甘露寡糖对泥鳅生产性能、肠道菌群和非特异性免疫功能的影响
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摘要
【目的】探讨甘露寡糖对泥鳅生产性能、肠道菌群和非特异性免疫功能的影响。【方法】选择健康均重为(3.27±0.05)g泥鳅360尾,随机分为6组,每组3个重复,每个重复20尾。在基础饲料中分别添加0(对照组)、100、200、400、600和800 mg/kg甘露寡糖。试验期70 d。检测成活率、特定生长率、肥满度、饵料系数、肠体指数、肠长指数,肠道大肠埃希菌、乳酸杆菌、双歧杆菌以及血浆和肝胰脏溶菌酶、超氧化物歧化酶、过氧化氢酶等指标。【结果】①200和400 mg/kg组特定生长率、肥满度、肠体指数和肠长指数显著高于对照组,饵料系数显著低于对照组(P<0.05);②200和400 mg/kg组肠道乳酸杆菌和双歧杆菌数量显著高于对照组,大肠埃希菌数量显著低于对照组(P<0.05);③200和400 mg/kg组血浆和肝胰脏SOD、CAT活性显著高于对照组(P<0.05),血浆LZM含量显著高于对照组(P<0.05)。【结论】在饲料中添加200～400 mg/kg甘露寡糖可促进泥鳅肠道发育,优化肠道微生态环境,显著提高其非特异性免疫功能和生长性能。
【Objective】The experiment was conducted to study the effects of mannose oligosaccharide on productive performance,intestinal flora and non-specific immune function of Misgurnus anguillicaudatus.【Method】360 healthy M. anguillicaudatus with an average body weight of(3.27 ± 0.05) g were selected,and randomly divided into 6 groups with 3 replicates and 20 individuals per replicate. 0(control group),100,200,400,600 and 800 mg/kg of mannose oligosaccharide were added to the basal diet,respectively. Various indexes of production performance,intestinal flora and non-specific immune function were detected after 70-day breeding.【Result】① The specific growth rate,condition factor,intestinal weight indexes and intestinal length indexes in 200 mg/kg and 400 mg/kg groups were significantly higher while feed coefficient s were significantly lower than those in control group(P<0.05); ②The amount of Lactobacillus and Bifidobacterium in 200 mg/kg and 400 mg/kg groups were significantly higher but the amount of Escherichia coil were significantly lower than those of control group(P<0.05);③Compared with control group,SOD and CAT activities in plasma,hepatopancreas and LZM in plasma of 200 mg/kg and 400 mg/kg groups were significantly increased(P<0.05).【Conclusion】Diet with 200-400 mg/kg mannose oligosaccharide can significantly promote intestinal development,optimize micro-ecology circumstances,and improve non-specific immunity function and growth performance of M. anguillicaudatus.
【Objective】The experiment was conducted to study the effects of mannose oligosaccharide on productive performance,intestinal flora and non-specific immune function of Misgurnus anguillicaudatus.【Method】360 healthy M. anguillicaudatus with an average body weight of(3.27 ± 0.05) g were selected,and randomly divided into 6 groups with 3 replicates and 20 individuals per replicate. 0(control group),100,200,400,600 and 800 mg/kg of mannose oligosaccharide were added to the basal diet,respectively. Various indexes of production performance,intestinal flora and non-specific immune function were detected after 70-day breeding.【Result】① The specific growth rate,condition factor,intestinal weight indexes and intestinal length indexes in 200 mg/kg and 400 mg/kg groups were significantly higher while feed coefficient s were significantly lower than those in control group(P<0.05); ②The amount of Lactobacillus and Bifidobacterium in 200 mg/kg and 400 mg/kg groups were significantly higher but the amount of Escherichia coil were significantly lower than those of control group(P<0.05);③Compared with control group,SOD and CAT activities in plasma,hepatopancreas and LZM in plasma of 200 mg/kg and 400 mg/kg groups were significantly increased(P<0.05).【Conclusion】Diet with 200-400 mg/kg mannose oligosaccharide can significantly promote intestinal development,optimize micro-ecology circumstances,and improve non-specific immunity function and growth performance of M. anguillicaudatus.
引文
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[3]张红梅,姜会民.甘露寡糖对鲤鱼生产性能的影响[J].饲料研究,2004(9):33-34.
[4]马志红,晋晶,刘红梅,等.甘露寡糖对鲤鱼生长性能和免疫功能的影响[J].安徽农业科学,2010, 38(3):1291-1292, 1296.
[5]李洋.甘露寡糖对幼建鲤生长性能和非特异性免疫的影响[D].雅安:四川农业大学,2011.
[6] HELLAND B G, STALE J, DELBERT M. The effects of dietary supplementation with mannan oligosaeeharide, fruetooligosaccharide or galaetooligosaeeharide on the growth and feed utilization of Atlantic salmon(Salmo salar)[J].Aquaeulture, 2008(283):163-167.
[7]刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼(Oreochramis niloticus×O.aureus)生长、肠道结构和非特异性免疫的影响[J].浙江大学学报(农业与生命科学版),2009, 35(3):329-336.
[8]马相杰,汪立平,赵勇,等.甘露寡糖对罗非鱼幼鱼肠道微生物的影响[J].微生物学通报,2010, 37(5):708-713.
[9] DIMITROGLOU A, MERRIFIELD D L, SPRING P, et al. Effeets of mannan oligosaeeharide(MOS)supplementation on growth performanee,feed utilisation, intestinal histology and gut mierobiota of gilthead sea bream(Sparus aurata)[J]. Aquaeulture,2010(300):182-188.
