青海地区汉族、藏族、回族乳腺癌患者中CXCL12和CXCR4的表达及意义
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摘要
目的探讨青海地区汉族、藏族、回族乳腺癌患者中趋化因子CXCL12及其受体CXCR4表达情况及其意义。方法收集青海大学附属医院乳腺甲状腺外科收治的汉族、藏族、回族各30例乳腺癌患者的组织标本,应用免疫组化SP法检测癌组织和癌旁正常组织中CXCL12和CXCR4表达情况,结合术后Ki-67、EGFR结果,分析CXCL12和CXCR4表达与患者Ki-67、EGFR、TNM分期、病理分级、淋巴结转移情况的关系。结果癌组织中CXCL12、CXCR4表达水平均明显高于癌旁正常乳腺组织(P均<0. 05),但汉、藏、回族乳腺癌患者癌组织中CXCL12、CXCR4表达阳性情况比较差异均无统计学意义(P均>0. 05); CXCL12、CXCR4表达与Ki-67、EGFR阳性表达、TNM分期和腋窝淋巴结转移有关(P均<0. 05),与病理学分级无关(P均> 0. 05)。结论青海地区汉族、藏族、回族乳腺癌患者间CXCL12、CXCR4表达情况无明显差异,而CXCL12、CXCR4表达情况与乳腺癌发展和转移密切相关,可作为合理预测乳腺癌浸润、转移等生物学行为的标记物。
Objective It is to explore the expression and significance of CXCL12 and CXCR4 in breast cancer patients of Han,Tibetan and Hui nationalities in Qinghai. Methods The tissue specimens of 30 patients of Han,Tibetan and Hui nationalities with breast cancer treated in department of thyroid breast surgery in the Affiliated Hospital of Qinghai University were collected. Immunohistochemical SP method was used to detect the expression of CXCL12 and CXCR4 in cancer tissues and adjacent normal tissues,the relationship between CXCL12 and CXCR4 expression and Ki-67,EGFR,TNM staging,pathological grade and lymph node metastasis were analyzed according to the Ki-67 and EGFR results. Results The expression levels of CXCL12 and CXCR4 in cancer tissues were significantly higher than those in adjacent normal breast tissues( P < 0. 05). However,there was no significant difference in the positive expressions of CXCL12 and CXCR4 between the cancer tissues of Han,Tibetan and Hui breast cancer patients( P > 0. 05); CXCL12 and CXCR4 expression were associated with Ki-67,EGFR positive expression,TNM stage and axillary lymph node metastasis( P < 0. 05),and were not related to pathological grade( P > 0. 05). Conclusion The expression of CXCL12 and CXCR4 were same among different nation,while CXCL12 and CXCR4 were closely related to the development and metastasis of breast cancer,which could be used as a marker to predict the biological behavior of breast cancer.
Objective It is to explore the expression and significance of CXCL12 and CXCR4 in breast cancer patients of Han,Tibetan and Hui nationalities in Qinghai. Methods The tissue specimens of 30 patients of Han,Tibetan and Hui nationalities with breast cancer treated in department of thyroid breast surgery in the Affiliated Hospital of Qinghai University were collected. Immunohistochemical SP method was used to detect the expression of CXCL12 and CXCR4 in cancer tissues and adjacent normal tissues,the relationship between CXCL12 and CXCR4 expression and Ki-67,EGFR,TNM staging,pathological grade and lymph node metastasis were analyzed according to the Ki-67 and EGFR results. Results The expression levels of CXCL12 and CXCR4 in cancer tissues were significantly higher than those in adjacent normal breast tissues( P < 0. 05). However,there was no significant difference in the positive expressions of CXCL12 and CXCR4 between the cancer tissues of Han,Tibetan and Hui breast cancer patients( P > 0. 05); CXCL12 and CXCR4 expression were associated with Ki-67,EGFR positive expression,TNM stage and axillary lymph node metastasis( P < 0. 05),and were not related to pathological grade( P > 0. 05). Conclusion The expression of CXCL12 and CXCR4 were same among different nation,while CXCL12 and CXCR4 were closely related to the development and metastasis of breast cancer,which could be used as a marker to predict the biological behavior of breast cancer.
