鼻安纳米乳凝胶制备及其主要成分含量测定
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摘要
目的:研究鼻安纳米乳凝胶的制备及含量测定方法。方法:通过绘制伪三元相图及正交设计试验法优选纳米乳处方;采用气相色谱法测定鼻安纳米乳凝胶中薄荷脑和丁香酚含量。结果:最优的鼻安纳米乳处方为油相∶RH-40∶1,2-丙二醇=5∶12∶4。以泊洛沙姆407为凝胶基质成型性较优。薄荷脑、丁香酚分离度良好,薄荷脑在0.052～0.52 mg·mL~(-1)浓度范围内与其峰面积积分线性关系良好,平均回收率为98.5%;丁香酚在0.012 8～0.128 mg·mL~(-1)浓度范围内与其峰面积积分线性关系良好,平均回收率为97.7%。结论:鼻安纳米乳凝胶处方组成合理,制备工艺简单,含量测定方法可行。
Objective:To prepare and assay the Bian nanoemulsion-based gels. Methods:Optimize the formula composition of Bian nanoemulsion by pseudo-ternary phase diagram and orthogonal test.GC method was established to determine drug content in the gel.Results:The formula composition of Bian nanoemulsion was with the mass ratio of oil phase,Cremopher RH-40 and 1,2-propanediol of 5∶12∶4. Poloxamer 407 was used as gel matrix.Menthol and eugenol were separated well.Linear relationship of menthol was good in concentration range of 0.052～0.52 mg·mL~(-1) and average recovery was 98.5%.Linear relationship of eugenol was good in concentration range of 0.012 8～0.128 mg·mL~(-1) and average recovery was 97.7%.Conclusion:The technology of preparation is easy and method of assaying is feasible.
Objective:To prepare and assay the Bian nanoemulsion-based gels. Methods:Optimize the formula composition of Bian nanoemulsion by pseudo-ternary phase diagram and orthogonal test.GC method was established to determine drug content in the gel.Results:The formula composition of Bian nanoemulsion was with the mass ratio of oil phase,Cremopher RH-40 and 1,2-propanediol of 5∶12∶4. Poloxamer 407 was used as gel matrix.Menthol and eugenol were separated well.Linear relationship of menthol was good in concentration range of 0.052～0.52 mg·mL~(-1) and average recovery was 98.5%.Linear relationship of eugenol was good in concentration range of 0.012 8～0.128 mg·mL~(-1) and average recovery was 97.7%.Conclusion:The technology of preparation is easy and method of assaying is feasible.
引文
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[11]樊静静,袁玉霞,尹香阁,等.气相色谱法测定克疣纳米乳中冰片和薄荷脑含量[J].中国中医药信息杂志,2013,20(8):52-54.
[2]刘丽芳,张阳德.中药纳米乳研究进展[J].中医药导报,2010,16(8):112-115.
[3]李艳萍,张志荣.纳米乳的研究进展及其应用[J].华西药学杂志,2010,25(4):485-488.
[4]顾清,尤本明,杨帝顺,等.基于纳米乳-凝胶技术的雷公藤多苷纳米载体的制备及药效学研究[J].中国中药杂志,2015,40(1):73-78.
[5]吕长江,张蓉蓉,周军,等.冰片鸦胆子油纳米乳的制备及对大鼠脑胶质瘤的抑瘤作用研究[J].中国现代应用药学,2017,31(7):780-786.
[6]贺葵邦,王英姿,冯艾灵,等.苦参地龙纳米乳凝胶的制备及其离体透皮吸收研究[J].中国中药杂志,2013,38(16):2623-2627.
[7]赵惠茹,张鹏,刘少静,等.伪三元相图法研究芦丁自微乳化释药系统的制备工艺[J].中国药师,2016,19(7):1255-1259.
[8]赵宇,王晓波,张治然,等.温敏凝胶的研究与进展[J].中国药房,2015,26(1):132-135.
[9]姚娜,黄庆德.中药微乳凝胶剂的研究进展与应用[J].浙江中医药大学学报,2013,37(2):217-222.
[10]张伟,胡世强,陈娟,等.GC法测定肚痛健胃整肠丸中薄荷脑、愈创木酚和丁香酚[J].中成药,2013,35(2):286-289.
[11]樊静静,袁玉霞,尹香阁,等.气相色谱法测定克疣纳米乳中冰片和薄荷脑含量[J].中国中医药信息杂志,2013,20(8):52-54.