基于UPLC-MS的沙米代谢组学分析
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摘要
目的:研究沙米地上部分不同组织的代谢物,分析不同组织的差异标志代谢物,挖掘其有效药用活性成分。方法:采用基于UPLC-MS非靶向代谢组学技术对沙米地上组织的药用活性成分进行分析,利用三重四级杆质谱的多反应监测模式(MRM)进行代谢物定量分析,多元统计方法分析沙米地上不同组织差异代谢物。结果:通过代谢组分析共检测到沙米地上组织中的506种代谢物,其中具有药用活性的成分包括黄酮类、生物碱类、多酚类、维生素、萜类、氨基酸等,大部分活性物质在沙米地上组织中含量明显富集,部分代谢物在叶、茎、穗中含量分布存在差异,穗中氨基酸含量显著,叶、茎中黄酮类、多酚类、生物碱类、维生素等次生代谢物含量显著。结论:沙米具有潜在的抗氧化、抗肿瘤、抗炎、抗菌、降血压、降血脂和保护心脑血管等功能。该研究不仅支持利用沙米地上组织开发新药极具前景,同时还为沙米作为新型中草药资源提供重要数据,推动我国干旱区、半干旱区可持续生态农业和健康产业的发展。
Objective: To evaluate the potential medicine usage,we aimed to verifying its medicinal active ingredients of Agriophyllum squarrosum. Method: We explored medicinal active ingredients in the aboveground tissues of A. squarrosum based on UPLC-MS non-targeted metabonomics analysis. Quantitative analysis of metabolites was carried out using a multi-reaction monitoring model(MRM) of triple quadrupole mass spectrometry. The multivariate statistical analysis was used to analyze the difference metabolites in the aboveground tissues of A. squarrosum. Result: With metabonomics analysis,a total of 506 metabolites were detected in the above-ground tissues of A. squarrosum,including flavonoids, alkaloids, polyphenols, vitamins, terpenes,amino acids, etc. Most of the active ingredients had a significant concentration in the aerial part of A. squarrosum,though some metabolites concentration differed among tissues. The spikes mainly contained amino acids,while the stems and leaves were rich in the secondary metabolites, including flavonoids, polyphenols,alkaloids,vitamins,etc. Conclusion: A. squarrosum has a variety of potential functions,such as anti-oxidation,anti-tumor, anti-inflammatory, antibacterial, lowering blood pressure, protecting cardiovascular and cerebrovascular,etc. This work supports that it is highly promising to develop new drugs using the above-ground tissues of A. squarrosum. Meanwhile,it also provides important data for A. squarrosum as a new Chinese herbal medicine resource which can play an important role in sustainable ecological agriculture and health industry in arid and semi-arid regions.
Objective: To evaluate the potential medicine usage,we aimed to verifying its medicinal active ingredients of Agriophyllum squarrosum. Method: We explored medicinal active ingredients in the aboveground tissues of A. squarrosum based on UPLC-MS non-targeted metabonomics analysis. Quantitative analysis of metabolites was carried out using a multi-reaction monitoring model(MRM) of triple quadrupole mass spectrometry. The multivariate statistical analysis was used to analyze the difference metabolites in the aboveground tissues of A. squarrosum. Result: With metabonomics analysis,a total of 506 metabolites were detected in the above-ground tissues of A. squarrosum,including flavonoids, alkaloids, polyphenols, vitamins, terpenes,amino acids, etc. Most of the active ingredients had a significant concentration in the aerial part of A. squarrosum,though some metabolites concentration differed among tissues. The spikes mainly contained amino acids,while the stems and leaves were rich in the secondary metabolites, including flavonoids, polyphenols,alkaloids,vitamins,etc. Conclusion: A. squarrosum has a variety of potential functions,such as anti-oxidation,anti-tumor, anti-inflammatory, antibacterial, lowering blood pressure, protecting cardiovascular and cerebrovascular,etc. This work supports that it is highly promising to develop new drugs using the above-ground tissues of A. squarrosum. Meanwhile,it also provides important data for A. squarrosum as a new Chinese herbal medicine resource which can play an important role in sustainable ecological agriculture and health industry in arid and semi-arid regions.
