藏药印度獐牙菜抗四氯化碳诱导的大鼠肝纤维化的作用
|
摘要
目的:观察印度獐牙菜对四氯化碳(CCl4)所致大鼠肝纤维化的防治作用。方法:32只SD大鼠随机分为空白组、模型组、印度獐牙菜组、阳性药组。除空白组外,各组大鼠腹腔注射40%CCl4/橄榄油溶液2m L/kg,每周2次,连续7周诱导肝纤维化。造模同时,给药组大鼠灌胃相应药物干预8周。实验结束后,观察肝组织病理学变化;测定血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅳ型胶原蛋白(ColⅣ)水平以及丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、总胆红素(TBIL)含量。结果:与空白组比较,模型组可见明显肝纤维化病理改变,AST、ALT、ALP和TBIL水平明显升高(P<0.05),HA、LN、PCⅢ和ColⅣ含量显著增高(P<0.01);与模型组比较,印度獐牙菜组肝纤维化病理程度减轻,肝功能指标改善明显,ALT、TBIL水平显著降低(P<0.05),AST、ALP水平呈下降趋势,HA、LN、PC及ColⅣ含量显著下降(P<0.01)。结论:印度獐牙菜具有较好的抗肝纤维化作用。
Objective: To observe the effects of Swertia chirayita on hepatic fibrosis model rats induced by carbon tetrachloride(CCl4). Methods: SD rats were randomly divided into the normal group, the model group, the Swertia chirayita group, and the positive drug group. All the groups except the normal group were intraperitoneally injected with 40%CCl4/olive oil solution 2m L/kg, twice a week for 7 consecutive weeks to induce hepatic fibrosis model. At the same time, rats in the treatment groups were given intragastric administration and the corresponding drugs were intervened for 8 weeks. Pathological changes of liver tissue were observed at the end of the experiment; the levels of serum hyaluronic acid(HA), laminin(LN), type three procollagen(PCⅢ) and type Ⅳ collagen(ColⅣ), and the content of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) and total bilirubin(TBIL) were detected. Results: Compared with the normal group, the pathological changes of liver fibrosis were obvious in the model group, and the levels of AST, ALT, ALP and TBIL were significantly increased(P<0.05), the contents of HA, LN, PC and ColⅣ increased significantly(P<0.01); compared with the model group, the pathological degree of liver fibrosis in Swertia chirayita group decreased, the liver function indexes improved obviously, and the levels of ALT and TBIL decreased significantly(P<0.05), the levels of AST and ALP showed a downward trend; the contents of HA, LN, PC and ColⅣ decreased significantly(P<0.01). Conclusion: Swertia chirayitahad reasonable effect on anti-hepatic fibrosis.
Objective: To observe the effects of Swertia chirayita on hepatic fibrosis model rats induced by carbon tetrachloride(CCl4). Methods: SD rats were randomly divided into the normal group, the model group, the Swertia chirayita group, and the positive drug group. All the groups except the normal group were intraperitoneally injected with 40%CCl4/olive oil solution 2m L/kg, twice a week for 7 consecutive weeks to induce hepatic fibrosis model. At the same time, rats in the treatment groups were given intragastric administration and the corresponding drugs were intervened for 8 weeks. Pathological changes of liver tissue were observed at the end of the experiment; the levels of serum hyaluronic acid(HA), laminin(LN), type three procollagen(PCⅢ) and type Ⅳ collagen(ColⅣ), and the content of alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) and total bilirubin(TBIL) were detected. Results: Compared with the normal group, the pathological changes of liver fibrosis were obvious in the model group, and the levels of AST, ALT, ALP and TBIL were significantly increased(P<0.05), the contents of HA, LN, PC and ColⅣ increased significantly(P<0.01); compared with the model group, the pathological degree of liver fibrosis in Swertia chirayita group decreased, the liver function indexes improved obviously, and the levels of ALT and TBIL decreased significantly(P<0.05), the levels of AST and ALP showed a downward trend; the contents of HA, LN, PC and ColⅣ decreased significantly(P<0.01). Conclusion: Swertia chirayitahad reasonable effect on anti-hepatic fibrosis.
