不同时期摘叶对马瑟兰花色苷的影响
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摘要
以烟台蓬莱产区马瑟兰为试材,高效液相色谱(HPLC)法测定果皮花色苷含量,研究转色期前后摘叶处理对果皮花色苷种类和含量的影响。结果表明,转色期前摘叶能够显著增加花色苷的种类和含量(P<0.05),共检测到16种花色苷单体,其五类基本花色苷总量是对照的3.16倍;而转色后摘叶处理和对照组均检测出12种花色苷单体,五类基本花色苷总量只略高于对照组。
With Marselan in Penglai producing area of Yantai as raw material, the contents of anthocyanins in grape skin were determined by HPLC,and the effects of leaf picking before and after color conversion on the varieties and contents of anthocyanins in grape skin were studied. The results showed that the varieties and contents of anthocyanins could be increased significantly by picking leaves before color conversion(P<0.05), and 16 anthocyanins monomers were detected, and the total contents of the five basic anthocyanins were 3.16 times that of the control. 12 anthocyanins monomers were detected in both leaf picking group and control group, and the total amount of anthocyanins in five basic anthocyanins was only slightly higher than that in the control group.
With Marselan in Penglai producing area of Yantai as raw material, the contents of anthocyanins in grape skin were determined by HPLC,and the effects of leaf picking before and after color conversion on the varieties and contents of anthocyanins in grape skin were studied. The results showed that the varieties and contents of anthocyanins could be increased significantly by picking leaves before color conversion(P<0.05), and 16 anthocyanins monomers were detected, and the total contents of the five basic anthocyanins were 3.16 times that of the control. 12 anthocyanins monomers were detected in both leaf picking group and control group, and the total amount of anthocyanins in five basic anthocyanins was only slightly higher than that in the control group.
引文
[1]韩富亮,李杨,李记明,等.红葡萄酒花色苷结构和颜色的关系研究进展[J].食品与生物技术学报2011,30(3):328-336.
[2]邓军哲,屈慧鸽.葡萄花色素的研究概况[J].葡萄栽培与酿酒,1996(2):25-27.
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[4]张彦芳.河西走廊不同产地美乐(Merlot)果实成熟期间及原酒中花色苷成分比较[D].兰州:甘肃农业大学,2015.
[5]敬寒,温可睿,潘秋红,等.环境条件和栽培技术对葡萄花色苷生物合成的影响[J].中外葡萄与葡萄酒,2011(9):71-76.
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[9]张国涛,毛如志,陈绍林,等.香格里拉高海拔地区不同时间摘叶处理对“赤霞珠”葡萄果实品质的影响[J].北方园艺,2015(12):15-19.
[10]梁振昌.葡萄果皮花色苷构成特点、遗传规律及其在果实成熟过程中的变化[D].北京:中国科学院大学,2008.
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[12]QUAN Z,CHANG Q D,JUN W.Anthocyanins profile of grape berries of Vitis amurensis,its hybrids and their wines[J].Int J Mol Sci,2010,11(5):2212-2228.
[13]XING R R,LI S Y.Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in Cabernet Sauvignon(Vitis vinifera L.)grape berries[J].J Agr Food Chem,2015,63(12):3251-3260.
[14]AGEORGES A,FEMANDEZ L,VIALET S,et al.Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red color of grape berries[J].Plant Sci,2006,170(2):372-383.
[15]刘树文,何玲,任玉华.葡萄果实中花色素合成及其影响因素[J].中外葡萄与葡萄酒,1999(2):79-81.
[16]SIMS C,MORRIS J.A comparison of the color components and color stability of redwine from Noble and Cabernet Sauvignonat various p H levels[J].Am J Enol Viticult,1985,36(3):181-184.
[17]杨晓慧,陈为凯,何非,等.5个红色酿酒葡萄品种花色苷组成差异分析[J].西北农业学报,2017,26(11):1648-1654.
[18]邢婷婷,杨航宇,王雯染,等.14个欧亚种红色酿酒葡萄品种(品系)的花色苷组成和含量分析[J].果树学报,2018,35(2):147-157.
[19]李琪,李广,金丽琼,等.HPLC法测定甘肃地产不同品种酿酒葡萄中的花色苷[J].中国酿造,2014,33(3):132-136.
[20]PASTORE C,ZENONI S,FASOLIV M,et al.Selective defoliation affects plant growth,fruit transcriptional ripening program and flavonoid metabolism in grapevine[J].BMC Plant Biol,2013,13:30.
[21]DOWNEY M O,HARVEY J S,ROBINSON S P.The effect of bunch shading on berry development and flavonoid accumulation in Shiraz grapes[J].Aust J Grape Wine Res,2004,10:55-73.
[22]CHIRITI E,GUIDONI S,FETTANDINO A,et al.Effect of different cluster sunlight exposure levels on ripening and anthocyanin accumulation in Nebbiolo grapes[J].Am J Enol Vitic,2010,61:23-30.
[23]NEZ V,MONAGAS M,GOMEZ M C,et al.Vitis vinifera L.cv.grapes characterized by its anthocyanin profile[J].Postharvest Biol Tech,2004,31(1):69-79.
[24]JACKSON R S.Wine science principle and applications[M].Burlington:Academic Press,2008:287-295.
[25]MATSYAMA S,TANAZAWA F,KOBAYASHI H,et al.Leaf removal accelerated accumulation of delphinidin-based anthocyanins in"Muscat Bailey A"and Vitis vinifera(Muscat Hamburg)]grape skin[J].Japan Soc Hort Sci,2014,83:17-22.
[26]成果.微环境调控‘赤霞珠’葡萄果实花色苷代谢的研究[D].杨凌:西北农林科技大学,2015.
