枯草芽孢杆菌发酵玉米肽供体的筛选
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摘要
为研究不同供体修饰玉米肽(Corn Peptide,CP)所得产物的抗氧化活性,以CP为底物,通过添加酸类(琥珀酸酐、乙二胺四乙酸、乙酸酐、乙酸、柠檬酸、草酸)、糖类(葡萄糖、乳糖、半乳糖、甘露糖、果糖)和金属离子(硫酸铁、硫酸铜、硫酸镁、硫酸锌、硫酸锰、硫酸钙)等供体,以枯草芽孢杆菌(Bacillus subtilis) ls-45为菌种,DPPH转化率为指标进行修饰供体的筛选,研究发酵后不同剂量(高、中、低) CP修饰产物体内、体外抗氧化活性。结果表明:利用枯草芽孢杆菌发酵后,三类供体对CP修饰效果整体表现为:糖类>酸类>金属离子,经筛选修饰CP的最佳供体为葡萄糖,当投料比为8∶1时,DPPH转化率达到79.84%。将发酵后得到的最优修饰产物(玉米肽-葡萄糖(CP-G))进行小鼠体内抗氧化活性实验发现,CP中、高剂量组和CP-G低、中、高剂量组均显著(p <0.05)提高血清和肝脏中的SOD活性。CP和CP-G低、中、高剂量组均显著(p <0.05)提高血清中的GSH-Px活性; CP低、中、高剂量组,CP-G中、高剂量组均显著(p <0.05)提高肝脏中的GSH-Px活性,说明经葡萄糖修饰后的CP具有较高的抗氧化活性。
In order to study the antioxidant activity of corn peptide modified with different donors,the acid( succinic anhydride,acetic anhydride,acetic acid,citric acid,oxalic acid),sugars( glucose,lactose,galactose,mannose,fructose) and metal ions( ferric sulfate,copper sulfate,magnesium sulfate,zinc sulfate,manganese sulfate,calcium sulfate) were added to corn peptide as substrate.Donors were screened with Bacillus subtilis ls-45 as strain,and DPPH conversion rate was as index.The antioxidant activity of products modified by corn peptide with different doses( high,medium and low) after fermentation in vivo and vitro was studied.The results showed that,the effect of corn peptide modification femented by B.subtilis was as follows: Carbohydrate> acid > metal ion. The best donor of corn peptide chelate was glucose. When the feed ratio was 8 ∶ 1,the conversion rate of DPPH reached 79.84%. The optimal modification product obtained after fermentation( corn peptide-glucose( CP-G)) was tested in mice for antioxidant activity. It was found that CP medium,high dose group and CP-G low,medium and high dose groups were significant( p < 0.05) increased SOD activity in serum and liver.The low,medium and high doses of CP and CP-G significantly( p < 0.05) increased serum GSH-Px activity,CP low,medium and high dose groups,CP-G medium and high dose groups were significant( p < 0.05) Increased GSH-Px activity in the liver,which indicated that the corn peptide-glucose modified product after fermentation could effectively improve the antioxidant activity in mice.
In order to study the antioxidant activity of corn peptide modified with different donors,the acid( succinic anhydride,acetic anhydride,acetic acid,citric acid,oxalic acid),sugars( glucose,lactose,galactose,mannose,fructose) and metal ions( ferric sulfate,copper sulfate,magnesium sulfate,zinc sulfate,manganese sulfate,calcium sulfate) were added to corn peptide as substrate.Donors were screened with Bacillus subtilis ls-45 as strain,and DPPH conversion rate was as index.The antioxidant activity of products modified by corn peptide with different doses( high,medium and low) after fermentation in vivo and vitro was studied.The results showed that,the effect of corn peptide modification femented by B.subtilis was as follows: Carbohydrate> acid > metal ion. The best donor of corn peptide chelate was glucose. When the feed ratio was 8 ∶ 1,the conversion rate of DPPH reached 79.84%. The optimal modification product obtained after fermentation( corn peptide-glucose( CP-G)) was tested in mice for antioxidant activity. It was found that CP medium,high dose group and CP-G low,medium and high dose groups were significant( p < 0.05) increased SOD activity in serum and liver.The low,medium and high doses of CP and CP-G significantly( p < 0.05) increased serum GSH-Px activity,CP low,medium and high dose groups,CP-G medium and high dose groups were significant( p < 0.05) Increased GSH-Px activity in the liver,which indicated that the corn peptide-glucose modified product after fermentation could effectively improve the antioxidant activity in mice.
引文
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[3]Yang Y,Tao G,Liu P,et al. Peptide with angiotensin I-converting enzyme inhibitory activity from hydrolyzed corn gluten meal[J]. Journal of Agricultural and Food Chemistry,2007,55(19):7891-7895.
[4]Huang W H,Sun J,He H,et al.Antihypertensive effect of corn peptides,produced by a continuous production in enzymatic membrane reactor,in spontaneously hypertensive rats[J]. Food Chemistry,2011,128(4):968-973.
[5]Guo H,Sun J,He H,et al. Antihepatotoxic effect of corn peptides against Bacillus Calmette-Guerin/lipopolysaccharideduced liver injury in mice[J]. Food and Chemical Toxicology,2009,47(10):2431-2435.
[6] Zhou K,Sun S,Canning C. Production and functional characterisation of antioxidative hydrolysates from corn protein via enzymatic hydrolysis and ultrafiltration[J].Food Chemistry,2012,135(3):1192-1197.
[7]周玲玲.玉米花生复合蛋白酶解产物生物活性及加工性能的研究[D].武汉:华中农业大学,2011.
[8]Liu H M,Shi G B,Ma Y F,et al. Optimization of the production of peanut antioxidative peptides and neutrase using liquid state fermentation[J]. Science&Technology of Food Industry,2014,35(18):175-179.
[9]陈星星,胡晓,李来好.抗疲劳肽的研究进展[J].食品工业科技,2015,36(4):365-369.
[10]Li J T,Zhang J L,He H,et al.Apoptosis in human hepatoma Hep G2 cells induced by corn peptides and its anti-tumor efficacy in H22 tumor bearing mice[J]. Food and Chemical Toxicology,2013,51:297-305.
[11]张智,刘慧,刘奇,等.C-锌螯合物结构表征及抗氧化活性分析[J].食品科学,2017,38(3):131-135.
[12]迟玉杰,朱秀清,李文滨,等.大豆蛋白质加工新技术[M].北京:科学出版社,2009:192-193.
[13]牟金秀.微生物发酵法生产C的研究[D].济南:齐鲁工业大学,2015.
[14]Zhuang H,Tang N,Dong S,et al.Optimisation of antioxidant peptide preparation from corn gluten meal[J]. Journal of the Science of Food and Agriculture,2013,93(13):3264-3270.
[15]孟繁磊,陈瑞战,张敏,等.刺五加多糖的提取工艺及抗氧化活性研究[J].食品科学,2010,31(10):168-174.
[16]Rodroguezcapote K,Tovell K,Holmes D,et al. Analytical evaluation of the diazyme glycated serum protein assay on the siemens Advia 1800:Comparison of results against Hb A1c for diagnosis and management of diabetes[J]. Journal of Diabetes Science&Technology,2015,9(2):192-199.
[17]姜苏薇,潘利华,徐学玲,等.水溶性大豆膳食纤维对高脂血症小鼠肾脏的抗氧化应激作用[J].食品科学,2011,32(19):240-243.