光源、氮源和碳源对紫色非硫细菌生长特性的影响
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摘要
紫色非硫细菌(PNSB)能在厌氧光照条件下将污水中的氨氮、有机物和磷同化到细菌体内用于合成蛋白质等细胞物质,而不是转化为CO_2和N_2.为了优化PNSB的生长条件,以沼泽红假单胞菌为研究对象,考察了光源、氮源和碳源类型对PNSB生长的影响.结果表明厌氧红外光条件下PNSB的生长速率约是白炽灯条件下的3倍;PNSB对氨氮(NH_4~+-N)的利用速率最快,同时也可利用硝态氮(NO_3~--N),亚硝态氮(NO_2~--N);PNSB对乙酸钠的利用速率最快,其次是葡萄糖,最难利用的是淀粉,主要归因于大分子有机物需要水解酸化后才能被PNSB吸收利用.厌氧红外光条件下PNSB处理城市污水具有较好的应用前景.
Purple non-sulfur bacteria(PNSB) can assimilate ammonia, organic matter and phosphorus in wastewater into the cell body to composite proteins and other cellular substances under the condition of anaerobic illumination, instead of converting to CO_2 and N_2. In order to optimize PNSB growth conditions, Rhodopseudomonas palustris was used to study the effect of light, nitrogen and carbon on PNSB growth in this paper. The results showed that the PNSB growth rate under anaerobic infrared illumination conditions is approximately three times that of incandescent lamps. PNSB has the fastest utilization rate of ammonia(NH_4~+-N) and can utilize nitrate(NO_3~--N), nitrite(NO_2~--N). PNSB has the fastest utilization rate of sodium acetate, followed by glucose and starch. This is because macromolecular organic matter can only be further utilized by PNSB after being hydrolyzed and acidified. It is a promising to apply PNSB to wastewater treatment under anaerobic infrared illumination conditions.
Purple non-sulfur bacteria(PNSB) can assimilate ammonia, organic matter and phosphorus in wastewater into the cell body to composite proteins and other cellular substances under the condition of anaerobic illumination, instead of converting to CO_2 and N_2. In order to optimize PNSB growth conditions, Rhodopseudomonas palustris was used to study the effect of light, nitrogen and carbon on PNSB growth in this paper. The results showed that the PNSB growth rate under anaerobic infrared illumination conditions is approximately three times that of incandescent lamps. PNSB has the fastest utilization rate of ammonia(NH_4~+-N) and can utilize nitrate(NO_3~--N), nitrite(NO_2~--N). PNSB has the fastest utilization rate of sodium acetate, followed by glucose and starch. This is because macromolecular organic matter can only be further utilized by PNSB after being hydrolyzed and acidified. It is a promising to apply PNSB to wastewater treatment under anaerobic infrared illumination conditions.
引文
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[2]Kuo,F S,Chien Y H,Chen C J,Effects of light sources on growth and carotenoid content of photosynthetic bacteria Rhodopseudomonas palustris[J].Bioresource Technology,2012,113(4):315-318.
[3]Hülsen T,Batstone D J,Keller J.Phototrophic bacteria for nutrient recovery from domestic wastewater[J].Water Research,2014,50(3):18-26.
[4]樊凌雯,张肇铭.利用光合细菌处理糖蜜酒精发酵废液中试研究[J].中国环境科学,1998,18(2):173-175.Fan L X,Zhang Z M.A pilot test on the treatment of sugar-syrup alcohol ferment wastewater with photosynthetic bacteria[J].China Environmental Science,1998,18(2):173-175.
[5]Yang A,Zhang G,Meng F,et al.Membrane concentrate treatment by photosynthetic bacteria:Feasibility and tolerance mechanism analysis[J].Bioresource Technology,2018,253:378-381.
[6]董姗,张光明.不同条件对光合细菌沉降性的影响[J].工业安全与环保,2013,39(2):5-7.Dong S,Zhang G M.Effects of Different Factors on PSBSedimentation[J].Industrial Safety and Environmetal Protection,2013,39(2):5-7.
[7]Zhou Q,Zhang P,Zhang G,et al.Biomass and pigments production in photosynthetic bacteria wastewater treatment:Effects of photoperiod[J].Bioresource Technology,2014,190(179C):196-200.
[8]Qi X,Ren Y,Tian E,et al.The exploration of monochromatic nearinfrared LED improved anoxygenic photosynthetic bacteria Rhodopseudomonas sp.for wastewater treatment[J].Bioresource Technology,2017,241:620.
[9]曹林波,崔战利.节能灯替代白炽灯为光源对光合细菌SXY-8菌株生长及活细胞吸收光谱的影响[J].黑龙江八一农垦大学学报,2015,(2):74-78.Cao L B,Cui Z L.Effects of Illuminant with Energy-saving Lamps Replacing Incandescent Lamps on Growth and Absorption Spectra of Photosynthetic Bacteria SXY-8[J].Journal of Heilongjiang Bayi Agricultural University,2015,(2):74-78.
[10]李家洲,张冬青,黄荣林,等.近红外光源对光合细菌生长的影响[J].中国酿造,2012,31(5):114-117.Li J Z,Zhang D Q,Huang R L,et al.Influence of near-infrared on growth of photosynthetic bacteria[J].China Brewing,2012,31(5):114-117.
[11]安静.光源和光谱对光合产氢菌群产氢工艺影响研究[D].河南农业大学,2009.An J.Effect of Spectrum on Hydrogen Production by Photosynthetic Bacteria[D].Henan Agricultural University,2009.
[12]李圭白,张杰.水质工程学[M].2版.北京:中国建筑工业出版社,2013.Li G B,Zhang J.Water quality engineering[M].2nd Edition,Beijing:China Architecture and Building Press,2013.
[13]李晓玲,金晓弟,张小民.脱氮光合细菌的分离及其脱氮条件研究[J].山西大学学报,2002,25(4):350-353.Li X L,Jin X D,Zhang X M.Study on isolation of ednitrifing photosynthetic bacteria and their denitrifing conditions[J].Journal of Shanxi University,2002,25(4):350-353.
[14]蒋鹏.一株以亚硝氮为唯一氮源生长的不产氧光合细菌对无机三态氮的去除和相互转化[D].泉州:华侨大学,2014.Jiang P.Study on nitrogen transformation and removal of inorganic nitrogen by anoxygenic phototrophic bacteria grown on nitrite as sole nitrogen source[D].Quanzhou:Huaqiao University,2014.
[15]卢玉凤,张光明.苹果酸和微量元素改善光合细菌处理制糖废水[J].工业安全与环保,2013,39(2).Lu Y F,Zhang G M.Adding of Malic Acid and Trace Elements to Improve the Effect of Sugar Wastewater Treatment by Photosynthetic Bacteria[J].Industrial Safety and Environmetal Protection,2013,39(2).
[16]郑伟华.光合细菌(PSB)膜法工艺处理高浓度淀粉废水[D].兰州理工大学,2009.Zheng W H.Treament of High-concentration Starch Wastewater with Photosynthetic Bacteria(PSB)New-membrane Process[D].Lanzhou University of Technology,2009.
[17]Matassa S,Batstone D J,Hülsen T,et al.Can direct conversion of used nitrogen to new feed and protein help feed the world?[J].Environmental Science&Technology,2015,49(9):5247-54.
[18]Hülsen T,Hsieh K,Lu Y,et al.Simultaneous treatment and single cell protein production from agri-industrial wastewaters using purple phototrophic bacteria or microalgae-A comparison[J].Bioresource Technology,2018,254:214.