[27]于淼,刘海峰,王军.ABA对葡萄花色苷合成相关基因表达的影响[J].果树学报,2012,29(1):29-35.
[28]朱婷婷,梁东,夏惠.R2R3-MYB调控果实花色苷合成的研究进展[J].基因组学与应用生物学,2016,35(4):985-991.
[29]JOSE T M,LOYOLA R,VRGA A,et al.Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera[J].J Exp Bot,2009,60(3):859-867.
[2]邓军哲,屈慧鸽.葡萄花色素的研究概况[J].葡萄栽培与酿酒,1996(2):25-27.
[3]GUIDOIN S,FERRANDINO A,NOVELLO V.Effects of seasonal and aronomical practices on skin anthocyanin profile of Nebbiolo grapes[J].Am J Enol Viticult,2008,59(1):22-29.
[4]张彦芳.河西走廊不同产地美乐(Merlot)果实成熟期间及原酒中花色苷成分比较[D].兰州:甘肃农业大学,2015.
[5]敬寒,温可睿,潘秋红,等.环境条件和栽培技术对葡萄花色苷生物合成的影响[J].中外葡萄与葡萄酒,2011(9):71-76.
[6]艾丽丽,张振文.沙城产区主栽酿酒葡萄品质的研究[J].西北农业学报,2011,20(8):116-120.
[7]LEMUT M S,TROST K,SIVILOTTI P,et al.Pinot Noir grape colour related phenolics as affected by leaf removal treatments in the Vipava Valley[J].Food Compos Anal,2011,24:77.
[8]雷玉娟,王平,娄文浩,等.摘叶处理对赤霞珠葡萄果实品质的影响[J].中外葡萄与葡萄酒,2014(2):23-25.
[9]张国涛,毛如志,陈绍林,等.香格里拉高海拔地区不同时间摘叶处理对“赤霞珠”葡萄果实品质的影响[J].北方园艺,2015(12):15-19.
[10]梁振昌.葡萄果皮花色苷构成特点、遗传规律及其在果实成熟过程中的变化[D].北京:中国科学院大学,2008.
[11]WULFL W,NAGEL C.High-pressure liquid chromatographic separation of anthocyanin of Vitis vinifera[J].Am J Enol Viticult,1978,29(1):42-49.
[12]QUAN Z,CHANG Q D,JUN W.Anthocyanins profile of grape berries of Vitis amurensis,its hybrids and their wines[J].Int J Mol Sci,2010,11(5):2212-2228.
[13]XING R R,LI S Y.Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in Cabernet Sauvignon(Vitis vinifera L.)grape berries[J].J Agr Food Chem,2015,63(12):3251-3260.
[14]AGEORGES A,FEMANDEZ L,VIALET S,et al.Four specific isogenes of the anthocyanin metabolic pathway are systematically co-expressed with the red color of grape berries[J].Plant Sci,2006,170(2):372-383.
[15]刘树文,何玲,任玉华.葡萄果实中花色素合成及其影响因素[J].中外葡萄与葡萄酒,1999(2):79-81.
[16]SIMS C,MORRIS J.A comparison of the color components and color stability of redwine from Noble and Cabernet Sauvignonat various p H levels[J].Am J Enol Viticult,1985,36(3):181-184.
[17]杨晓慧,陈为凯,何非,等.5个红色酿酒葡萄品种花色苷组成差异分析[J].西北农业学报,2017,26(11):1648-1654.
[18]邢婷婷,杨航宇,王雯染,等.14个欧亚种红色酿酒葡萄品种(品系)的花色苷组成和含量分析[J].果树学报,2018,35(2):147-157.
[19]李琪,李广,金丽琼,等.HPLC法测定甘肃地产不同品种酿酒葡萄中的花色苷[J].中国酿造,2014,33(3):132-136.
[20]PASTORE C,ZENONI S,FASOLIV M,et al.Selective defoliation affects plant growth,fruit transcriptional ripening program and flavonoid metabolism in grapevine[J].BMC Plant Biol,2013,13:30.
[21]DOWNEY M O,HARVEY J S,ROBINSON S P.The effect of bunch shading on berry development and flavonoid accumulation in Shiraz grapes[J].Aust J Grape Wine Res,2004,10:55-73.
[22]CHIRITI E,GUIDONI S,FETTANDINO A,et al.Effect of different cluster sunlight exposure levels on ripening and anthocyanin accumulation in Nebbiolo grapes[J].Am J Enol Vitic,2010,61:23-30.
[23]NEZ V,MONAGAS M,GOMEZ M C,et al.Vitis vinifera L.cv.grapes characterized by its anthocyanin profile[J].Postharvest Biol Tech,2004,31(1):69-79.
[24]JACKSON R S.Wine science principle and applications[M].Burlington:Academic Press,2008:287-295.
[25]MATSYAMA S,TANAZAWA F,KOBAYASHI H,et al.Leaf removal accelerated accumulation of delphinidin-based anthocyanins in"Muscat Bailey A"and Vitis vinifera(Muscat Hamburg)]grape skin[J].Japan Soc Hort Sci,2014,83:17-22.
[26]成果.微环境调控‘赤霞珠’葡萄果实花色苷代谢的研究[D].杨凌:西北农林科技大学,2015.
[27]于淼,刘海峰,王军.ABA对葡萄花色苷合成相关基因表达的影响[J].果树学报,2012,29(1):29-35.
[28]朱婷婷,梁东,夏惠.R2R3-MYB调控果实花色苷合成的研究进展[J].基因组学与应用生物学,2016,35(4):985-991.
[29]JOSE T M,LOYOLA R,VRGA A,et al.Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera[J].J Exp Bot,2009,60(3):859-867.