Mylpf基因在自发性糖尿病长爪沙鼠6种组织中的表达水平分析
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摘要
目的分析Mylpf基因在糖尿病长爪沙鼠6种组织中的表达水平,进而探究长爪沙鼠糖尿病发生的分子机制。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,使用Real-time PCR和Western blotting技术分别检测动物的骨骼肌、肝脏、脂肪、肾脏、心脏和脑组织中Mylpf的mRNA和蛋白表达水平。结果 Real-time PCR的结果表明,在骨骼肌、心脏和脑组织中,Mylpf在糖尿病组mRNA表达水平高于对照组。在脂肪组织和肝脏组织中糖尿病组mRNA表达水平低于对照组。在肾脏组织中两组mRNA表达水平基本相同。Western blotting结果显示,在骨骼肌中Mylpf在糖尿病组蛋白表达水平高于对照组,且有统计学差异,在心脏和脂肪组织中Mylpf在糖尿病组蛋白表达水平低于对照组,在肾脏组织中蛋白表达水平基本相同,在肝脏和脑组织中没有检测出。结论 Mylpf与长爪沙鼠糖尿病的发生有一定相关性,该基因对长爪沙鼠糖尿病的影响可能主要发生在骨骼肌和心脏组织中。
Objective To analysis of Mylpf expression levels in six tissues of diabetic Mongolian gerbils and to explore the molecular mechanism of diabetes in Mongolian gerbils.Method We selected a diabetic group and a control group,each group has 6 Mongolian gerbils.Mylpf mRNA and protein expression level in skeletal muscle,liver,adipose,kidney,heart and brain were separately detected using Real-time PCR and Western blotting.Result The Real-time PCR showed that compared with control animal,mRNA expression of Mylpfin skeletal muscle,heart and brain was increased in diabetic group.The relative expression level in diabetic group exhibited decreased compared with control in adipose and liver.And there were no differences between two groups in the kidney.The Western blotting result display that protein of Mylpf in skeletal muscle was significantly increased in diabetes group.The expression of Mylpf in diabetic Mongolian gerbils heart and adipose was lower than control group.Mylpf protein was almost undetectable in liver and brain tissue.And Varied expression was observed in kidney,but there is no difference.Conclusion There is a correlation between Mylpf and the occurrence of diabetes in Mongolian gerbils and theresult show that the effect of Mylpf mainly occurred in skeletal muscle and heart tissue of diabetic gerbils.
Objective To analysis of Mylpf expression levels in six tissues of diabetic Mongolian gerbils and to explore the molecular mechanism of diabetes in Mongolian gerbils.Method We selected a diabetic group and a control group,each group has 6 Mongolian gerbils.Mylpf mRNA and protein expression level in skeletal muscle,liver,adipose,kidney,heart and brain were separately detected using Real-time PCR and Western blotting.Result The Real-time PCR showed that compared with control animal,mRNA expression of Mylpfin skeletal muscle,heart and brain was increased in diabetic group.The relative expression level in diabetic group exhibited decreased compared with control in adipose and liver.And there were no differences between two groups in the kidney.The Western blotting result display that protein of Mylpf in skeletal muscle was significantly increased in diabetes group.The expression of Mylpf in diabetic Mongolian gerbils heart and adipose was lower than control group.Mylpf protein was almost undetectable in liver and brain tissue.And Varied expression was observed in kidney,but there is no difference.Conclusion There is a correlation between Mylpf and the occurrence of diabetes in Mongolian gerbils and theresult show that the effect of Mylpf mainly occurred in skeletal muscle and heart tissue of diabetic gerbils.
引文
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[21]Gaber E M,Jayaprakash P,Qureshi M A,et al.Effects of a sucrose-enriched diet on the pattern of gene expression,contraction and Ca(2+)transport in Goto-Kakizaki type 2diabetic rat heart[J].Exp Physiol,2014,99(6):881-893.
[22]De Sousa A R,Penalva L O,Marcotte E M,et al.Global signatures of protein and mRNA expression levels[J].Mol Biosyst,2009,5(12):1512-1526.
