小麦籽粒清蛋白和球蛋白条件和非条件QTL分析
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摘要
小麦蛋白含量的提高对营养和加工品质具有重要的意义。本研究在2年3点6个环境下,以小麦品种"花培3号×豫麦57"构建的双单倍体(doubled haploid,DH)群体为材料,通过条件和非条件QTL鉴定与小麦开花后5个时期籽粒清蛋白和球蛋白含量积累有关的基因或数量性状基因座。结果表明,基于完备区间作图法,共检测到40个非条件QTLs和34个条件QTLs,单个位点的贡献率范围为6.44%~25.13%,其中QAlu1B-3在6个环境下的第4和第5时期均被检测出。本研究结果为了解不同灌浆期QTL选择性表达奠定了理论基础,检测到的QTL可能对小麦籽粒品质的提高有重要贡献。
The improvement of wheat protein-fraction contents is important in wheat breeding for nutritional and processing quality.A set of 168 doubled-haploid lines was used to identify dynamic expression of genes or quantitative trait loci(QTLs)associated with accumulation of different proteinfractions through conditional and unconditional analysis of five post-flowering stages of wheat at three locations over 2 years.The QTLs were analyzed using an inclusive composite interval mapping approach.A total of 40 unconditional QTLs and 34 conditional QTLs for protein-fraction contents were detected and individual QTLs in the six environments explained between 6.44% and 25.13% of observed phenotypic variation.,among which QAlu1 B-3 was persistently expressed at 2 stages in six environments.The results provide a theoretical base for understanding the genetic basis of selective expression of QTLs during different grain-filling stages.The detected QTLs may have important contributions to grain quality in wheat.
The improvement of wheat protein-fraction contents is important in wheat breeding for nutritional and processing quality.A set of 168 doubled-haploid lines was used to identify dynamic expression of genes or quantitative trait loci(QTLs)associated with accumulation of different proteinfractions through conditional and unconditional analysis of five post-flowering stages of wheat at three locations over 2 years.The QTLs were analyzed using an inclusive composite interval mapping approach.A total of 40 unconditional QTLs and 34 conditional QTLs for protein-fraction contents were detected and individual QTLs in the six environments explained between 6.44% and 25.13% of observed phenotypic variation.,among which QAlu1 B-3 was persistently expressed at 2 stages in six environments.The results provide a theoretical base for understanding the genetic basis of selective expression of QTLs during different grain-filling stages.The detected QTLs may have important contributions to grain quality in wheat.
引文
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[17]MCINTOSH R A.Catalogue of gene symbols for wheat[J].Cereal Research Communications,1975,3(1):69.
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[19]刘丽,李卫华,刘伟,等.小麦谷蛋白膨胀指数发育动态的QTL分析[J].中国农业科学,2008,41(11):3838.LIU L,LI WH,LIU W,et al.QTL analysis of developmental stages of wheat glutenin expansion index[J].Scientia Agricultura Sinica,2008,41(11):3838.
[20]朱占玲,刘宾,田宾,等.小麦籽粒蛋白质含量的动态QTL分析[J].中国农业科学,2011,44(15):3078.ZHU Z L,LIU B,TIAN B,et al.Dynamic QTL analysis of wheat grain protein content[J].Scientia Agricultura Sinica,2011,44(15):3078.
[21]ZHAO L,ZHANG KP,LIU B,et al.A comparison of grain protein content QTLs and flour protein content QTLs across environments in cultivated wheat[J].Euphytica,2010,174(3):325.
[22]TANKSLEY S D.Mapping polygenes[J].Annual Review of Genetics,1993,27:205.
[23]李泽福,周彤,郑天清,等.水稻抽穗期QTL与环境互作分析[J].作物学报,2002,28(6):771.LI Z F,ZHOU T,ZHEN T Q,et al.Analysis of interaction between QTL and environment at heading stage in rice[J].The Crop Journal,2002,28(6):771.
[2]MARIELLE M,PHILIPPE L,CHRISTOPHE C,et al.Mapping and proteomic analysis of albumin and globulin proteins in hexaploid wheat kernels[J].Theoretical and Applied Genetics,2009,118(7):1321.
[3]DUPONT F M,ALTENBACH S B.Molecular and biochemical impacts of environmental factors on wheat grain development and protein synthesis[J].Journal of Cereal Science,2003,38(2):133.
[4]杨学举,张树华,荣广哲.小麦蛋白质组分含量的配合力和遗传力分析[J].遗传,1999,21(4):34.YANG X J,ZAHNG S H,RONG G Z.Combining ability and heritability of protein content in wheat[J].Hereditas,1999,21(4):34.