引文
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[31]Tsai Y M,CHONG I W,Hung J Y,et al.Syringetin suppresses osteoclastogenesis mediated by osteoblasts in human lung adenocarcinoma[J].Oncol Rep,2015,34(2):617-626.
[32]代菊英.氨茶碱药理作用与临床应用[J].中国现代药物应用,2009,3(2):120-121.
[33]方莲花,吕扬,杜冠华.秦皮的药理作用研究进展[J].中国中药杂志,2008,33(23):2732-2736.
[34]韩向东.固沙先锋植物沙米的生物学特征研究[J].甘肃林业科技,2007,32(4):3-8.
[35]强永在,巴俊杰,渠弼,等.蒙药材沙蓬的生药学研究[J].时珍国医国药,2009,20(1):45-46.
[36]CHEN G,ZHAO J,ZHAO X,et al.A psammophyte Agriophyllum squarrosum(L.)Moq.:a potential food crop[J].Genet Resour Crop Ev,2014,61(3):669-676.
[37]任文明,刘雪峰,倪春梅.毛乌素沙漠天然沙米营养成分的分析[J].内蒙古农业大学学报:自然科学版,2005,26(2):88-90.
[38]QIAN C,YIN H,SHI Y,et al.Population dynamics of Agriophyllum squarrosum,a pioneer annual plant endemic to mobile sand dunes,in response to global climate change[J].Sci Rep,2016,doi:10.1038/srep26613.
[39]尹成亮,赵杰才,胡进玲,等.环境异质性对潜在粮食作物沙米表型变异的影响[J].中国科学:生命科学,2016,46(11):1324-1335.
[2]罗布桑.蒙药学[M].呼和浩特:内蒙古人民出版社,2006:214.
[3]朱亚民.内蒙古植物药志.第一卷[M].呼和浩特:内蒙古人民出版社,2000:299.
[4]国家中医药管理局《中华本草》编委会.中华本草.蒙药分卷[M].上海:上海科学技术出版社,2000:236.
[5]林俊芝,张定堃,裴瑾,等.基于植物代谢组学方法区分安县附子与江油附子质量的化学标志物[J].中国实验方剂学杂志,2017,23(13):35-40.
[6]刘秋月,黄文杰,王芬,等.甜菜碱药理活性及其机制研究进展[J].实用医药杂志,2016,33(4):371-374.
[7]徐倩,徐国兵.香豆素类化合物代谢研究进展[J].中国实验方剂学杂志,2015,21(3):222-225.
[8]ZHAO Z,Moghadasian M H.Chemistry,natural sources,dietary intake and pharmacokinetic properties of ferulic acid:a review[J].Food Chem,2008,109(4):691-702.
[9]Russo M,Spagnuolo,Tedesco I,et al.The flavonoid quercetin in disease prevention and therapy:facts and fancies[J].Biochem Pharmacol,2012,83(1):6-15.
[10]刘学仁,张莹,林志群.橙皮苷和橙皮素生物活性的研究进展[J].中国新药杂志,2011,20(4):329-333,381.
[11]黄佳颖,杨悦,李玉荣,等.芹菜素的研究进展[J].江苏科技信息,2015(4):57-58.
[12]Lee J H,Regmi S C,Kim J A,et al.Apple flavonoid phloretin inhibits Escherichia coli O157:H7 biofilm formation and ameliorates colon inflammation in rats[J].Infect Immun,2011,79(12):4819-4827.
[13]王继双,何焱,张文静,等.木犀草素的药理作用研究进展[J].生命科学,2013,25(6):560-565.
[14]童朝阳,徐琪寿.核黄素的药理作用及应用前景[J].军事医学科学院院刊,2003,27(3):223-226.
[15]张雅雯,邵东燕,师俊玲,等.山奈酚生物功能研究进展[J].生命科学,2017,29(4):400-405.
[16]Arshad N,Rashid N,Absar S,et al.UV-absorption studies of interaction of karanjin and karanjachromene with ds.DNA:evaluation of binding and antioxidant activity[J].Cent Eur J Chem,2013,11(12):2040-2047.
