2017年瑞安市副溶血性弧菌监测结果分析
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摘要
目的分析2017年瑞安市副溶血性弧菌监测结果,揭示其血清型和毒力基因携带情况。方法按照国家标准GB 4789.7—2013进行分离检测、血清分型,用荧光PCR方法检测其tdh和trh毒力基因。结果在130份水产品中检出56株副溶血性弧菌,总阳性率为43.1%。54株分离自腹泻患者的副溶血性弧菌中,检出32株O4:KUT(K为不能分型)血清型(59.3%),14株O3:K6(25.9%),2株O4:K8,5株O1:KUT,1株O1:K20。所有分离自水产品的菌株tdh、trh均为阴性。分离自腹泻患者的菌株tdh毒力基因携带率为93.3%(28/30)。结论 O4:KUT已经超越O3:K6成为2017年瑞安地区副溶血性弧菌临床分离株的优势血清型。
Objective To analyze the monitoring result of Vibrio parahaemolyticus in Rui′an and investigate the serotype and virulence genes in 2017. Methods Vibrio parahaemolyticus were isolated and serotyped according to national standard GB 4789.7—2013. Virulence genes tdh and trh were detected by fluorescence PCR. Results Totally 56 isolates of Vibrio parahaemolyticus were detected among 130 seafood samples, with the positive ratio of 43.1%. Among the 54 clinical isolates, 32 strains of O4:KUT(59.3%), 14 strains of O3:K6(25.9%), 2 strains of O4:K8, 5 strains of O1:KUT, 1 strain of O1:K20 were detected. None of these isolates carried virulence gene tdh or trh. 93.3%(28/30) of clinical isolates carried virulence gene tdh. Conclusion In 2017, serotype O4:KUT was instead of O3:K6 to be the predominate serotype of clinical Vibrio parahaemolyticus isolates in Rui′an.
Objective To analyze the monitoring result of Vibrio parahaemolyticus in Rui′an and investigate the serotype and virulence genes in 2017. Methods Vibrio parahaemolyticus were isolated and serotyped according to national standard GB 4789.7—2013. Virulence genes tdh and trh were detected by fluorescence PCR. Results Totally 56 isolates of Vibrio parahaemolyticus were detected among 130 seafood samples, with the positive ratio of 43.1%. Among the 54 clinical isolates, 32 strains of O4:KUT(59.3%), 14 strains of O3:K6(25.9%), 2 strains of O4:K8, 5 strains of O1:KUT, 1 strain of O1:K20 were detected. None of these isolates carried virulence gene tdh or trh. 93.3%(28/30) of clinical isolates carried virulence gene tdh. Conclusion In 2017, serotype O4:KUT was instead of O3:K6 to be the predominate serotype of clinical Vibrio parahaemolyticus isolates in Rui′an.
引文
[1] 梅玲玲, 龚璞, 占利, 等. 浙江省副溶血性弧菌O3:K6血清型菌种多位点序列分型研究[J]. 中国人兽共患病学报, 2014, 30(3): 278-281.
[2] 郑文龙, 王卓, 马洁, 等. 副溶血性弧菌病原学和分子流行病学流行特征研究进展[J]. 疾病监测, 2015, 30(4): 337-339.
[3] 中华人民共和国国家卫生和计划生育委员会. GB 4789.7—2013 食品安全国家标准食品微生物学检验副溶血性弧菌检验[S]. 北京: 中国标准出版社, 2014.
[4] 高玮, 潘迎洁, 赵勇, 等. 上海市水产品中副溶血性弧菌的分离、鉴定毒力基因和血清型分布[J]. 食品与生物科技学报, 2011, 30(2): 278-282.
[5] 袁瑞, 付云, 张鹏. 2014-2016年湖州市水产品与临床腹泻病例中副溶血性弧菌的分布特征[J]. 中国食品卫生杂志, 2017, 29(3): 294-298.
[6] 姜照, 张桂芳, 伍海燕, 等. 2012年-2016年烟台市627份海产品副溶血性弧菌监测结果分析[J]. 现代预防医学, 2017, 44(17): 3125-3127.
[7] DePaola A, Nordstrom JL, Bowers JC, et al. Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters[J]. Appl Environ Microb, 2003, 69(3): 1521-1526.
[8] Alam M, Chowdhury WB, Bhuiyan NA, et al. Serogroup,virulence and genetic traits of Vibrio parahaemolyticus in the Estuarine ecosystem of Bangladesh[J]. Appl Environ Microb, 2009, 75(19): 6268-6274.
[9] 杨丽华, 陈敏, 王小光, 等. 上海市闵行地区副溶血性弧菌分子流行病学特征分析[J]. 中国食品卫生杂志, 2012, 24(6): 523-528.
