山西猪伪狂犬病免疫与野毒感染情况调研
|
摘要
山西2013年以来,即使使用gE基因缺失活疫苗免疫的猪场,也有母猪发生流产、仔猪疑似伪狂犬病病死情况,给养猪生产造成一定的经济损失。2016年、2017年、2018年项目组对山西不同地市的11个规模型种猪场调研证实它们使用的是正规厂家生产的猪伪狂犬病gE基因缺失疫苗,大部分猪场母猪采取普免,仔猪采取3次免疫;共检测11个猪场母猪血样388头份,野毒感染阳性率为57.94%,最高阳性率为83.33%;仔猪血样727头份,阳性率为34.66%,最高为70.00%,只有一个猪场仔猪没有检出野毒感染抗体。另外,从疑似伪狂犬病病死猪病料检测出猪伪狂犬病病毒阳性19份。初步掌握了山西猪伪狂犬病免疫与感染情况,为有效控制该病流行提供依据。
Some sows had miscarried and piglets died of suspected pseudorabies in the some of pig farms immunized with gE gene deletion vaccine since 2013, which had caused certain economic losses to the pig production.The 11 large-scale breeding pig livestock farms were investigated in different cities of Shanxi during 2016 years,2017 years and 2018 years, and it confirmed that sows were vaccinated and the piglets were immunized three times with pseudorabies gE gene deletion vaccine by the formal manufacturers in the most of pig farms. A total of 388 blood samples of sows were detected in 11 pig farms, and the positive rate of wild virus infection was 57.94%,even the highest positive rate was 83.33%.Meanwhile, there were 727 blood samples of piglets, and the positive rate was34.66%, the highest positive rate was 70.00%.Furthermore, the wild virus infection antibody of piglets was not detected only in one pig farm. In addition, 19 porcine pseudorabies virus positive samples were detected from suspected pseudorabies disease dead pigs.The immune and infection situation of pseudorabies virus was preliminarily mastered in Shanxi, which provided the basis for effective control of the disease.
Some sows had miscarried and piglets died of suspected pseudorabies in the some of pig farms immunized with gE gene deletion vaccine since 2013, which had caused certain economic losses to the pig production.The 11 large-scale breeding pig livestock farms were investigated in different cities of Shanxi during 2016 years,2017 years and 2018 years, and it confirmed that sows were vaccinated and the piglets were immunized three times with pseudorabies gE gene deletion vaccine by the formal manufacturers in the most of pig farms. A total of 388 blood samples of sows were detected in 11 pig farms, and the positive rate of wild virus infection was 57.94%,even the highest positive rate was 83.33%.Meanwhile, there were 727 blood samples of piglets, and the positive rate was34.66%, the highest positive rate was 70.00%.Furthermore, the wild virus infection antibody of piglets was not detected only in one pig farm. In addition, 19 porcine pseudorabies virus positive samples were detected from suspected pseudorabies disease dead pigs.The immune and infection situation of pseudorabies virus was preliminarily mastered in Shanxi, which provided the basis for effective control of the disease.
引文
[1]Cramer S D,Campbell G A,Njaa B L,et al.Pseudorabies virus infection in Oklahoma hunting dogs[J].Journal of Veterinary Diagnostic Investigation,2011,23(5):915-923.
[2]郭广富,曹军平,朱爱萍,等.猪伪狂犬病病毒泰州株的分离鉴定[J].江苏农业科学,2015,43(3):200-202.
[3]Pedersen K,Bevins S N,Baroch J A,et al.Pseudorabies in feral swine in the United States,2009-2012[J].Journal of Wildlife Diseases,2013,49(3):709-713.
[4]彭金美,安同庆,赵鸿远,等.猪伪狂犬病病毒新流行株的分离鉴定及抗原差异性分析[J].中国预防兽医学报,2013,35(1):1-4.
[5]Cao S,Chen H,Zhao J,et al.Detection of porcine circovirus type 2,porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR[J].Vet Res Commun,2005,29(3):263-269.
[6]Ye C,Zhang Q Z,Tong G Z,et al.Genomic characterization of emergent pseudorabies virus in Chinareveals marked sequence divergence:evidence for the existence of two major genotypes[J].Virology,2015,483:32-43.
[7]Wang C H,Yuan J,Qin H Y,et al.A novel g E-deleted pseudorabies virus(PRV)provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in china[J].Vaccine,2014,32(27):3379-3385.
[8]Gu Z,Hou C,Jiang P,et al.Emergence of highly virulent pseudorabies virus in southern China[J].Can J Vet Res,2015,79(3):221-228.
[9]雷宇平,宁艳,孙杰,等.山西省猪伪狂犬病野毒感染血清学调查[J].中国动物检疫,2015,32(9):1-3.
[2]郭广富,曹军平,朱爱萍,等.猪伪狂犬病病毒泰州株的分离鉴定[J].江苏农业科学,2015,43(3):200-202.
[3]Pedersen K,Bevins S N,Baroch J A,et al.Pseudorabies in feral swine in the United States,2009-2012[J].Journal of Wildlife Diseases,2013,49(3):709-713.
[4]彭金美,安同庆,赵鸿远,等.猪伪狂犬病病毒新流行株的分离鉴定及抗原差异性分析[J].中国预防兽医学报,2013,35(1):1-4.
[5]Cao S,Chen H,Zhao J,et al.Detection of porcine circovirus type 2,porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR[J].Vet Res Commun,2005,29(3):263-269.
[6]Ye C,Zhang Q Z,Tong G Z,et al.Genomic characterization of emergent pseudorabies virus in Chinareveals marked sequence divergence:evidence for the existence of two major genotypes[J].Virology,2015,483:32-43.
[7]Wang C H,Yuan J,Qin H Y,et al.A novel g E-deleted pseudorabies virus(PRV)provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in china[J].Vaccine,2014,32(27):3379-3385.
[8]Gu Z,Hou C,Jiang P,et al.Emergence of highly virulent pseudorabies virus in southern China[J].Can J Vet Res,2015,79(3):221-228.
[9]雷宇平,宁艳,孙杰,等.山西省猪伪狂犬病野毒感染血清学调查[J].中国动物检疫,2015,32(9):1-3.