HPLC-ELSD法测定银杏内酯B脂微球中溶血磷脂酰胆碱的含量
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摘要
目的:建立HPLC-ELSD法测定银杏内酯B脂微球中溶血磷脂酰胆碱的含量。方法:采用硅胶色谱柱(Platisil Silica,250mm×4.6mm,5μm),流动相为甲醇-冰醋酸(500∶10,三乙胺调节pH值至6.0),流速为1.0mL·min~(-1),柱温为40℃,检测器为蒸发光散射检测器。结果:溶血磷脂酰胆碱在38.4～480μg·mL~(-1)时,线性关系良好(r=0.9987),平均回收率(n=9)为102.13%,RSD为3.46%。结论:该方法准确,重现性好,可用于银杏内酯B脂微球中溶血磷脂酰胆碱的含量测定。
Objective:To establish an HPLC method for determination of lyso-phosphatidycholine in Ginkgolide B lipid microsphere. Methods:Chromatographic column was silicone column(Platisil Silica, 250 mm×4.6 mm,5μm),mobile phase was methanol-glacial acetic acid(500∶10, adjusting pH to 6.0 with triethylamine), the flow rate was1.0 mL·min~(-1), the column temperature was set at 40℃, and the detector was ELSD. Results:Lyso-phosphatidycholinehad a good linear relationin the rang of 38.4 ～480μg·mL~(-1)(r=0.9987).The average recovery was 102.13%with the RSD of 3.46%(n=9). Conclusion:The method is accurate, reproducible and applicable for determination of lyso-phosphatidycholine in Ginkgolide B lipid microsphere.
Objective:To establish an HPLC method for determination of lyso-phosphatidycholine in Ginkgolide B lipid microsphere. Methods:Chromatographic column was silicone column(Platisil Silica, 250 mm×4.6 mm,5μm),mobile phase was methanol-glacial acetic acid(500∶10, adjusting pH to 6.0 with triethylamine), the flow rate was1.0 mL·min~(-1), the column temperature was set at 40℃, and the detector was ELSD. Results:Lyso-phosphatidycholinehad a good linear relationin the rang of 38.4 ～480μg·mL~(-1)(r=0.9987).The average recovery was 102.13%with the RSD of 3.46%(n=9). Conclusion:The method is accurate, reproducible and applicable for determination of lyso-phosphatidycholine in Ginkgolide B lipid microsphere.
引文
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[9]晏敏红,陈应林,钟己未.HPLC-ELSD测定尼莫地平脂肪乳注射液中溶血磷脂酰胆碱的含量[J].中国药师,2014,17(1):87-90.
[2]张学非,曹泽彧,许治良,等.银杏内酯治疗脑缺血作用机制的研究进展[J].中草药,2016,47(16):2943-2948.
[3]王旋,张慧灵,顾振纶,等.银杏内酯药理作用的研究进展[J].中草药,2005,36(11):1741-1744.
[4]Liu ZZ,Feng YY,Zhang LL,et al.Pharmacokinetics and tissue distribution of larotaxel in rats:comparison of larotaxel-loaded microsphere with larotaxel-solution[J].Cancer Chemother Pharmacol,2013,71(5):1131-1139.
[5]江桂生,苏健芬,付寒,等.HPLC-ELSD测定肠外营养用海豹油脂肪乳中溶血磷脂酰胆碱的含量[J].中国现代应用药学,2012,29(5):440-444.
[6]王全一,矫筱蔓.HPLC-ELSD法测定前列地尔注射液中溶血磷脂酰胆碱的方法改进[J].药物分析杂志,2014,34(7):1291-1295.
[7]傅军霞,姚芳,胡爱珍,等.前列地尔冻干脂微球的制备及其体内外评价[J].中国药学杂志,2015,50(19):1704-1708.
[8]陈晓瑾,严俊.甘草酸二铵磷脂复合物注射剂中溶血磷脂酰胆碱的稳定性研究[J].药物分析杂志,Chin J Pharm Anal,2014,34(11):2044-2047.
[9]晏敏红,陈应林,钟己未.HPLC-ELSD测定尼莫地平脂肪乳注射液中溶血磷脂酰胆碱的含量[J].中国药师,2014,17(1):87-90.