and cold spray[J].Carbon,2013,63(15):404-415.
[16]Chen A,Yu Y,Li Y,et al.Synthesis of macro-mesoporous carbon materials and hollow core/mesoporous shell carbon spheres as supercapacitors[J].J Mater Sci,2016,51(9):4601-4608.
[17]木合塔尔·吐尔洪,徐阳,尹学博.碳点的研究进展[J].分析化学,2017,45(1):139-150.
[18]Bourlinos AB,Zbo il R,Petr J,et al.Luminescent Surface Quaternized Carbon Dots[J].Chem Mater,2012,24(1):6-8.
[19]刘飞,薛冰,顾月清.荧光标记在药物分析领域的应用进展[J].药学进展,2011,35(2):64-69.
[20]唐秋玲,赵晓欢,杨明锡,等.色氨酸碳点的合成及在细胞生物学成像中的应用[J].口腔医学研究,2017,33(11):1213-1217.
[21]车望远,刘长军,杨焜,等.荧光碳点的制备和性质及其应用研究进展[J].复合材料学报,2016,33(3):431-450.
[22]王胜达,袁楠,朱志峰,等.荧光碳点的合成、性质和应用[J].影像科学与光化学,2016,34(3):203-218.
[23]车望远,刘长军,杨焜,等.荧光碳点的制备和性质及其应用研究进展[J].复合材料学报,2016,33(3):431-450.
[24]邓祥,黄小梅,祁文静,等.玛卡荧光碳点的合成及用于苦味酸的荧光检测[J].分析化学,2016,44(12):1880-1886.
[25]李志英,闫慧云,刘璐璐,等.柠檬酸修饰荧光碳点的合成及对中草药总黄酮的检测[J].化学研究与应用,2017,29(9):1352-1356.