响应面法优化雪松松针中金丝桃苷的回流提取工艺
|
摘要
目的优化雪松松针中金丝桃苷的回流提取工艺。方法采用HPLC测定雪松松针中金丝桃苷的含量;基于单因素试验结果,以金丝桃苷含量为指标,根据Box-Behnken设计对影响回流提取的3个因素(料液比、乙醇浓度、提取时间)进行考察;通过响应面分析优化雪松松针中金丝桃苷的最佳回流提取工艺。结果优化得到的最佳回流提取工艺参数为乙醇浓度60%、料液比1︰16(g·mL~(-1))、回流提取1次,时间为100 min;在此工艺条件下提取得到的金丝桃苷平均含量为0.119 6 mg·g~(-1),与预测值0.119 4 mg·g~(-1)的相对偏差较小。结论优选得到的雪松松针中金丝桃苷回流提取工艺合理、可行。
OBJECTIVE To optimize the reflux extraction technology of hyperin in pine needle of Cedrus deodra. METHODS The content of hyperoside in pine needle of Cedrus deodara was determined by HPLC. On the basis of single factor test, the hyperoside content was used as indexes, three factors(solid-to-liquid ratio, ethanol concentration and extraction time) were studied by Box-Behnken design, the reflux extraction technology of hyperin in pine needle of Cedrus deodra was optimized by response surface methodology. RESULTS The optimal parameters of reflux extraction technology were as follows: ethanol concentration was 60%, solid-to-liquid ratio was 1:16(g·mL~(-1)), extraction time was 100 min for once. Under the above conditions, the average content of hyperin was 0.119 6 mg·g~(-1), which was similar to the predicted value 0.119 4 mg·g~(-1). CONCLUSION The preferred extraction technology of hyperin in pine needle of Cedrus deodra is reasonable and feasible.
OBJECTIVE To optimize the reflux extraction technology of hyperin in pine needle of Cedrus deodra. METHODS The content of hyperoside in pine needle of Cedrus deodara was determined by HPLC. On the basis of single factor test, the hyperoside content was used as indexes, three factors(solid-to-liquid ratio, ethanol concentration and extraction time) were studied by Box-Behnken design, the reflux extraction technology of hyperin in pine needle of Cedrus deodra was optimized by response surface methodology. RESULTS The optimal parameters of reflux extraction technology were as follows: ethanol concentration was 60%, solid-to-liquid ratio was 1:16(g·mL~(-1)), extraction time was 100 min for once. Under the above conditions, the average content of hyperin was 0.119 6 mg·g~(-1), which was similar to the predicted value 0.119 4 mg·g~(-1). CONCLUSION The preferred extraction technology of hyperin in pine needle of Cedrus deodra is reasonable and feasible.
引文
[1]ZHANG Z Q,XIAO P Y,LIU G M.Advances in studies on chemical constituents and pharmacological action of pine needle[J].Drugs Clin(现代药物与临床),2011,26(4):278-281.
[2]白朝辉,石晓峰,刘东彦,等.雪松松针的化学成分及药理作用研究概述[J].中国药师,2012,15(12):1791-1793.
[3]LIU D Y,SHI X F,LI C,et al.Chemical constituents of ethyl acetate fraction from pine needles of Cedrus deodara[J].Chin Tradit Herb Drugs(中草药),2011,42(10):1921-1924.
[4]LI J S,CHEN J H,MENG M J.Progress on the pharmacological effects and mechanism of hyperoside[J].J Guangdong Pharm Univ(广东药学院学报),2015,31(2):269-272.
[5]李敏芳,李慧,王学美.金丝桃苷药理作用研究进展[J].中国中医药信息杂志,2008,15(4):102-104.
[6]DU Y J,WANG Y M,WU H B,et al.Studies on optimal extraction technology of hyperoside from hawthorn through orthogonal experiments[J].China J Chin Med(中医学报),2015,30(4):565-567.
[7]CAI E B,XU J,ZHEN X M,et al.Study on composite enzyme assisted extraction of hyperoside from Acanthopanax sessiflorus[J].Herald Med(医药导报),2016,35(6):636-639.
[8]柯仲成,程子洋,陈龙.Box-Behnken响应面法优化贯叶金丝桃中金丝桃苷的提取工艺[J].中成药,2012,34(10):2023-2025.
[2]白朝辉,石晓峰,刘东彦,等.雪松松针的化学成分及药理作用研究概述[J].中国药师,2012,15(12):1791-1793.
[3]LIU D Y,SHI X F,LI C,et al.Chemical constituents of ethyl acetate fraction from pine needles of Cedrus deodara[J].Chin Tradit Herb Drugs(中草药),2011,42(10):1921-1924.
[4]LI J S,CHEN J H,MENG M J.Progress on the pharmacological effects and mechanism of hyperoside[J].J Guangdong Pharm Univ(广东药学院学报),2015,31(2):269-272.
[5]李敏芳,李慧,王学美.金丝桃苷药理作用研究进展[J].中国中医药信息杂志,2008,15(4):102-104.
[6]DU Y J,WANG Y M,WU H B,et al.Studies on optimal extraction technology of hyperoside from hawthorn through orthogonal experiments[J].China J Chin Med(中医学报),2015,30(4):565-567.
[7]CAI E B,XU J,ZHEN X M,et al.Study on composite enzyme assisted extraction of hyperoside from Acanthopanax sessiflorus[J].Herald Med(医药导报),2016,35(6):636-639.
[8]柯仲成,程子洋,陈龙.Box-Behnken响应面法优化贯叶金丝桃中金丝桃苷的提取工艺[J].中成药,2012,34(10):2023-2025.