自发性糖尿病长爪沙鼠环氧化酶(COX)-2在三种组织中的表达
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摘要
目的研究环氧化酶(COX)-2基因在糖尿病长爪沙鼠3种组织中的表达水平,进一步探索其与长爪沙鼠糖尿病发生和发展的关系。方法选取糖尿病长爪沙鼠和正常沙鼠各6只,分别采集动物的骨骼肌、肝脏和肾脏组织,用实时PCR和Western blotting检测COX-2基因在各组织中mRNA和蛋白表达水平。结果实时PCR的结果表明,COX-2基因的mRNA水平在糖尿病组动物的骨骼肌组织中表达与对照组无明显差异,而肝脏和肾脏组织中有表达升高趋势。Western blotting结果显示,糖尿病组COX-2的蛋白水平在肝脏和肾脏组织中存在表达升高趋势,且肾脏具有统计学意义。结论 COX-2在糖尿病长爪沙鼠肝脏和肾脏组织中表达升高,在肾脏组织尤其明显,说明COX-2对长爪沙鼠糖尿病的影响可能发生在肝脏和肾脏组织中。
Objective To analyze Cyclooxygenase 2(COX-2) expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils.Methods Different tissues including skeletal muscle,liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively.COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting.Results The Real-time PCR showed that compared with control animal,the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney.As the results of Western blotting,the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group.The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney.And there was statistical significance in the kidney.Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils.It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.
Objective To analyze Cyclooxygenase 2(COX-2) expression level in different tissues of diabetic gerbils and to explore the relationship of diabetes occurrence and development in Mongolian gerbils.Methods Different tissues including skeletal muscle,liver and kidney were collected from 6 diabetic gerbils and 6 control animals respectively.COX-2 mRNA and protein expression level in different tissues were separately detected by Real-time PCR and Western blotting.Results The Real-time PCR showed that compared with control animal,the mRNA expression of COX-2 in skeletal muscle had no obvious difference whereas it exhibited uptrend in liver and kidney.As the results of Western blotting,the difference of COX-2 in skeletal muscle was almost undetectable in diabetes group.The relative expression level in diabetic group exhibited uptrend compared with control in liver and kidney.And there was statistical significance in the kidney.Conclusions COX-2 was high expressed in liver and more remarkably in kidney of diabetic gerbils.It may illustrate that the effect of COX-2 mainly occurred in liver and kidney of diabetic gerbils.
引文
[1]姜振伟,唐红梅.糖化血红蛋白和血清胱抑素C联合检测在糖尿病早期肾损伤中的应用价值[J].国际检验医学杂志,2012,33(3):759-760.
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[13]徐春.器官移植术后糖尿病的发病机制与防治措施[J].武警医学,2014,25(8):757-760.
[14]肖遥,武英,赵丹丹,等.高血压合并糖尿病发病机制研究进展[J].医学信息,2013,5(14):617.
[15]谢平,黄坚.2型糖尿病危险因素的研究进展[J].应用预防医学,2016,22(1):88-91.
[16]李聪然,游雪甫,蒋建东.糖尿病动物模型及研究进展[J].中国比较医学杂志,2005,15(1):59-63.
[17]赵玉琼.糖尿病动物模型[J].实验动物科学,2012,29(2):53-57.
[18]Jin W,Patti ME.Genetic determinants and molecular pathways in the pathogenesis of Type 2 diabetes[J].Clin Sci(Lond),2009,116(2):99-111.
[19]廖群,陈芳华.尿蛋白、微量白蛋白及尿蛋白电泳联合检测诊断糖尿病早期肾损害[J].中国实验诊断学,2011,15(11):1944-1945.
[20]谢席胜,艾娜,王宝福,等.糖尿病肾病流行病学研究进展[J].中国中西医结合肾病杂志,2013,14(10):937-940.
[21]李丽晶,刘国平,于磊.SGLT2抑制剂治疗2型糖尿病的研究进展[J].中国中西医结合肾病杂志,2016,17(1):82-85.
[2]American Diabetes Association.Diagnosis and classification of diabetes mellitus[J].Diabetes Care,2012,27(Suppl 1):S5-S10.
[3]赵永梅,李敬华,李贺.外源性胰岛素对2型糖尿病内皮功能影响的研究进展[J].现代中西医结合杂志,2016,(4):448-450.
[4]Ashcroft FM,Rorsman P.Diabetes mellitus and the p-cell:the last ten years[J].Cell,2012,148(6):1160-1171.
[5]赵家俭.环氧化酶-2与1型糖尿病[J].国外医学:内分泌学分册,2003,23(5):297-299.
[6]Sjholm A,Nystrm T.Endothelial in flammation in insulin resistance[J].Lancet,2005,365(9459):610-612.
[7]BeloquiO,Paramo JA,Orbe J,etal.Monocyte cyclooxygenase-2 overactivity:a new marker of subclinical atheroscleros is in asymptomatic subjects with cardiovascular risk factors[J].Eur Heart J,2005,26(2):153-158.
[8]李玲,吴开春,樊代明.环氧化酶-2与肿瘤[J].陕西肿瘤医学,2000,8(2):116-118.
[9]Robertson RP.Dominance of cyclooxygenase-2 in the regulation of pancreatic islet prostaglandin synthesis[J],Diabetes,1998,47(9):1379-1383.
[10]LuoC,KallajokiM,Gross R,et al.Cellular distribution and contribution of cyclooxygense-2 to diabetogenesis in NOD mouse[J].Cell Tissuse Res,2002,310(2):169-175.
[11]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013年版)[J]中华糖尿病杂志,2014,22(7):447-498.
[12]王敏.糖尿病发病机制的研究进展[J].铜陵职业技术学院学报,2016,15(1):44-47.
[13]徐春.器官移植术后糖尿病的发病机制与防治措施[J].武警医学,2014,25(8):757-760.
[14]肖遥,武英,赵丹丹,等.高血压合并糖尿病发病机制研究进展[J].医学信息,2013,5(14):617.
[15]谢平,黄坚.2型糖尿病危险因素的研究进展[J].应用预防医学,2016,22(1):88-91.
[16]李聪然,游雪甫,蒋建东.糖尿病动物模型及研究进展[J].中国比较医学杂志,2005,15(1):59-63.
[17]赵玉琼.糖尿病动物模型[J].实验动物科学,2012,29(2):53-57.
[18]Jin W,Patti ME.Genetic determinants and molecular pathways in the pathogenesis of Type 2 diabetes[J].Clin Sci(Lond),2009,116(2):99-111.
[19]廖群,陈芳华.尿蛋白、微量白蛋白及尿蛋白电泳联合检测诊断糖尿病早期肾损害[J].中国实验诊断学,2011,15(11):1944-1945.
[20]谢席胜,艾娜,王宝福,等.糖尿病肾病流行病学研究进展[J].中国中西医结合肾病杂志,2013,14(10):937-940.
[21]李丽晶,刘国平,于磊.SGLT2抑制剂治疗2型糖尿病的研究进展[J].中国中西医结合肾病杂志,2016,17(1):82-85.