引文
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[15]Rahimi M,Tang CK.CXCR4 Suppression Attenuates EG-FRv III-Mediated Invasion and Induces p38 MAPK-Dependent Protein Trafficking and Degradation of EGFRv IIIin Breast Cancer Cells[J].Cancer Lett,2011,306(1):43-51
[16]马会清,杨秀凤,程海燕.宫颈鳞癌中基质细胞衍生因子-1、CXC趋化因子受体4、基质金属蛋白酶-2和Ki-67的表达及意义[J/CD].中华妇幼临床医学杂志:电子版,2014,10(2):74-78
[2]Park JM,Munoz JL,Won BW,et al.Exogenous CXCL12activates protein kinase C to phosphorylate connexin 43for gap junctional intercellular communication among confluent breast cancer cells[J].Cancer Lett,2013,331(1):84-91
[3]Soon PS,Kim E,Pon CK,et al.Breast cancer-associated fibroblasts induce epithelial-to-mesenchymal transition in breast cancer cells[J].Endocr Relat Cancer,2013,20(1):1-12
[4]Iwasa S,Yanagawa T,Fan J,et al.Expression of CXCR4and its ligand SDF-1 in intestinal-type gastric cancer is associated with lymph node and liver metastasis[J].Anticancer Res,2009,29(11):4751-4758
[5]Hinton CV,Avraham S,Avraham HK.Role of the CX-CR4/CXCL12 signaling axis in breast cancer metastasis to the brain[J].Clin Exp Metastasis,2010,27(2):97-105
[6]Balkwill F.Cancer and the chemokine network[J].Nat Rev Cancer,2004,4(7):540-550
[7]Muller A,Homey B,Soto H,et al.Involvement of chemokine receptors in breast cancer metastasis[J].Nature,2001,410(6824):50-56
[8]Choi WT,Yang Y,Xu Y,et al.Targeting Chemokine Receptor CXCR4 for Treatment of HIV-1 Infection,Tumor Progression,and Metastasis[J].Curr Top Med Chem,2014,14(13):1574-1589
[9]Allinen M,Beroukhim R,Cai L,et al.Molecular characterization of the tumor microenvironment in breast cancer[J].Cancer Cells,2008,6(1):17-32
[10]Barbero S,Bonavia R,Bajetto A,et al.Stromal cell-derived factor 1 alpha stimulates human glioblastoma cell growth through the activation of both extracellular signalregulated kinases 1/2 and Akt[J].Cancer Res,2003,63(8):1969-1974
[11]Schmid BC,Rudas M,Rezniczek GA,et al.CXCR4 is expressed in ductal carcinona in situ of the breast and inatypical ductal hyperplasia[J].Breast Cancer Res Treat,2004,84(3):247-250
[12]Sun Y,Mao X,Fan C,et al.CXCL12-CXCR4 axis promotes the natural selection of breast cancer cell metastasis[J].Tumor Biol,2014,35(8):7765-7773
[13]Liu Y,Ji R,Li J,et al.Correlation effect of EGFR and CXCR4 and CCR7 chemokine receptors in predicting breast cancer metastasis and prognosis[J].J Exp Clin Cancer Res,2010,29(1):16-33
[14]Li R,Huang W,Wu J,et al.EGFR expression is associated with cytoplasmic staining of CXCR4 and predicts poor prognosis in triple-negative breast carcinomas[J].Oncol Lett,2017,13(2):695-703
[15]Rahimi M,Tang CK.CXCR4 Suppression Attenuates EG-FRv III-Mediated Invasion and Induces p38 MAPK-Dependent Protein Trafficking and Degradation of EGFRv IIIin Breast Cancer Cells[J].Cancer Lett,2011,306(1):43-51
[16]马会清,杨秀凤,程海燕.宫颈鳞癌中基质细胞衍生因子-1、CXC趋化因子受体4、基质金属蛋白酶-2和Ki-67的表达及意义[J/CD].中华妇幼临床医学杂志:电子版,2014,10(2):74-78