[10] 谢红毅, 叶鸿雁, 周方满, 等. 副溶血性弧菌海产品及临床分离株毒力基因及多位点序列分型分析[J]. 中国卫生检验杂志, 2018, 28(2): 169-171.
[11] 李娜, 刘敏芝, 叶环环, 等. 瑞安市副溶血性弧菌临床分离株的血清分型和毒力基因研究[J]. 延边医学, 2015, 10(171): 266-267.
[12] Vongxay K, Pan Z, Zhang X, et al. Occurrence of pandemic clones of Vibrio parahaemolyticus isolates from seafood and clinical samples in a Chinese coastal province[J]. Foodborne Pathog Dis, 2008, 5(2): 127-134.
[13] Vuddhakul V, Chowdhury A, Laohapretthisan V, et al. Isolation of a pandemic O3:K6 clone of a Vibrio parahaemolyticus strain from environmental and clinical sources in Thailand[J]. Appl Environ Microb, 2000, 66(6): 2685-2689.
[14] 刘李, 许欣, 段永翔, 等. 深圳市副溶血性弧菌血清型及其毒力基因特征研究[J]. 中国热带医学, 2009, 9(8): 1399-1340.
[15] 商晓春, 詹丽, 帅慧群, 等. 杭州市下城区副溶血性弧菌菌群菌型分布、毒力基因及耐药性研究[J]. 中国卫生检验杂志, 2011, 21(11): 2779-2082.
[2] 郑文龙, 王卓, 马洁, 等. 副溶血性弧菌病原学和分子流行病学流行特征研究进展[J]. 疾病监测, 2015, 30(4): 337-339.
[3] 中华人民共和国国家卫生和计划生育委员会. GB 4789.7—2013 食品安全国家标准食品微生物学检验副溶血性弧菌检验[S]. 北京: 中国标准出版社, 2014.
[4] 高玮, 潘迎洁, 赵勇, 等. 上海市水产品中副溶血性弧菌的分离、鉴定毒力基因和血清型分布[J]. 食品与生物科技学报, 2011, 30(2): 278-282.
[5] 袁瑞, 付云, 张鹏. 2014-2016年湖州市水产品与临床腹泻病例中副溶血性弧菌的分布特征[J]. 中国食品卫生杂志, 2017, 29(3): 294-298.
[6] 姜照, 张桂芳, 伍海燕, 等. 2012年-2016年烟台市627份海产品副溶血性弧菌监测结果分析[J]. 现代预防医学, 2017, 44(17): 3125-3127.
[7] DePaola A, Nordstrom JL, Bowers JC, et al. Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters[J]. Appl Environ Microb, 2003, 69(3): 1521-1526.
[8] Alam M, Chowdhury WB, Bhuiyan NA, et al. Serogroup,virulence and genetic traits of Vibrio parahaemolyticus in the Estuarine ecosystem of Bangladesh[J]. Appl Environ Microb, 2009, 75(19): 6268-6274.
[9] 杨丽华, 陈敏, 王小光, 等. 上海市闵行地区副溶血性弧菌分子流行病学特征分析[J]. 中国食品卫生杂志, 2012, 24(6): 523-528.
[10] 谢红毅, 叶鸿雁, 周方满, 等. 副溶血性弧菌海产品及临床分离株毒力基因及多位点序列分型分析[J]. 中国卫生检验杂志, 2018, 28(2): 169-171.
[11] 李娜, 刘敏芝, 叶环环, 等. 瑞安市副溶血性弧菌临床分离株的血清分型和毒力基因研究[J]. 延边医学, 2015, 10(171): 266-267.
[12] Vongxay K, Pan Z, Zhang X, et al. Occurrence of pandemic clones of Vibrio parahaemolyticus isolates from seafood and clinical samples in a Chinese coastal province[J]. Foodborne Pathog Dis, 2008, 5(2): 127-134.
[13] Vuddhakul V, Chowdhury A, Laohapretthisan V, et al. Isolation of a pandemic O3:K6 clone of a Vibrio parahaemolyticus strain from environmental and clinical sources in Thailand[J]. Appl Environ Microb, 2000, 66(6): 2685-2689.
[14] 刘李, 许欣, 段永翔, 等. 深圳市副溶血性弧菌血清型及其毒力基因特征研究[J]. 中国热带医学, 2009, 9(8): 1399-1340.
[15] 商晓春, 詹丽, 帅慧群, 等. 杭州市下城区副溶血性弧菌菌群菌型分布、毒力基因及耐药性研究[J]. 中国卫生检验杂志, 2011, 21(11): 2779-2082.