苯并噻重氮结合不同包装材料对金针菇贮藏品质及酶活性的影响
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摘要
以金针菇为原料,研究抗性材料苯并噻重氮结合不同包装材料处理对金针菇贮藏品质及相关酶活性的影响。结果表明,苯并噻重氮(acibenzolar-S-methyl,ASM)结合不同包装材料处理能有效降低金针菇呼吸强度、抑制丙二醛的产生、减轻失重率、保留VC,并维持固有色泽;能提高金针菇过氧化物酶(POD)和超氧化物歧化酶(SOD)活性,增强金针菇清除活性氧自由基的能力;同时能降低多酚氧化酶(PPO)活性以减少酶促褐变。最后,实验得到0. 75 mmol/L ASM与PE膜无孔包装结合对保持金针菇贮藏品质效果最优,为金针菇的贮藏保鲜提供了数据参考。
The effects of Acibenzolar-S-methyl( ASM) on storage quality and enzyme activity of Flammulina velutipes were investigated. The results indicated that ASM could effectively reduce the respiration intensity of Flammulina velutipes and inhibit the formation of malondialdehyde. Besides,ASM reduced the weight loss rate,retained vitamin C and nature color. The activities of peroxidase and superoxide diamutase also improved,which enhanced the ability of Flammulina velutipes to eliminate reactive oxygen free radicals. Moreover,the activity of polyphenol oxidase was lowered and hence reduced enzymatic browning. Lastly,0. 75 mmol/L ASM was found to have the best preservative effects when combined with PE film packing,which provides reference for storing Flammulina velutipes.
The effects of Acibenzolar-S-methyl( ASM) on storage quality and enzyme activity of Flammulina velutipes were investigated. The results indicated that ASM could effectively reduce the respiration intensity of Flammulina velutipes and inhibit the formation of malondialdehyde. Besides,ASM reduced the weight loss rate,retained vitamin C and nature color. The activities of peroxidase and superoxide diamutase also improved,which enhanced the ability of Flammulina velutipes to eliminate reactive oxygen free radicals. Moreover,the activity of polyphenol oxidase was lowered and hence reduced enzymatic browning. Lastly,0. 75 mmol/L ASM was found to have the best preservative effects when combined with PE film packing,which provides reference for storing Flammulina velutipes.
引文
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[21] TAKAHAMA U,ONIKI T. A peroxidase/phenolics/ascorbate system can scavenge hydrogen peroxide in plant cells[J]. Physiologia Plantarum,1997,101(4):845-852.
[22]李成忠,孙燕,杜庆平.黄连木根系抗氧化酶系对干旱胁迫的响应[J].林业科技开发,2008,22(2):57-60.
[23]王成涛,王昌涛,赵磊,等.不同氧分压条件下金针菇贮藏保鲜相关酶活性的研究[J].食品科学,2011,32(8):300-304.
[2] LEUNG M Y,FUNG K P,CHOY Y M. The isolation and characterization of an immunomodula-tory and anti-tumor polysaccharide preparation from Flammulina velutipes.[J]. Immunopharmacology,1997,35(3):255-263.
[3] KAMASUKA T,MOMOKI Y,SAKAI S. Antitumor activity of polysaccharide fractions prepared from some strains of Basidiomycetes[J]. Gan,1968,59(5):443-445.
[4]闵三弟,臧珍娣.金针菇抗癌有效成分研究进展[J].食用菌,1992(5):44.
[5] ZHOU Kaisong,PENG Junfeng,CHANG Ning,et al.Purification and crystallization of flammulin,a basic protein with anti-tumor activities from Flammulina velutipes[J].Chinese Chemical Letters,2003,14(7):713-716.
[6]陈晓东,张伟阳,张琪,等.生物可降解聚乳酸薄膜对金针菇保鲜效果的研究[J].食品工业科技,2013,34(22):306-309;319.
[7] BURTON K S. The quality and storage life of Agaricus bisporus,Mushroom Science X[C]//Proceeding of 12th International Congress on the Science and Cultivation of Edible Fungi,Germany:Braunschwey,1989:683-688.
[8] LI D Q,QIN X Y,TIAN P P,et al. Toughening and its association with the postharvest quality of king oyster mushroom(Pleurotus eryngii)stored at low temperature[J].Food Chemistry,2016(196):1092-1100
[9]何永梅,皮登高.食用菌的几种贮藏保鲜方法[J].保鲜与加工,2009(1):3-4.
[10]祝美云,王安建,侯传伟,等.食用菌双孢菇的化学保鲜试验[J].浙江农业科学,2008,1(6):702-704.
[11]孙娅,李志强,王毓宁,等.采后钙处理对双孢菇贮藏生理的影响[J].食品工业科技,2013,34(2):322-326
[12]王毓宁,胡花丽,孙娅,等.保鲜剂对双孢菇褐变的影响[J].江苏农业学报,2012,28(5):1141-1145
[13]付伟,焦云红,王更先,等.复方保鲜剂在平菇采后保鲜中的应用研究[J].北方园艺,2014(18):151-154
[14]吴秀华,关迎春,杨忠辉.食用菌化学保鲜法[J].农民致富之友,2006(2):30.
[15]刘红霞. 1-MCP,BTH和PHC对桃果(Prunus persica L.)采后衰老的调控作用及诱导抗病机理的研究[D].北京:中国农业大学,2004.
[16]刘亭,钱政江,屈红霞,等.利用LI-6262 CO2/H2O分析仪测定果蔬的呼吸强度[J].保鲜与加工,2010,10(4):55-56.
[17] ARES G,PARENTELLI C,GMBARO A,et al. Sensory shelf life of shiitake mushrooms stored under passive modified atmosphere[J]. Postharvest Biology&Technology,2006,41(2):191-197.
[18]曹建康,姜微波.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.
[19] CHEN C,XUE K S,YAN L I,et al. Purification and characterization of flammulin,a basic protein with antitumor activities from Flammulina velutipes[J]. Journal of Chinese Pharmaceu-tical Sciences,2003,12(2):60-65.
[20] TANG X,BAI Y,DUONG A,et al. Formaldehyde in China:Production,consumption,exposure levels,and health effects[J]. Environment International,2009,35(8):1 210-1 224.
[21] TAKAHAMA U,ONIKI T. A peroxidase/phenolics/ascorbate system can scavenge hydrogen peroxide in plant cells[J]. Physiologia Plantarum,1997,101(4):845-852.
[22]李成忠,孙燕,杜庆平.黄连木根系抗氧化酶系对干旱胁迫的响应[J].林业科技开发,2008,22(2):57-60.
[23]王成涛,王昌涛,赵磊,等.不同氧分压条件下金针菇贮藏保鲜相关酶活性的研究[J].食品科学,2011,32(8):300-304.