[5]吴为人,李维明,卢浩然.数量性状基因座的动态定位策略[J].生物数学学报,1997,12(5):490.WU W R,LI W M,LU H R.Dynamic locating strategy of quantitative trait loci[J].Journal of Biomathematics,1997,12(5):490.
[6]ZHANG L Y,LI H H,LI Z L,et al.Interactions between markers can be caused by the dominance effect of quantitative trait loci[J].Genetics,2008,180:1177.
[7]刘宾,赵亮,张坤普,等.小麦株高发育动态QTL定位[J].中国农业科学,2010,43(22):4562.LIU B,ZHAO L,ZHANG K,et al.QTL mapping of wheat plant height development[J].Scientia Agricultura Sinica,2010,43(22):4562.
[8]WU X S,WANG Z H,CHANG X P,et al.Genetic dissection of the developmental behaviours of plant height in wheat under diverse water regimes[J].Journal of Experiment Botany,2010,61(11):2923.
[9]WANG Z H,WU X S,REN Q,et al.QTL mapping for developmental behavior of plant height in wheat(Triticum aestivum L.)[J].Euphytica,2010,174:447.
[10]石培春,曹连莆,王光利,等.小麦籽粒蛋白质组分含量的QTL定位[J].麦类作物学报,2008,28(4):550.SHI P C,CAO L P,WANG G L,et al.QTL mapping of protein content in wheat grain[J].Journnal of Triticeae Crops,2008,28(4):550.
[11]孙海艳,吕建华,范玉顶,等.小麦蛋白质和淀粉品质性状的QTL分析[J].自然科学进展,2008,18(5):505.SU H Y,LJ H,FAN Y D,et al.QTL analysis of protein and starch quality traits in wheat[J].Progress in Natural Science,2008,18(5):505.
[12]ZHANG Y,TANG J W,ZHANG Y L,et al.QTL mapping for quantities of protein fractions in bread wheat(Triticum aestivum L.)[J].Theoretical and Applied Genetics,2011,122(5):971.
[13]LI J,CUI F,DING A,et al.QTL detection of seven quality traits in wheat using two related recombinant inbred line populations[J].Euphytica,2012,183(2):207.
[14]海燕,康明辉.高产早熟小麦新品种花培3号的选育[J].河南农业科学,2007(5):36.HAN Y,KANG M H.Breeding of a new high-yielding and early-maturing wheat new variety Huapei 3[J].Journal of Nenan Agricultural Sciences,2007(5):36-37.
[15]郭春强,柏志安,廖平安,等.优质高产小麦新品种豫麦57[J].中国种业,2004(4):54.GUO C Q,BAI Z A,LIAO P A,et al.Quality and high yield of wheat variety Yumai 57[J].China Seed Industry,2004(4):54.
[16]何照范.粮油籽粒品质及其分析技术[M].北京:中国农业出版社,1985:290.HE Z F.Grain and oil grain quality and its analytical techniques[M].Beijing:China Agriculture Press,1985:290.
[17]MCINTOSH R A.Catalogue of gene symbols for wheat[J].Cereal Research Communications,1975,3(1):69.
[18]张国平.国外谷物种子蛋白的研究现状[J].世界农业,1987(12):23.ZHANG G P.Research status of foreign grain seed protein[J].World Agriculture,1987(12):23.
[19]刘丽,李卫华,刘伟,等.小麦谷蛋白膨胀指数发育动态的QTL分析[J].中国农业科学,2008,41(11):3838.LIU L,LI WH,LIU W,et al.QTL analysis of developmental stages of wheat glutenin expansion index[J].Scientia Agricultura Sinica,2008,41(11):3838.
[20]朱占玲,刘宾,田宾,等.小麦籽粒蛋白质含量的动态QTL分析[J].中国农业科学,2011,44(15):3078.ZHU Z L,LIU B,TIAN B,et al.Dynamic QTL analysis of wheat grain protein content[J].Scientia Agricultura Sinica,2011,44(15):3078.
[21]ZHAO L,ZHANG KP,LIU B,et al.A comparison of grain protein content QTLs and flour protein content QTLs across environments in cultivated wheat[J].Euphytica,2010,174(3):325.
[22]TANKSLEY S D.Mapping polygenes[J].Annual Review of Genetics,1993,27:205.
[23]李泽福,周彤,郑天清,等.水稻抽穗期QTL与环境互作分析[J].作物学报,2002,28(6):771.LI Z F,ZHOU T,ZHEN T Q,et al.Analysis of interaction between QTL and environment at heading stage in rice[J].The Crop Journal,2002,28(6):771.