桑枝中三种氨基酸成分的薄层鉴别
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摘要
目的建立桑枝中氨基酸成分的薄层鉴别方法。方法以蒸馏水为提取溶剂对桑枝中氨基酸进行超声提取,并对展开系统[正丁醇-冰醋酸-水(4∶2.2∶3)、正丁醇-冰醋酸-水(4∶1∶5)、正丁醇-冰醋酸-水(4∶3∶1),正丁醇-冰醋酸-水(4∶2.2∶1),正丁醇-冰醋酸-水(6∶2.2∶1)]、点样量(1、2、3、4、5和6μL)、薄层板(硅胶G板、高效硅胶G板、硅胶GF254板、硅胶H板、聚酰胺薄膜)等薄层色谱条件进行考察。结果以正丁醇-冰乙酸-水(6∶2.2∶1)为展开系统,0.2%茚三酮为显色剂,硅胶G板为薄层板,混合氨基酸对照品溶液和供试品溶液的点样量分别是2μL和3μL,薄层层析法鉴别出桑枝中含有精氨酸,苏氨酸和缬氨酸,其薄层色谱图斑点清晰,专属性强。结论该方法简便可行,结果准确,可作为桑枝中氨基酸的薄层鉴别方法。
Objective To establish the methods for identification of amino acids in Ramulus Mori by TLC.Methods The amino acids was extracted by ultrasonic method with distilled water as solvent,the TLC conditions,such as the development system [n-butanol-acetic acid-water(4∶2.2∶3),n-butanol-acetic acid-water(4∶1∶5),n-butanol-acetic acid-water(4∶3∶1),n-butanol-acetic acid-water(4∶2.2∶1),nbutanol-acetic acid-water(6∶2.2∶1)],spotting sample volume(1,2,3,4,5 and 6μL),thin layer chromatography plate(G plate,efficient silica gel G plate,GF254 plate,H plate,polyamide film),were investigated.Results The TLC analysis indicated that the test samples contained arginine,threonine and valine.The spots were clear and its specificity was high when n-butanol-acetic acid-water(6∶2.2∶1)was used as the developing system,and 0.2% ninhydrin as chromogenic reagent,G plate was used as thin layer chromatography plate,and the spotting sample volume of amino acid mixed standard and sample were2μL and 3μL respectively.Conclusion The methods are simple,accurate and can be used as amino acids in Ramulus Mori identification.
Objective To establish the methods for identification of amino acids in Ramulus Mori by TLC.Methods The amino acids was extracted by ultrasonic method with distilled water as solvent,the TLC conditions,such as the development system [n-butanol-acetic acid-water(4∶2.2∶3),n-butanol-acetic acid-water(4∶1∶5),n-butanol-acetic acid-water(4∶3∶1),n-butanol-acetic acid-water(4∶2.2∶1),nbutanol-acetic acid-water(6∶2.2∶1)],spotting sample volume(1,2,3,4,5 and 6μL),thin layer chromatography plate(G plate,efficient silica gel G plate,GF254 plate,H plate,polyamide film),were investigated.Results The TLC analysis indicated that the test samples contained arginine,threonine and valine.The spots were clear and its specificity was high when n-butanol-acetic acid-water(6∶2.2∶1)was used as the developing system,and 0.2% ninhydrin as chromogenic reagent,G plate was used as thin layer chromatography plate,and the spotting sample volume of amino acid mixed standard and sample were2μL and 3μL respectively.Conclusion The methods are simple,accurate and can be used as amino acids in Ramulus Mori identification.
引文
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[4]孙莲,孟磊,阿布都许库尔·吐尔逊.气相色谱法测定新疆桑枝多糖中单糖组成及含量[J].安徽农业科学,2014,42(19):6207-6208.
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[10]甄攀,倪刚,郭伟强,等.川桑(Morus notabilis)枝条的药用化学成分分离鉴定[J].蚕业科学,2016,42(2):307-312.
[11]牛凤菊,周祉延.桑枝降血糖的活性部位研究[J].世界中西医结合杂志,2015,10(9):1219-1221.
[12]吴娱明,邹宇晓,廖森泰,等.桑枝提取物对实验高血脂症小鼠的降血脂作用初步研究[J].2005,31(3):348-350.
[13]章丹丹,凌摇霜,张洪平,等.桑枝总黄酮体外抗炎活性及机制研究[J].时珍国医国药,2010,21(11):2787-2890.
[14]邬灏,卢笑丛,王有为.桑枝多糖分离纯化及其免疫作用的初步研究[J].武汉植物学研究,2005,23(1):81-84.
[2]洪德志,时连根.桑枝多糖对糖尿病模型小鼠的降血糖作用[J].中国药理学与毒理学杂志,2012,26(6):806-809.
[3]郭福团,许雄伟,潘建峰,等.桑枝多糖对糖尿病肾病大鼠肾脏组织抗氧化作用的影响[J].中国医院药学杂志,2016,36(23):2058-2061.
[4]孙莲,孟磊,阿布都许库尔·吐尔逊.气相色谱法测定新疆桑枝多糖中单糖组成及含量[J].安徽农业科学,2014,42(19):6207-6208.
[5]刘超,史正琴,向伟,等.桑枝1-脱氧野尻霉素的提取及其含量与α-糖苷酶抑制活性的相关性分析[J].林业科学,2015,51(2):112-120.
[6]张波,李学刚,罗旭梅,等.桑枝提取物对α-葡萄糖苷酶活性及餐后血糖的影响[J].时珍国医国药,2014,25(6):1325-1327.
[7]孙莲,杜申道,李晓艳,等.桑枝中槲皮素和绿原酸的薄层色谱鉴别及抗氧化活性筛选[J].国际药学研究杂志,2016,43(3):548-551.
[8]姜乃珍,薄铭,吴志平,等.中药桑枝化学成分及药理活性研究进展[J].江苏蚕业,2006,28(2):4-7.
[9]李晓艳,杜申道,孙莲.桑枝中γ-氨基丁酸的鉴别与含量测定[J].国际药学研究杂志,2016,43(5):994-997.
[10]甄攀,倪刚,郭伟强,等.川桑(Morus notabilis)枝条的药用化学成分分离鉴定[J].蚕业科学,2016,42(2):307-312.
[11]牛凤菊,周祉延.桑枝降血糖的活性部位研究[J].世界中西医结合杂志,2015,10(9):1219-1221.
[12]吴娱明,邹宇晓,廖森泰,等.桑枝提取物对实验高血脂症小鼠的降血脂作用初步研究[J].2005,31(3):348-350.
[13]章丹丹,凌摇霜,张洪平,等.桑枝总黄酮体外抗炎活性及机制研究[J].时珍国医国药,2010,21(11):2787-2890.
[14]邬灏,卢笑丛,王有为.桑枝多糖分离纯化及其免疫作用的初步研究[J].武汉植物学研究,2005,23(1):81-84.