[17]杜佩玉.肝硬化合并肝源性糖尿病的护理体会[J].实用临床医药杂志,2014,18(24):170-171,173.
[18]杨娜,隋峰,姜廷良.神经性疾病相关的谷氨酸转运体研究进展[J].中国实验方剂学杂志,2011,17(7):255-258.
[19]高海霞,韩波,任华华.L-组氨酸催化合成乙酰水杨酸的研究[J].延安大学学报:自然科学版,2013,32(4):67-68.
[20]Hurrell R F,Reddy M B,Juillerat M,et al.Meat protein fractions enhance nonheme iron absorption in humans[J].J Nutr,2006,136(11):2808-2812.
[21]关静,霍艳艳,董春娇.烟酸类药物的研究进展[J].沈阳医学院学报,2011,13(2):110-112.
[22]杨驰,郑咏秋,戴敏.烟酰胺药理作用研究进展[J].临床肺科杂志,2011,16(12):1914-1916.
[23]余辉,申国庆.烟酰胺在皮肤科的应用进展[J].药学与临床研究,2014,22(3):262-265.
[24]马丛丛,许继取,韩领,等.芥子酸及其生物活性研究进展[J].中国油脂,2016,41(5):75-79.
[25]CHENG Z X,WU J J,LIU Z Q,et al.Development of a hydrophilic interaction chromatography-UPLC assay to determine trigonelline in rat plasma and its application in a pharmacokinetic study[J].Chin J Nat Med,2013,11(2):164-170.
[26]Rosso M D,Soligo S,Panighel A,et al.Changes in grape polyphenols(V.vinifera L.)as a consequence of postharvest withering by high-resolution mass spectrometry:Raboso Piave versus Corvina[J].J Mass Spectrom,2016,51(9):750-760.
[27]Aron P M,Kennedy J A.Flavan-3-ols:nature,occurrence and biological activity[J].Mol Nutr Food Res,2007,52(1):79-104.
[28]杨九凌,祝晓玲,李成文,等.咖啡酸及其衍生物咖啡酸苯乙酯药理作用研究进展[J].中国药学杂志,2013,48(8):577-581.
[29]鲁飞翔,胡南,周仙杰,等.硫胺素与相关疾病的研究进展[J].中华灾害救援医学,2016,4(5):287-290.
[30]安家慧,金玉姬,董传兴,等.精氨酸对机体的影响[J].吉林医药学院学报,2013,34(1):64-67.
[31]Tsai Y M,CHONG I W,Hung J Y,et al.Syringetin suppresses osteoclastogenesis mediated by osteoblasts in human lung adenocarcinoma[J].Oncol Rep,2015,34(2):617-626.
[32]代菊英.氨茶碱药理作用与临床应用[J].中国现代药物应用,2009,3(2):120-121.
[33]方莲花,吕扬,杜冠华.秦皮的药理作用研究进展[J].中国中药杂志,2008,33(23):2732-2736.
[34]韩向东.固沙先锋植物沙米的生物学特征研究[J].甘肃林业科技,2007,32(4):3-8.
[35]强永在,巴俊杰,渠弼,等.蒙药材沙蓬的生药学研究[J].时珍国医国药,2009,20(1):45-46.
[36]CHEN G,ZHAO J,ZHAO X,et al.A psammophyte Agriophyllum squarrosum(L.)Moq.:a potential food crop[J].Genet Resour Crop Ev,2014,61(3):669-676.
[37]任文明,刘雪峰,倪春梅.毛乌素沙漠天然沙米营养成分的分析[J].内蒙古农业大学学报:自然科学版,2005,26(2):88-90.
[38]QIAN C,YIN H,SHI Y,et al.Population dynamics of Agriophyllum squarrosum,a pioneer annual plant endemic to mobile sand dunes,in response to global climate change[J].Sci Rep,2016,doi:10.1038/srep26613.
[39]尹成亮,赵杰才,胡进玲,等.环境异质性对潜在粮食作物沙米表型变异的影响[J].中国科学:生命科学,2016,46(11):1324-1335.