[10]于艳梅,吴志新,陈孝煊,等.魔芋甘露寡糖对黄颡鱼非特异性免疫功能及生长的影响[J].华中农业大学学报,2010, 29(3):351-355.
[11]黄辨非,徐海.甘露寡糖对斑点叉尾鮰成活率及生长的影响[J].渔业现代化,2010, 37(3):34-36, 47.
[12] SILVIA T, ALEX M. Reduced gut bacterial translocation in European sea bass(Dicentrarchus lab rax)fed mannan oligoccharides(MOS)[J]. Fish&Shellfish Immunology, 2011(30):674-681.
[13]陈希,吴志新,刘佳佳,等.甘露寡糖对草鱼血清生化特性的影响[J].南昌大学学报(理科版),2011,35(4):393-397, 402.
[14] PRYOR G S, ROYES J B, CHAPMAN F A, et al. Mannan oligosaeeharide in fish nutrition:effeets of dietary Supplementation on growth and gastrointestinal villi strueture in gulf of Mexieo sturgeon[J]. North Ameriean journal of aquaeulture, 2003(65):106-111.
[15]李富东.果寡糖、甘露寡糖和芽孢杆菌对牙虾免疫功能和肠道微生态的影响[D].厦门:集美大学,2010.
[16]徐磊,刘波,谢骏,等.甘露寡糖对异育银鲫生长性能、免疫及HSP70基因表达的影响[J].水生生物学报,2012, 36(4):656-664.
[17]杜宗君,夏晶,温安祥.谷氨酰胺对嗜水气单胞菌致病中华鳖的保护作用[J].四川动物,2014, 33(2):254-260.
[18] TOORRECILLAS S, MAKOI A, CABALLERO M J, et al. Immune stimulation and improved infection resist ance in European sea bass(Dicentrarchus labrax)fed mannan oligosacchafdes[J].Fish&Shellfish Immunology,2007,23:969-981.
[2]李云兰.甘露寡糖对幼建鲤(Gyprinus carpio Var. Jian)肠道菌群和免疫功能的影响[D].雅安:四川农业大学,2004.
[3]张红梅,姜会民.甘露寡糖对鲤鱼生产性能的影响[J].饲料研究,2004(9):33-34.
[4]马志红,晋晶,刘红梅,等.甘露寡糖对鲤鱼生长性能和免疫功能的影响[J].安徽农业科学,2010, 38(3):1291-1292, 1296.
[5]李洋.甘露寡糖对幼建鲤生长性能和非特异性免疫的影响[D].雅安:四川农业大学,2011.
[6] HELLAND B G, STALE J, DELBERT M. The effects of dietary supplementation with mannan oligosaeeharide, fruetooligosaccharide or galaetooligosaeeharide on the growth and feed utilization of Atlantic salmon(Salmo salar)[J].Aquaeulture, 2008(283):163-167.
[7]刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼(Oreochramis niloticus×O.aureus)生长、肠道结构和非特异性免疫的影响[J].浙江大学学报(农业与生命科学版),2009, 35(3):329-336.
[8]马相杰,汪立平,赵勇,等.甘露寡糖对罗非鱼幼鱼肠道微生物的影响[J].微生物学通报,2010, 37(5):708-713.
[9] DIMITROGLOU A, MERRIFIELD D L, SPRING P, et al. Effeets of mannan oligosaeeharide(MOS)supplementation on growth performanee,feed utilisation, intestinal histology and gut mierobiota of gilthead sea bream(Sparus aurata)[J]. Aquaeulture,2010(300):182-188.
[10]于艳梅,吴志新,陈孝煊,等.魔芋甘露寡糖对黄颡鱼非特异性免疫功能及生长的影响[J].华中农业大学学报,2010, 29(3):351-355.
[11]黄辨非,徐海.甘露寡糖对斑点叉尾鮰成活率及生长的影响[J].渔业现代化,2010, 37(3):34-36, 47.
[12] SILVIA T, ALEX M. Reduced gut bacterial translocation in European sea bass(Dicentrarchus lab rax)fed mannan oligoccharides(MOS)[J]. Fish&Shellfish Immunology, 2011(30):674-681.
[13]陈希,吴志新,刘佳佳,等.甘露寡糖对草鱼血清生化特性的影响[J].南昌大学学报(理科版),2011,35(4):393-397, 402.
[14] PRYOR G S, ROYES J B, CHAPMAN F A, et al. Mannan oligosaeeharide in fish nutrition:effeets of dietary Supplementation on growth and gastrointestinal villi strueture in gulf of Mexieo sturgeon[J]. North Ameriean journal of aquaeulture, 2003(65):106-111.
[15]李富东.果寡糖、甘露寡糖和芽孢杆菌对牙虾免疫功能和肠道微生态的影响[D].厦门:集美大学,2010.
[16]徐磊,刘波,谢骏,等.甘露寡糖对异育银鲫生长性能、免疫及HSP70基因表达的影响[J].水生生物学报,2012, 36(4):656-664.
[17]杜宗君,夏晶,温安祥.谷氨酰胺对嗜水气单胞菌致病中华鳖的保护作用[J].四川动物,2014, 33(2):254-260.
[18] TOORRECILLAS S, MAKOI A, CABALLERO M J, et al. Immune stimulation and improved infection resist ance in European sea bass(Dicentrarchus labrax)fed mannan oligosacchafdes[J].Fish&Shellfish Immunology,2007,23:969-981.