高效液相色谱法测定淫羊藿药材及饮片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量
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摘要
目的:采用高效液相色谱法,建立测定淫羊藿药材及饮片中朝藿定A、朝藿定B、朝藿定C和淫羊藿苷含量的方法。方法:采用Agilent ZORBAX SB-C_(18)柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.01%磷酸水(V∶V=24∶76),流速为1.0 ml/min,柱温为30℃,检测波长为270 nm。结果:朝藿定A、朝藿定B、朝藿定C和淫羊藿苷分别在0.068~0.408、0.316~1.896、0.280~2.240和0.434~2.604μg范围内线性关系良好,其平均加样回收率分别为103.49%、98.80%、96.75%和102.10%。结论:该方法快捷、准确,能够更好地实现对淫羊藿药材和饮片的质量控制。
OBJECTIVE: To establish the method for simultaneous determination of epimedins A,B and C and icariin in epimedii folium medicinal materials and decoction pieces by HPLC. METHODS: The samples were separated on an Agilent Zorbax SB-C_(18)( 4. 6 mm × 250 mm,5 μm) column,with mobile phase of 0. 01% acetonitrile with phosphoric acid water( V ∶ V = 24 ∶ 76); the flow rate was 1. 0 ml/min; the column temperature was 30 ℃; the wavelength was 270 nm. RESULTS: The linear ranges for epimedins A,B and C and icariin were respectively 0. 068-0. 408,0. 316-1. 896,0. 280-2. 240 and 0. 434-2. 604 μg,with average average recoveries of 103. 49%,98. 80%,96. 75% and 102. 10%. CONCLUSIONS: The established HPLC quantitative method is quick and accurate,and can be used to control the quality of epimedii folium medicinal materials and decoction pieces.
OBJECTIVE: To establish the method for simultaneous determination of epimedins A,B and C and icariin in epimedii folium medicinal materials and decoction pieces by HPLC. METHODS: The samples were separated on an Agilent Zorbax SB-C_(18)( 4. 6 mm × 250 mm,5 μm) column,with mobile phase of 0. 01% acetonitrile with phosphoric acid water( V ∶ V = 24 ∶ 76); the flow rate was 1. 0 ml/min; the column temperature was 30 ℃; the wavelength was 270 nm. RESULTS: The linear ranges for epimedins A,B and C and icariin were respectively 0. 068-0. 408,0. 316-1. 896,0. 280-2. 240 and 0. 434-2. 604 μg,with average average recoveries of 103. 49%,98. 80%,96. 75% and 102. 10%. CONCLUSIONS: The established HPLC quantitative method is quick and accurate,and can be used to control the quality of epimedii folium medicinal materials and decoction pieces.
引文
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[9]马清娟,王晶,韩凌,等.HPLC法同时测定淫羊藿药材中8种黄酮类成分的含量[J].沈阳药科大学学报,2014,31(12):970-978.
[10]黄弥娜,周燕妮,柳强,等.HPLC法测定不同产地淫羊藿中7种主要黄酮类成分的含量[J].第二军医大学学报,2015,36(12):1352-1355.
[2]孟宁,孔凯,李师翁.淫羊藿属植物化学成分及药理活性研究进展[J].西北植物学报,2010,30(5):1063-1073.
[3]李晓龙,刘虹宇,曹佩雪,等.HPLC同时测定21种淫羊藿中朝藿定C和淫羊藿苷的含量[J].药物分析杂志,2011,31(5):931-934.
[4]陈婷,许亮,杨燕云,等.HPLC法测定朝鲜淫羊藿及市售淫羊藿中淫羊藿苷等四种成分研究[J].中华中医药学刊,2014,32(7):1621-1623.
[5]雷永涛,梁妍,郝小燕,等.不同产地淫羊藿中4种活性成分含量的高效液相色谱法测定[J].时珍国医国药,2013,24(6):1404-1405.
[6]袁佳,钱冬女,滕俊.HPLC法测定不同产地淫羊藿饮片中淫羊藿苷的含量[J].海峡药学,2014,26(5):56-57.
[7]郭丽娜,马莹慧,赵伟,等.淫羊藿属植物中化学成分分析研究进展[J].中国新药杂志,2013,22(8):919-922,936.
[8]余晓晖,侯嘉,赵磊,等.甘肃不同产地淫羊藿中总黄酮和淫羊藿苷含量测定[J].甘肃中医学院学报,2014,31(1):13-16.
[9]马清娟,王晶,韩凌,等.HPLC法同时测定淫羊藿药材中8种黄酮类成分的含量[J].沈阳药科大学学报,2014,31(12):970-978.
[10]黄弥娜,周燕妮,柳强,等.HPLC法测定不同产地淫羊藿中7种主要黄酮类成分的含量[J].第二军医大学学报,2015,36(12):1352-1355.