模拟牵张成骨多单元细胞拉伸装置的研制与应用评价
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摘要
目的本实验拟研制一种细胞加力装置用以模拟成骨细胞在牵张成骨过程中所受的力学刺激。方法设计研制多单元细胞拉伸装置,检测该装置的机械性能和细胞培养性能,用硅胶膜长轴形变量1%、5%、10%、15%的单轴静态牵张力进行测试,选择成骨细胞在该装置所受的最佳单轴静态牵张力刺激。结果本实验所用的硅胶膜对成骨细胞没有细胞毒性。机械性能和细胞培养实验结果表明本实验研制的多单元细胞拉伸装置机械性能精确可靠,具有良好的密闭性,操作观察方便。细胞增殖实验表明10%单轴静态牵张力对成骨细胞的促增殖能力最强。结论多单元细胞拉伸装置对成骨细胞施加的10%单轴静态牵张力能模拟在长骨、颅颌面骨牵张成骨过程中成骨细胞受到的持续性拉伸力,为进一步研究牵张成骨的分子机制提供实验基础。
Objective To develop a multiple-unit uniaxial static stretching device to simulate the uniaxial mechanical environment of osteoblasts in distraction osteogenesis( DO). Methods A multiple-unit uniaxial static stretching device was developed and its mechanical properties and cell culture performance were validated. 1%,5%,10% and 15% uniaxial static stretches were tested to obtain appropriate mechanical stimulation on osteoblasts. Results Silica membrane has no cytotoxicity to osteoblasts. The multiple-unit uniaxial static stretching device was an effective and reliable system and 10% uniaxial static stretch was optimal to promote proliferation of osteoblasts. Conclusion 10% uniaxial static stretch on osteoblasts from the multiple-unit uniaxial static stretching device can simulate the mechanical environment in vivo in DO of long bone and craniomaxillofacial bone,which provides the experimental basis to investigate the molecular mechanisms underlying DO further.
Objective To develop a multiple-unit uniaxial static stretching device to simulate the uniaxial mechanical environment of osteoblasts in distraction osteogenesis( DO). Methods A multiple-unit uniaxial static stretching device was developed and its mechanical properties and cell culture performance were validated. 1%,5%,10% and 15% uniaxial static stretches were tested to obtain appropriate mechanical stimulation on osteoblasts. Results Silica membrane has no cytotoxicity to osteoblasts. The multiple-unit uniaxial static stretching device was an effective and reliable system and 10% uniaxial static stretch was optimal to promote proliferation of osteoblasts. Conclusion 10% uniaxial static stretch on osteoblasts from the multiple-unit uniaxial static stretching device can simulate the mechanical environment in vivo in DO of long bone and craniomaxillofacial bone,which provides the experimental basis to investigate the molecular mechanisms underlying DO further.
引文
[1]胡静,王大章.颌面骨骼整形手术图谱[M].北京:人民卫生出版社,2013:160-161.
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[9]Toworfe GK,Composto RJ,Lee MH,et al.Elastic membrane that undergoes mechanical deformation enhances osteoblast cellular attachment and proliferation[J].Int J Biomater,2010,1(2):1-10.
[10]Kaspar D,Seidl W,Neidlinger-Wilke C,et al.In vitro effects of dynamic strain on the proliferative and metabolic activity of human osteoblasts[J].J Musculoskelet Neuronal Interact,2000,1(2):161-164.
[11]Bhatt KA,Chang EI,Warren SM,et al.Uniaxial mechanical strain:an in vitro correlate to distraction osteogenesis[J].J Surg Res,2007,143(2):329-336.
[12]Neidlinger-Wilke C,Wilke HJ,Claes L.Cyclic stretching of human osteoblasts affects proliferation and metabolism:a new experimental method and its application[J].J Orthop Res,1994,12(1):70-78.
[2]王兴,王晓霞.颅颌面部骨骼牵引成骨[M].北京:人民卫生出版社,2011:703.
[3]李晓峰,赵劲民,苏伟,等.大鼠成骨细胞的原代培养和鉴定[J].中国组织工程研究与临床康复,2011,15(6):990-994.
[4]Fong KD,Nacamuli RP,Loboa EG,et al.Equibiaxial tensile strain affects calvarial osteoblast biology[J].J Craniofac Surg,2003,14(3):348-355.
[5]Ignatius A,Blessing H,Liedert A,et al.Tissue engineering of bone:effects of mechanical strain on osteoblastic cells in type I collagen matrices[J].Biomaterials,2005,26(3):311-318.
[6]邓红文,刘耀中.骨生物学前沿[M].北京:高等教育出版社,2006:233.
[7]Wang H,Kwok DT,Wang W,et al.Osteoblast behavior on polytetrafluoroethylene modified by long pulse,high frequency oxygen plasma immersion ion implantation[J].Biomaterials,2010,31(3):413-419.
[8]Winter LC,Walboomers XF,Bumgardner JD,et al.Intermittent versus continuous stretching effects on osteoblast-like cells in vitro[J].J Biomed Mater Res A,2003,67(4):1269-1275.
[9]Toworfe GK,Composto RJ,Lee MH,et al.Elastic membrane that undergoes mechanical deformation enhances osteoblast cellular attachment and proliferation[J].Int J Biomater,2010,1(2):1-10.
[10]Kaspar D,Seidl W,Neidlinger-Wilke C,et al.In vitro effects of dynamic strain on the proliferative and metabolic activity of human osteoblasts[J].J Musculoskelet Neuronal Interact,2000,1(2):161-164.
[11]Bhatt KA,Chang EI,Warren SM,et al.Uniaxial mechanical strain:an in vitro correlate to distraction osteogenesis[J].J Surg Res,2007,143(2):329-336.
[12]Neidlinger-Wilke C,Wilke HJ,Claes L.Cyclic stretching of human osteoblasts affects proliferation and metabolism:a new experimental method and its application[J].J Orthop Res,1994,12(1):70-78.