摘要
为了探索环介导等温扩增技术(LAMP)在牦牛粪便阿米巴原虫检测中的实用性,利用阿米巴原虫的18S rRNA基因序列进行引物设计,在优化LAMP检测方法的同时,对该方法在进行阿米巴原虫检测时的特异性、敏感性、重复性进行分析。结果显示:已建立的阿米巴原虫LAMP检测方法具有一定的特异性,敏感性优于PCR,并具有良好的重复性;在临床应用性方面,其检出率与利用传统PCR得到的检出率完全相同。结果表明:该LAMP检测方法可用于牦牛粪便中阿米巴原虫的检测。
In order to explore the applicability of loop-mediated isothermal amplification(LAMP) in detection of Entamoeba and to improve the detection efficiency of Entamoeba in Yak feces, primers were designed using the 18S rRNA gene of Entamoeba to establish the detection method of LAMP and to analyze the specificity, sensitivity, repeatability and clinical application of the method. The results showed the LAMP method established here only had a certain specificity, better sensitivity and repeatability, but also had clinical applicability, because the positive rate of the yak feces samples detected by LAMP was the same as that detected by traditional PCR. Therefore, the LAMP method can be applied to the detection of Entamoeba in feces of yak.
引文
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