[2]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J].中华内分泌代谢杂志,2014,30(10):893-942.
[3]Li X,Lu J,Wang Y,et al.Establishment and Characterization of a Newly Established Diabetic Gerbil Line[J].PLo S One,2016,11(7):e159420.
[4]马珊珊,张爱琴,娄晓璐,等.2型糖尿病及其并发症的治疗进展[J].中国医药指南,2011,(33):269-271.
[5]汪会琴,胡如英,武海滨,等.2型糖尿病报告发病率研究进展[J].浙江预防医学,2016,(1):37-39.
[6]朱超,朱莹莹.Ⅱ型糖尿病动物模型的构建[J].中国实验动物学报,2013,(2):84-88.
[7]孙洁,杨兵全,孙子林.自噬与糖尿病骨骼肌病变[J].国际内分泌代谢杂志,2014,34(3):194-196.
[8]李亚芯,徐晨阳,张东慧,等.血管紧张素转移酶2(ACE2)在糖尿病致大鼠肝脏氧化应激损伤中的作用及机制分析[J].食品科学,2013,(7):279-283.
[9]陈小瑜,杨艾,李婷,等.2型糖尿病患者并发肾脏疾病影响因素的Meta分析[J].中国全科医学,2015,(22):2732-2736.
[10]魏平,梁瑞凯,谭明红,等.2型糖尿病肾病大鼠肾脏MCP-1与氧化应激相关性研究[J].第三军医大学学报,2011,(19):2021-2024.
[11]朱洪竹,阮晓娟,朱梅菊,等.运动训练对2型糖尿病大鼠肾脏NF-κB-p65和相关蛋白表达的影响及机制研究[J].体育科学,2016,(8):1-7.
[12]廖明松,李树森,王先银,等.2型糖尿病兔模型心脏结构和功能改变超声研究[J].四川医学,2004,(7):737-738.
[13]严婷,李玲玲,王怀明,等.2型糖尿病患者脂肪细胞因子与代谢综合征的关系[J].南方医科大学学报,2014,(2):275-278.
[14]王宪玲,贾建平,左萍萍,等.自发性2型糖尿病小鼠脑组织胆碱能神经递质系统的研究[J].首都医科大学学报,2006,(3):355-357.
[15]王丛阳,王辉,沈传陆.肌球蛋白轻链的调节及其对肿瘤细胞增殖、转移的影响[J].医学综述,2010(21):3251-3254.
[16]焦东东,蔡辉.肌球蛋白轻链在血管平滑肌舒缩中的机制研究进展[J].中西医结合心脑血管病杂志,2009,(12):1452-1454.
[17]Xu H,Xu G,Wang D,et al.Molecular cloning,sequence identification and expression analysis of novel caprine MYLPF gene[J].Mol Biol Rep,2013,40(3):2565-2572.
[18]Wang H L,Wang H,Zhu Z M,et al.Subcellular localization,expression patterns,SNPs and association analyses of the porcine HUMMLC2B gene[J].Mol Genet Genomics,2006,276(3):264-272.
[19]王军,邓昌彦,熊远著,等.猪HUMMLC2B基因第一内含子单核苷酸多态与胴体、肉质性状的相关分析[J].农业生物技术学报,2008,(5):764-769.
[20]Mc Laughlin T,Craig C,Liu L F,et al.Adipose Cell Size and Regional Fat Deposition as Predictors of Metabolic Response to Overfeeding in Insulin-Resistant and Insulin-Sensitive Humans[J].Diabetes,2016,65(5):1245-1254.
[21]Gaber E M,Jayaprakash P,Qureshi M A,et al.Effects of a sucrose-enriched diet on the pattern of gene expression,contraction and Ca(2+)transport in Goto-Kakizaki type 2diabetic rat heart[J].Exp Physiol,2014,99(6):881-893.
[22]De Sousa A R,Penalva L O,Marcotte E M,et al.Global signatures of protein and mRNA expression levels[J].Mol Biosyst,2009,5(12):1512-1526.