蛇毒精氨酸酯酶Agkihpin对人肝癌SMMC-7721细胞活力、增殖和迁移的影响
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摘要
目的:探讨蛇毒精氨酸酯酶Agkihpin对人肝癌SMMC-7721细胞活力、增殖和迁移的影响。方法:用不同浓度的Agkih-pin处理肝癌细胞株后,应用MTT法检测细胞活力,应用细胞计数法检测Agkihpin对细胞增殖和迁移的影响。结果:0.207～4.130mg/L浓度的Agkihpin作用SMMC-7721细胞后,与对照组比较,各加药组细胞活力明显下降(P<0.01),细胞活力随Agkihpin浓度增加而减弱(r=-0.81,P<0.05),细胞活力抑制率可达12.2%～46.2%;2.065～4.130mg/L剂量的Agki-hpin对人肝癌SMMC-7721细胞的增殖有明显的抑制作用(P<0.05),加药24,48h和72h后增殖抑制率分别为29.4%～88.2%、64.1%～84.6%和4.4%～30.5%,且剂量依赖作用明显(P<0.05);3.089mg/L和4.130mg/L剂量的Agkihpin对SMMC-7721细胞还有一定的杀伤作用,杀伤率分别为20.0%和79.6%;0.207～4.13 mg/L剂量的Agkihpin对人肝癌SMMC-7721细胞的迁移有明显的抑制作用(P<0.01),细胞迁移抑制率可达7.8%～98.0%,且剂量越大抑制作用越明显(r=0.841,P<0.05)。结论:一定剂量的Agkihpin可抑制人肝癌SMMC-7721细胞株的细胞活力、增殖和迁移。
Objective: To explore the impacts of Agkihpin on the cellular vitality,proliferation and migration of hepatocellular carcinoma SMMC-7721 cell strain.Methods: The cultured SMMC-7721 cells were treated with different concentrations of Agkihpin,then the cellular vitality was detected by MTT,the impacts on proliferation and migration of cell were detected by cell count.Results: Compared with the control group,the cellular vitality of SMMC-7721 was reduced obviously(P<0.01) with the increasing of concentration of Agkihpin(P<0.05,r=-0.81),and the inhibiting rate ranged from 12.2%-46.2% after the treatment of 0.207-4.130 mg/L Agkihpin;the proliferation of SMMC-7721 was inhibited obviously in a concentration-depended manner(P<0.05),and the inhibiting rate ranged from 29.4%-88.2%,64.1%-84.6% and 4.4%-30.5% respectively,after treated with 2.065-4.130 mg/L Agkihpin for 24 h,48 h and 72 h;the concentration of 3.089 and 4.130 mg/L Agkihpin can cause casualty of SMMC-7721 in a rate of 20.0% and 79.6%;the migration of SMMC-7721 was reduced obviously(P<0.01) with the increasing of concentration of Agkihpin(P<0.05,r=-0.841),and the inhibiting rate ranged from 7.28%-98.0%.Conclusion: Agkihpin can inhibit the cellular vitality,proliferation and migration of SMMC-7721 cells significantly.Agkihpin may work effectively in therapy and prevention of hepatocellular carcinoma in future.
Objective: To explore the impacts of Agkihpin on the cellular vitality,proliferation and migration of hepatocellular carcinoma SMMC-7721 cell strain.Methods: The cultured SMMC-7721 cells were treated with different concentrations of Agkihpin,then the cellular vitality was detected by MTT,the impacts on proliferation and migration of cell were detected by cell count.Results: Compared with the control group,the cellular vitality of SMMC-7721 was reduced obviously(P<0.01) with the increasing of concentration of Agkihpin(P<0.05,r=-0.81),and the inhibiting rate ranged from 12.2%-46.2% after the treatment of 0.207-4.130 mg/L Agkihpin;the proliferation of SMMC-7721 was inhibited obviously in a concentration-depended manner(P<0.05),and the inhibiting rate ranged from 29.4%-88.2%,64.1%-84.6% and 4.4%-30.5% respectively,after treated with 2.065-4.130 mg/L Agkihpin for 24 h,48 h and 72 h;the concentration of 3.089 and 4.130 mg/L Agkihpin can cause casualty of SMMC-7721 in a rate of 20.0% and 79.6%;the migration of SMMC-7721 was reduced obviously(P<0.01) with the increasing of concentration of Agkihpin(P<0.05,r=-0.841),and the inhibiting rate ranged from 7.28%-98.0%.Conclusion: Agkihpin can inhibit the cellular vitality,proliferation and migration of SMMC-7721 cells significantly.Agkihpin may work effectively in therapy and prevention of hepatocellular carcinoma in future.
引文
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[12]毕明俊,李琴,毕冬松.MRP-1及MRP-2在肝癌患者外周血和肝组织中表达及意义[J].中国普通外科杂志,2008,17(8):812-815.
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[14]陈东峰,赵峰.COX-2、MMP-2在直肠癌中的表达及其相关性分析[J].中华全科医学,2009,7(4):363-364,377.
[2]Stephen Swenson,Fritz Costa,Radu Minea,et al.Intra-venous liposomal delivery of the snake venom disinte-grin contortrostatin limits breast cancer progression[J].Pak J Physiol,2006,2(1):499-511.
[3]李振南,张小燕,芮景.蛇毒抗高凝状态酶对种植肝癌H22小鼠肿瘤抑制作用的研究[J].实用临床医药杂志,2007,11(3):45-48.
[4]胡启平,舒雨雁.江浙蝮蛇毒精氨酸酯酶Agkihpin的研究:Ⅰ、分离纯化和初步表征[J].四川动物,2006,25(2):251-256.
[5]胡启平,许淑茹,马军,等.蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系环氧合酶-2表达的影响研究[J].中国全科医学,2011,14(24):2761-2764.
[6]许淑茹,马军,袁志刚,等.蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系MRP1表达的影响[J].肿瘤防治研究,2011,38(7):731-735.
[7]许淑茹,马军,袁志刚,等.蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系E-钙粘素表达的影响[J].中华全科医学,2011,9(4):503-504,507.
[8]胡启平,舒雨雁.江浙蝮蛇毒精氨酸酯酶Agkihpin的研究:II.对鼻咽癌细胞活力、增殖、迁移和细胞形态的影响[J].四川动物,2006,25(2):257-260.
[9]胡启平,许淑茹,黄程新,等.蛇毒精氨酸酯酶Agkihpin抑制人肝癌SMMC-7721细胞株MRP1表达[J].中国肿瘤临床,2012,39(4):181-184.
[10]黄程新,许淑茹,胡启平,等.蛇毒精氨酸酯酶Agkihpin上调人肝癌SMMC-7721细胞株表皮钙粘素的表达研究[J].中国全科医学,2012,15(3C):1002-1005.
[11]陈清澄,刘广芬,王晴川.蝮蛇蛇毒柱层析分离及生化药理作用[J].福建医学院学报,1994,28(2):134-136.
[12]毕明俊,李琴,毕冬松.MRP-1及MRP-2在肝癌患者外周血和肝组织中表达及意义[J].中国普通外科杂志,2008,17(8):812-815.
[13]Konig J,Hartel M,Nies AT,et al.Expression and local-ization of human multidrug resistance protein(ABCC)family members in pancreatic carcinoma[J].Int J Canc-er,2005,15(3):359-367.
[14]陈东峰,赵峰.COX-2、MMP-2在直肠癌中的表达及其相关性分析[J].中华全科医学,2009,7(4):363-364,377.