蒙药那如-3指纹图谱的HPLC-DAD技术研究
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摘要
目的建立蒙药那如-3的HPLC指纹图谱。方法采用高效液相-二极管阵列检测(HPLC-DAD),色谱柱ODS-C18(250×4. 6 mm,5μm),流动相甲醇-0. 1%磷酸梯度洗脱,柱温30℃,流速0. 8 ml·min-1,检测波长为280,235,343 nm。结果通过10批次蒙药那如-3的化学成分指纹图谱分析,建立了蒙药那如-3的对照指纹图谱,通过"中药色谱指纹图谱相似度评价系统(2004A版本)",计算出10批次蒙药那如-3指纹图谱相似度均在0. 9以上,并以单味药材与蒙药那如-3的HPLC图谱进行比较,归属了主要色谱峰。结论以胡椒碱为参照峰,280 nm波长下标识了25个共有峰,建立了蒙药那如-3指纹图谱。此方法准确性好,可靠快捷,重复性好,初步确定了主要色谱峰的处方来源,为以后更好地全面控制复方药材质量提供科学依据。
Objective To establish HPLC fingerprinting of Mongolian medicine Na Ru-3. Methods HPLC-DAD,column ODS-C18( 250 × 4. 6 mm,5 m) and mobile phase methanol-0. 1% phosphoric acid were used for gradient elution. The column temperature was 30 ℃ and the flow rate was 0. 8 ml/min,detection wavelength was 280,235,343 nm. Results The control fingerprints of Mongolian medicine NaRu-3 were established by chemical composition fingerprinting analysis of 10 batches of Mongolian medicine NaRu-3. Ten fingerprints were obtained by " similarity evaluation system of chromatographic fingerprints of Chinese traditional medicine( 2004 A version) ". The similarities of the second Mongolian herbal medicine,NaRu-3 fingerprints,were all above 0. 9,and the main chromatographic peaks were attributed to the comparison of the HPLC profiles of the single herbs with those of Mongolian medicine. Conclusion Piperine was used as a reference peak,and 25 common peaks were identified at 280 nm wavelength. This method has good accuracy,reliability,quickness and good repeatability. The prescription source of the main chromatographic peaks was preliminarily determined to provide a scientific basis for better comprehensive control of the quality of compound medicinal materials in the future.
Objective To establish HPLC fingerprinting of Mongolian medicine Na Ru-3. Methods HPLC-DAD,column ODS-C18( 250 × 4. 6 mm,5 m) and mobile phase methanol-0. 1% phosphoric acid were used for gradient elution. The column temperature was 30 ℃ and the flow rate was 0. 8 ml/min,detection wavelength was 280,235,343 nm. Results The control fingerprints of Mongolian medicine NaRu-3 were established by chemical composition fingerprinting analysis of 10 batches of Mongolian medicine NaRu-3. Ten fingerprints were obtained by " similarity evaluation system of chromatographic fingerprints of Chinese traditional medicine( 2004 A version) ". The similarities of the second Mongolian herbal medicine,NaRu-3 fingerprints,were all above 0. 9,and the main chromatographic peaks were attributed to the comparison of the HPLC profiles of the single herbs with those of Mongolian medicine. Conclusion Piperine was used as a reference peak,and 25 common peaks were identified at 280 nm wavelength. This method has good accuracy,reliability,quickness and good repeatability. The prescription source of the main chromatographic peaks was preliminarily determined to provide a scientific basis for better comprehensive control of the quality of compound medicinal materials in the future.
引文
[1]邓广海,林华,龚又明.草乌及其炮制品中6种生物碱类成分的含量测定[J].中国实验方剂学杂志,2013,19(7):35.
[2]张秀娟,何丽娟,芦清,李东悦.民族药诃子药理活性研究进展[J].中国中药杂志,2016,41(4):619.
[3]李丹,杨异卉,赖睿智,等.荜茇化学成分和药理活性研究现状[J].中国临床药理学杂志,2017,33(6):565.
[4]中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准(蒙药分册)[S]. 1998.
[5] Yu F. Progress on the screening and analysis of bioactive compounds in traditional chinese medicines by biological fingerprinting analysis[J].Combinat Chem High Throughput Screening,2010,13(10):855.
[6]祝明,陈碧莲,,石上梅.中药指纹图谱技术在中国药典2015年版一部中的应用[J].中国现代应用药学,2016,33(5):611.
[7]佘一鸣,胡永慧,韩立云,刘素香,陈常青.中药质量控制的研究进展[J].中草药,2017,48(12):2557.
[8]刘东方,赵丽娜,李银峰,靳朝东.中药指纹图谱技术的研究进展及应用[J].中草药,2016,47(22):4085.
[9]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671.
[10]窦志华,许波,刘青青,等.茵陈蒿汤含药血清指纹图谱研究[J].中国药学杂志,2016,51(5):358.
[11]石朗.金银花UPLC指纹图谱及其次生代谢物化学成分比较研究[J].中医药信息,2017,34(4):26.
[2]张秀娟,何丽娟,芦清,李东悦.民族药诃子药理活性研究进展[J].中国中药杂志,2016,41(4):619.
[3]李丹,杨异卉,赖睿智,等.荜茇化学成分和药理活性研究现状[J].中国临床药理学杂志,2017,33(6):565.
[4]中华人民共和国卫生部药典委员会.中华人民共和国卫生部药品标准(蒙药分册)[S]. 1998.
[5] Yu F. Progress on the screening and analysis of bioactive compounds in traditional chinese medicines by biological fingerprinting analysis[J].Combinat Chem High Throughput Screening,2010,13(10):855.
[6]祝明,陈碧莲,,石上梅.中药指纹图谱技术在中国药典2015年版一部中的应用[J].中国现代应用药学,2016,33(5):611.
[7]佘一鸣,胡永慧,韩立云,刘素香,陈常青.中药质量控制的研究进展[J].中草药,2017,48(12):2557.
[8]刘东方,赵丽娜,李银峰,靳朝东.中药指纹图谱技术的研究进展及应用[J].中草药,2016,47(22):4085.
[9]国家药品监督管理局.中药注射剂指纹图谱研究的技术要求(暂行)[J].中成药,2000,22(10):671.
[10]窦志华,许波,刘青青,等.茵陈蒿汤含药血清指纹图谱研究[J].中国药学杂志,2016,51(5):358.
[11]石朗.金银花UPLC指纹图谱及其次生代谢物化学成分比较研究[J].中医药信息,2017,34(4):26.