山西省晋中市规模猪场母猪伪狂犬病群流行率及感染风险因素横断面研究
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摘要
为了解2018年山西省晋中市规模猪场母猪伪狂犬病血清学场群流行率,寻找猪伪狂犬病毒感染的潜在风险因素,以母猪存栏大于100头的75个规模猪场为研究目标,按照发现疫病的抽样策略,采用两阶段抽样方法,随机选取51个规模猪场,在每个养殖场随机抽取30份血清样品进行感染抗体检测,并对所有检测的规模猪场开展问卷调查。抗体检测结果显示,晋中市规模猪场母猪伪狂犬病场群流行率为40.90%(95%CI:27.3%～54.5%)。单因素分析显示,风险因素为"方圆3 km内有其他家畜养殖场、交易市场或者屠宰场",保护性因素为"引进猪只时检测伪狂犬病毒感染抗体和1年内引进公猪精液"。建立的模型拟合度较好,模型ROC曲线下面积为0.835(95%CI:0.658～1.000)。调查结果提示,新建养殖场要与其他的家畜养殖场、交易市场或者屠宰场保持安全的养殖距离,规模养殖场要坚持自繁自养,在引进种猪和公猪精液时一定要进行严格地PRV检测。
In order to recognize the sero-prevalence of pseudorabies virus(PRV)in sows at the farm or herd level in large-scale farms in Jinzhong City of Shanxi Province,and to identify the potential risk factors,taking 75 large-scale farms(the number of sows≥100)as the research objects,then two-stage random sampling strategy was adopted to choose 51 farms,and 30 serum samples were randomly selected from each farm for detection of PRV antibodies. Meanwhile,a questionnaire survey was carried out towards all selected large-scale farms. The results showed that the prevalence rate of PRV at the herd level in Jinzhong City was 40.90%(95% CI:27.3%–54.5%). Single factor analysis showed that the risk factor was"there were other livestock farms,trading markets or slaughterhouses within 3 km". The protective factor was"only the infection antibodies of PRV were carried out detection when pigs were introduced"and"semen of boars was introduced within 1 year". In addition,the established model had a good fit,and the area under the model ROC curve was 0.835(95% CI:0.658–1.000). The survey results suggested that the new farms should maintain a safe distance with other livestock farms,trading markets or slaughterhouses. Largescale farms should carry out animal production and reproduction by their own,and PRV detection should be strictly implemented when the breeding pigs and boar semen were introduced.
In order to recognize the sero-prevalence of pseudorabies virus(PRV)in sows at the farm or herd level in large-scale farms in Jinzhong City of Shanxi Province,and to identify the potential risk factors,taking 75 large-scale farms(the number of sows≥100)as the research objects,then two-stage random sampling strategy was adopted to choose 51 farms,and 30 serum samples were randomly selected from each farm for detection of PRV antibodies. Meanwhile,a questionnaire survey was carried out towards all selected large-scale farms. The results showed that the prevalence rate of PRV at the herd level in Jinzhong City was 40.90%(95% CI:27.3%–54.5%). Single factor analysis showed that the risk factor was"there were other livestock farms,trading markets or slaughterhouses within 3 km". The protective factor was"only the infection antibodies of PRV were carried out detection when pigs were introduced"and"semen of boars was introduced within 1 year". In addition,the established model had a good fit,and the area under the model ROC curve was 0.835(95% CI:0.658–1.000). The survey results suggested that the new farms should maintain a safe distance with other livestock farms,trading markets or slaughterhouses. Largescale farms should carry out animal production and reproduction by their own,and PRV detection should be strictly implemented when the breeding pigs and boar semen were introduced.
引文
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[6]KRAMER T,GRECO T M,LYNN W E,et al.Proteomic characterization of pseudorabies virus extracellular virions[J].Journal of virology,2011,85(13):6427-6441.
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[8]MOYNAGH O S,FODOR N,FODOR I.Indirect ELISAs based on recombinant and affinity-purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals[J].Act veterinaria hungarica,1999,47(1):37-50.
[9]邹敏,杨旭兵,郑辉,等.2012-2013年我国部分地区猪伪狂犬病流行病学调查[J].中国动物检疫,2015,32(4):1-5.
[2]李春华,王英,蒋凤英,等.猪伪狂犬病研究进展[J].动物医学进展,2008,29(3):68-72.
[3]STEGEMAN A.Aujeszky's disease(pseudorabies)virus eradication campaign in the Netherlands[J].Veterinary microbiology,1997,55:175-180.
[4]国务院办公厅.国务院办公厅关于印发《国家中长期动物疫病防治规划(2012-2020年)》的通知:国办发[2012]31号[A].北京:国务院,2012.
[5]雷宇平,宁艳,孙杰,等.山西省猪伪狂犬病野毒感染血清学调查[J].中国动物检疫,2015,32(9):1-3.
[6]KRAMER T,GRECO T M,LYNN W E,et al.Proteomic characterization of pseudorabies virus extracellular virions[J].Journal of virology,2011,85(13):6427-6441.
[7]沈朝建,王幼明.兽医流行病学调查与监测抽样技术手册[M].北京:中国农业出版社,2013.
[8]MOYNAGH O S,FODOR N,FODOR I.Indirect ELISAs based on recombinant and affinity-purified glycoprotein E of Aujeszky's disease virus to differentiate between vaccinated and infected animals[J].Act veterinaria hungarica,1999,47(1):37-50.
[9]邹敏,杨旭兵,郑辉,等.2012-2013年我国部分地区猪伪狂犬病流行病学调查[J].中国动物检疫,2015,32(4):1-5.