3种微量元素对小球藻和小环藻生长的影响
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摘要
为研究3种微量元素(Fe、Co、Mo)对小球藻(Chlorella vulgaris)和小环藻(Cyclotella sp.)生长的影响,采用均匀设计的方法,在温度为25℃,光照强度为2 800 lx,光暗比为14 h∶10 h的条件下进行分组实验,测量不同实验组的藻细胞密度、平均生长速率和叶绿素a含量。结果表明:3种微量元素对小球藻和小环藻的各生长指标影响程度均不相同,其中Fe的影响最大。铁、钴和钼对小球藻的最大细胞密度和平均生长速率的影响顺序均为:Fe>Co>Mo,对小球藻的最大叶绿素a含量的影响顺序为:Fe>Mo>Co;对小环藻的最大细胞密度和平均生长速率的影响顺序均为:Fe>Mo>Co,对小环藻的最大叶绿素a含量的影响顺序为:Fe>Co>Mo。利用二次多项式逐步回归分析,得到3种微量元素与小球藻和小环藻的最大藻细胞密度、平均生长速率和最大叶绿素a含量间的显著有效且拟合度较高的二次回归方程,可用于估计特定条件下2种藻类的增殖。
To investigate the effects of three kinds of trace element(Fe, Co and Mo) on the growth of two species of algae, i.e, Chlorella vulgaris and Cyclotella sp., a grouping experiment was conducted using uniform-design method. The experimental condition was the temperature of 25 ℃, illumination intensity of 2 800 lx and the light to dark ratio being 14 h∶10 h; and the indexes measured for the testing groups included the cell density, the average growth rate and chlorophyll-a concentrations of C. vulgaris and Cyclotella sp. Result of the experiment showed that influences of three trace elements on the above growth indexes of Chlorella vulgaris and Cyclotella sp. differed;of the three elements, Fe influenced most, and the effects on the maximum cell density and the mean growth rate of Chlorella vulgaris ranked in descending order as Fe> Co> Mo; the effect on the maximum chlorophyll-a content ranked in the descending order as Fe> Mo> Co; the effects on the maximum cell density and mean growth rate of Cyclotella sp. were in the descending order as Fe> Mo> Co; and the effects on the maximum chlorophyll-a content in the descending order as Fe> Co> Mo. In addition, through the analysis by the quadratic polynomial stepwise regression the regression equations of high fitting degree were set up between the three trace elements and the growth parameters of Chlorella vulgaris and Cyclotella sp., which can be used to estimate the proliferation of two species of algae under the specific conditions.
To investigate the effects of three kinds of trace element(Fe, Co and Mo) on the growth of two species of algae, i.e, Chlorella vulgaris and Cyclotella sp., a grouping experiment was conducted using uniform-design method. The experimental condition was the temperature of 25 ℃, illumination intensity of 2 800 lx and the light to dark ratio being 14 h∶10 h; and the indexes measured for the testing groups included the cell density, the average growth rate and chlorophyll-a concentrations of C. vulgaris and Cyclotella sp. Result of the experiment showed that influences of three trace elements on the above growth indexes of Chlorella vulgaris and Cyclotella sp. differed;of the three elements, Fe influenced most, and the effects on the maximum cell density and the mean growth rate of Chlorella vulgaris ranked in descending order as Fe> Co> Mo; the effect on the maximum chlorophyll-a content ranked in the descending order as Fe> Mo> Co; the effects on the maximum cell density and mean growth rate of Cyclotella sp. were in the descending order as Fe> Mo> Co; and the effects on the maximum chlorophyll-a content in the descending order as Fe> Co> Mo. In addition, through the analysis by the quadratic polynomial stepwise regression the regression equations of high fitting degree were set up between the three trace elements and the growth parameters of Chlorella vulgaris and Cyclotella sp., which can be used to estimate the proliferation of two species of algae under the specific conditions.
引文
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[2]Hoffman R G,Lasater J A,Houk L W.Nutrient limitation on algal growth in the Eau Gallie River[J].Bulletin of Environmental Contamination and Toxicology,1974,12(5):587-593.
[3]赛音,敖登高娃.微量元素的环境化学及生物效应I微量元素在生物体内的功能[J].内蒙古石油化工,2001(1):18-21.
[4]李威,杨健,刘洪波,等.微量元素对水华发生、发展的影响[J].淡水渔业,2008(5):74-79.Li Wei,Yang Jian,Liu Hongbo,et al.Effects of trace elements on the occurrence and development of water-blooms[J].Freshwater Fisheries,2008(5):74-79.
[5]方开泰.均匀设计及其应用[J].数理统计与管理,1994(1):57-63,56.
[6]邱保胜,刘树文,戴国政.藻类利用微量金属元素的研究技术与方法[J].水生生物学报,2009,33(2):330-337.Qiu Baosheng,Liu Shuwen,Dai Guozheng.Methods and techniques for studying the utilization of trace metals by algae[J].Acta Hydroiologica Sinica,2009,33(2):330-337.
[7]陈宇炜,陈开宁,胡耀辉.浮游植物叶绿素a测定的“热乙醇法”及其测定误差的探讨[J].湖泊科学,2006(5):550-552.Chen Yuwei,Chen Kaining,Hu Yaohui.Discussion on possible error for phytoplankton chlorophyll-a concentration analysis using hot-ethanol extraction method[J].Lake Science,2006(5):550-552.
[8]Seaman Julia E,Seaman Christopher A,Allen I Elaine.So many variables,so few observations[J].Quality Progress,2013,46(9):34-41.
[9]袁志发,周静芋,郭满才,等.决策系数——通径分析中的决策指标[J].西北农林科技大学学报:自然科学版,2001(5):131-133.
[10]史绵红,胡雅琴,高连芬,等.金属营养元素与蓝藻的相互作用研究进展[J].地球与环境,2015,43(5):583-587.Shi Mianhong,Hu Yaqin,Gao Lianfen,et al.A review on the interactions between metal nutrients and cyanobacteria[J].Earth and Environment,2015,43(3):583-587.
[11]骆科枢.淡水藻类对磷、铁的协同吸收及增殖过程研究[D].广州:广东工业大学,2015.
[12]陈仕光,王志红.P和Fe、Mn、Zn对藻类生长影响的协同性[J].仲恺农业工程学院学报,2012,25(2):22-23,29.
[13]Zhang Yan,Gladyshev Vadim N.General trends in trace element utilization revealed by comparative genomic analyses of Co,Cu,Mo,Ni,and Se[J].JBC Papers in Press,2009,285(5):3393-3405.
[14]El-Sheekh MM,El-Naggar AH,Osman MEH,et al.Effect of cobalt on growth,pigments and the photosynthetic electron transport in Monoraphidiumminutum and Nitzchiaperminuta[J].Brazilian Journal of Plant Physiology,2003,15(3):159-166.
[15]Tiwari S,Mohanty P.Cobalt induced changes in photosystem activity in Synechocystis PCC 6803:alterations in energy distribution and stoichiometry[J].Photosynth Res,1996(50):243-256.
[16]García-Casal Maria N,Pereira Ana C,Leets Irene,et al.High iron content and bioavailability in humans from four species of marine algae[J].The Journal of Nutrition,2007,137(12):2691-2695.