引文
[1]Modol T,Brice N,Galarreta M,et al.Fibronectin peptides as potential regulators of hepatic fibrosis through apoptosis of hepatic stellate cells.J Cell Physiol,2015,30(3):546-549
[2]施亚军.肝纤维化中西医治疗进展.齐齐哈尔医学院学报,2012,33(23):3272-3274
[3]程明亮,周明玉.中医药防治肝纤维化.中华中医药杂志,2011,26(12):2761-2765
[4]高鹏飞,李凤贤,李辉,等.近期国内外应用天然药物防治肝纤维化的研究进展.中国中药杂志,2012,37(2):158-164
[5]黄苗,刘欣,董蕾,等.太白楤木对CCl4诱导肝纤维化大鼠的干预作用.中国中药杂志,2015,40(21):4251-4255
[6]王银辉,耿玲,李辉.灯盏乙素抗大鼠肝纤维化作用的研究.中国中药杂志,2015,40(10):1999-2003
[7]Phoboo S,Pinto Mda S,Barbosa A C.Phenolic-linked biochemical rationale for the anti-diabetic properties of Swertia chirayita(Roxb.ex Flem.)Karst.Phytother Res,2013,27(2):227-230
[8]施峰,黄一平,孙忠文.藏药印度獐牙菜的研究进展.中国药房,2010(19):1815-1817
[9]靳有才,魏立新,林蓉香,等.印度獐牙菜化学成分和药理作用研究进展.中国生化药物杂志,2012,31(60):431-434
[10]张宁,方衡,王雪,等.逍遥散对实验性肝纤维化大鼠模型干预作用的代谢组学研究.药物分析杂志,2014,4(4):588-594
[11]彭蕴茹,丁永芳,罗宇慧,等.藏药郎庆阿塔治疗肝纤维化的实验研究.中国实验方剂学杂志,2012,11(18):189-194
[12]白辰,车念聪,刘文兰,等.一贯煎对大鼠肝纤维化拮抗作用的影响.中华中医药杂志,2015,30(3):815-817
[13]王扬阳,周小航,孙晖,等.大鼠实验性肝纤维化动物模型的研究进展.黑龙江中医药,2013,42(3):62-63
[14]杨晴,颜迎春,张明香,等.血液指标在慢性乙型肝炎肝纤维化诊断中的价值.世界华人消化杂志,2015,23(15):2450-2452
[2]施亚军.肝纤维化中西医治疗进展.齐齐哈尔医学院学报,2012,33(23):3272-3274
[3]程明亮,周明玉.中医药防治肝纤维化.中华中医药杂志,2011,26(12):2761-2765
[4]高鹏飞,李凤贤,李辉,等.近期国内外应用天然药物防治肝纤维化的研究进展.中国中药杂志,2012,37(2):158-164
[5]黄苗,刘欣,董蕾,等.太白楤木对CCl4诱导肝纤维化大鼠的干预作用.中国中药杂志,2015,40(21):4251-4255
[6]王银辉,耿玲,李辉.灯盏乙素抗大鼠肝纤维化作用的研究.中国中药杂志,2015,40(10):1999-2003
[7]Phoboo S,Pinto Mda S,Barbosa A C.Phenolic-linked biochemical rationale for the anti-diabetic properties of Swertia chirayita(Roxb.ex Flem.)Karst.Phytother Res,2013,27(2):227-230
[8]施峰,黄一平,孙忠文.藏药印度獐牙菜的研究进展.中国药房,2010(19):1815-1817
[9]靳有才,魏立新,林蓉香,等.印度獐牙菜化学成分和药理作用研究进展.中国生化药物杂志,2012,31(60):431-434
[10]张宁,方衡,王雪,等.逍遥散对实验性肝纤维化大鼠模型干预作用的代谢组学研究.药物分析杂志,2014,4(4):588-594
[11]彭蕴茹,丁永芳,罗宇慧,等.藏药郎庆阿塔治疗肝纤维化的实验研究.中国实验方剂学杂志,2012,11(18):189-194
[12]白辰,车念聪,刘文兰,等.一贯煎对大鼠肝纤维化拮抗作用的影响.中华中医药杂志,2015,30(3):815-817
[13]王扬阳,周小航,孙晖,等.大鼠实验性肝纤维化动物模型的研究进展.黑龙江中医药,2013,42(3):62-63
[14]杨晴,颜迎春,张明香,等.血液指标在慢性乙型肝炎肝纤维化诊断中的价值.世界华人消化杂志,2015,23(15):2450-2452