高效液相色谱法测定鲁米诺试剂稳定性研究
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摘要
建立了鲁米诺试剂体系的高效液相色谱分析方法,并用该方法测试了鲁米诺体系中鲁米诺的稳定性。结果表明,优化实验条件下,鲁米诺的保留时间为4. 282 min,出峰效果较好,标准溶液在0. 1～4 mmol/L范围内呈现良好的线性关系,平均加标回收率为90. 31%～99. 05%,相对标准偏差RSD≤2. 93%。在10周内鲁米诺试剂没有明显变化,色谱峰面积偏差≤5%,稳定性良好,该方法具有操作简便、结果准确、精密度良好的优点,可用于对鲁米诺试剂进行检测分析。
HPLC Method for the determination of luminol reagent system has been established,and the stability of luminol has been tested.The results showed that the retention time of luminol was 4. 282 min.Strong linear relationship was observed from the calibration curve of luminol in the concentration of 0. 1 ～ 4 mmol/L,the average recovery was 90. 31% ～ 99. 05% with RSD ≤2. 93%.No significant change was observed within 10 weeks for luminol reagent.The chromatographic peak was stable,and peak area deviation of the luminol reagent was ≤5%.The established chromotography is convenient,accurate and suitable for application of detection and analysis of luminol reagent.
HPLC Method for the determination of luminol reagent system has been established,and the stability of luminol has been tested.The results showed that the retention time of luminol was 4. 282 min.Strong linear relationship was observed from the calibration curve of luminol in the concentration of 0. 1 ～ 4 mmol/L,the average recovery was 90. 31% ～ 99. 05% with RSD ≤2. 93%.No significant change was observed within 10 weeks for luminol reagent.The chromatographic peak was stable,and peak area deviation of the luminol reagent was ≤5%.The established chromotography is convenient,accurate and suitable for application of detection and analysis of luminol reagent.
引文
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[14]张可冬,李慧勇,谭建华,等.高效液相色谱法测定化妆品中9种对氨基苯甲酸及其酯类防晒剂[J].分析试验室,2014,33(9):1 108-1 113.
[15]MANE S,BRINGANS S,JOHNSON S,et al. Reversephase HPLC method for detection and quantification oflupin seedγ-conglutin[J]. J. Chromatogr. B,2017,1 063:123-129.
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[3]嵇正平,胡红果,颜丙义,等.电致化学发光法测定Mn2+量的研究[J].分析化学,2017,45(3):397-402.
[4]DONG Y P,WANG J,PENG Y,et al. Electrogeneratedchemiluminescence resonance energy transfer between lu-minol and Cd S/graphene nanocomposites and its sensingapplication[J].J.Electroanal.Chem.,2016,781:109-113.
[5]吴亮,沐春磊,张群林,等.纳米粒子参与的鲁米诺化学发光及其分析应用[J].化学进展,2013,25(7):1 187-1 197.
[6]YU D L,WANG P,ZHAO Y J,et al.Iodophenol blue-en-hanced luminol chemiluminescence and its application tohydrogen peroxide and glucose detection[J]. Talanta,2016,146:655-661.
[7]LIU H B,QIAO L,GAN N,et al.Electro-deposited poly-luminol molecularly imprinted polymer coating on carbox-yl graphene for stir bar sorptive extraction of estrogens inmilk[J].J.Chromatogr.B,2016,1 027:50-56.
[8]金茂俊,王静,杨丽华,等.化学发光免疫分析方法在食品安全检测中的研究进展[J].食品安全质量检测学报,2014,5(3):840-845.
[9]张云静,张群林.化学发光法在中药成分分析中的应用[J].中国药学杂志,2009,44(15):1 124-1 127.
[10]邵晓东,李瑛.鲁米诺化学发光分析法研究进展[J].化学研究,2010,21(1):102-112.
[11]魏峰,吴松,于晞,等.高效液相色谱法测定一次性卫生用纺织品中荧光增白剂[J].色谱,2015,33(2):158-163.
[12]LEGRAND T,RAKOTOSON M G,GALACTROS F,etal. Determination of hydroxyurea in human plasma byHPLC-UV using derivatization with xanthydrol[J]. J.Chromatogr.B,2017,1 064:85-91.
[13]高俊伟,叶平,谢坚,等.高效液相色谱-质谱法测定车用清洗剂中烷基酚聚氧乙烯醚[J].化学试剂,2018,40(1):57-60.
[14]张可冬,李慧勇,谭建华,等.高效液相色谱法测定化妆品中9种对氨基苯甲酸及其酯类防晒剂[J].分析试验室,2014,33(9):1 108-1 113.
[15]MANE S,BRINGANS S,JOHNSON S,et al. Reversephase HPLC method for detection and quantification oflupin seedγ-conglutin[J]. J. Chromatogr. B,2017,1 063:123-129.