长爪沙鼠Eef1a2基因的克隆与同源性分析
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摘要
目的长爪沙鼠真核蛋白延长因子1a2(eukaryotic translation elongation factor 1 alpha 2,Eef1a2)基因的克隆,测序和同源性分析。方法提取长爪沙鼠骨骼肌组织的mRNA,通过同源扩增和cDNA末端快速扩增技术(rapid amplification of cDNA ends,RACE)克隆Eef1a2 cDNA序列全长。通过DANSTAR软件比对长爪沙鼠和人类、大鼠、小鼠Eef1a2的cDNA和氨基酸序列,并分析其同源性。结果长爪沙鼠Eef1a2 cDNA序列全长1812 bp,编码区(coding sequence,CDs)1392 bp,编码463个氨基酸。其核苷酸序列与人类、大鼠和小鼠的同源性分别为88.6%,94.0%和93.8%;长爪沙鼠Eef1a2氨基酸序列与人类、大鼠和小鼠的同源性分别为100%,99.9%和99.9%。结论长爪沙鼠Eef1a2的核苷酸序列和氨基酸序列均与人、大鼠、小鼠高度同源。因此,长爪沙鼠可能是一种人类Eef1a2基因研究的理想模型。
Objective The purpose of the present studies was to clone and sequence eukaryotic protein elongation factor 1a2( Eef1a2) gene of Mongolian gerbils,and analyze homology of Eef1a2 between four species. Method The Eef1a2 c DNA was cloned from skeletal muscle mRNA of Mongolian gerbils by homologous amplification and rapid amplification of c DNA ends( RACE). Then the homology of nucleotide and amino acid sequences of Eef1a2 were analyzed among gerbils,human,rats and mice by DANSTAR software. Result The c DNA length of Mongolian gerbils Eef1a2 was 1812 bp,coding sequence( CDs) 1392 bp,coding 463 amino acids. It exhibited88. 6% identity with human Eef1a2,94. 0% identity with rat Eef1a2,and 93. 8% identity with mouse Eef1a2.And Eef1a2 of Mongolian gerbils had 100%,99. 9% and 99. 9% amino acid sequence identity to human,rats and mice,respectively. Conclusion Both nucleotide and amino acid sequences of Eef1a2 from Mongolian gerbils are high identity with human Eef1a2. Therefore,Mongolian gerbils are expected to be one of the ideal models for studies of human Eef1a2.
Objective The purpose of the present studies was to clone and sequence eukaryotic protein elongation factor 1a2( Eef1a2) gene of Mongolian gerbils,and analyze homology of Eef1a2 between four species. Method The Eef1a2 c DNA was cloned from skeletal muscle mRNA of Mongolian gerbils by homologous amplification and rapid amplification of c DNA ends( RACE). Then the homology of nucleotide and amino acid sequences of Eef1a2 were analyzed among gerbils,human,rats and mice by DANSTAR software. Result The c DNA length of Mongolian gerbils Eef1a2 was 1812 bp,coding sequence( CDs) 1392 bp,coding 463 amino acids. It exhibited88. 6% identity with human Eef1a2,94. 0% identity with rat Eef1a2,and 93. 8% identity with mouse Eef1a2.And Eef1a2 of Mongolian gerbils had 100%,99. 9% and 99. 9% amino acid sequence identity to human,rats and mice,respectively. Conclusion Both nucleotide and amino acid sequences of Eef1a2 from Mongolian gerbils are high identity with human Eef1a2. Therefore,Mongolian gerbils are expected to be one of the ideal models for studies of human Eef1a2.
引文
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[15]崔美芝,刘恩重,柳英侠.长爪沙鼠在医学研究中的应用[J].哈尔滨医科大学学报,1996,(2):205-206.
[2]丁贤明,钱宝珍.长爪沙鼠在生物医学中的应用及其生理生化研究进展[J].中国比较医学杂志,2006,(5):313-318.
[3]李长龙,杜小燕,陈振文.长爪沙鼠资源开发利用进展[J].中国实验动物学报,2014,(6):106-109.
[4]Li X,Lu J,Wang Y,et al.Establishment and Characterization of a Newly Established Diabetic Gerbil Line[J].PLo S One,2016,11(7):e159420.
[5]Soares D C,Abbott C M.Highly homologous e EF1A1 and e EF1A2 exhibit differential post-translational modification with significant enrichment around localised sites of sequence variation[J].Biol Direct,2013,8(1):29.
[6]Amiri A,Noei F,Jeganathan S,et al.e EF1A2 activates Akt and stimulates Akt-dependent actin remodeling,invasion and migration[J].Oncogene,2007,26(21):3027-3040.
[7]Pellegrino R,Calvisi D F,Neumann O,et al.EEF1A2inactivates p53 by way of PI3K/AKT/m TOR-dependent stabilization of MDM4 in hepatocellular carcinoma[J].Hepatology,2014,59(5):1886-1899.
[8]Li Z,Qi C F,Shin D M,et al.Eef1a2 promotes cell growth,inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas[J].PLo S One,2010,5(5):e10755.
[9]Xu C,Hu D M,Zhu Q.e EF1A2 promotes cell migration,invasion and metastasis in pancreatic cancer by upregulating MMP-9 expression through Akt activation[J].Clin Exp Metastasis,2013,30(7):933-944.
[10]Doig J,Griffiths L A,Peberdy D,et al.In vivo characterization of the role of tissue-specific translation elongation factor 1A2 in protein synthesis reveals insights into muscle atrophy[J].FEBS J,2013,280(24):6528-6540.
[11]Anand N,Murthy S,Amann G,et al.Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer[J].Nat Genet,2002,31(3):301-305.
[12]Klupa T,Malecki M T,Pezzolesi M,et al.Further evidence for a susceptibility locus for type 2 diabetes on chromosome 20q13.1-q13.2[J].Diabetes,2000,49(12):2212-2216.
[13]Ghosh S,Watanabe R M,Hauser E R,et al.Type 2 diabetes:evidence for linkage on chromosome 20 in 716 Finnish affected sib pairs[J].Proc Natl Acad Sci U S A,1999,96(5):2198-2203.
[14]张宏利,朱鋐达,骆天红,等.染色体20q13区域SNP与中国人2型糖尿病的相关性[J].上海第二医科大学学报,2005,(7):653-656.
[15]崔美芝,刘恩重,柳英侠.长爪沙鼠在医学研究中的应用[J].哈尔滨医科大学学报,1996,(2):205-206.