贵州施秉云台山土壤有机磷细菌的解磷能力及生长状况
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摘要
【目的】为保护云台山喀斯特自然区生态环境及可持续发展提供依据。【方法】从云台山喀斯特自然区土壤中分离、纯化的有机磷细菌,分析其溶磷指数、磷酸酶活性、生长状况及碳源的利用率。【结果】从土壤分离的有机磷细菌(OPBS13、OPBS12、OPSB14和OPBS32)的溶磷指数、磷酸酶活性、生长状况和碳源的利用均存在显著差异;溶磷能力、磷酸酶活性以及生长状况均为OPSB14>OPBS32>OPBS12>OPBS13。【结论】贵州施秉云台山土壤有机磷细菌的解磷能力及生长状况与有机磷细菌分泌胞外磷酸酶活性有关,且受不同碳源影响。
【Objective】This paper aims to provide references for eco-environment protection and sustainable development of Yuntaishan Karst natural area. 【Method】The phosphate-solubilizing index,phosphatase activity,growth status and carbon source utilization were analyzed by isolating and purifying organophosphate bacteria from its soil. 【Result】The phosphorus uptake,phosphatase activity,growth status and carbon source efficiency of organic phosphorus bacteria( OPBS13,OPBS12,OPSB14,OPBS32) isolated from soil were different and the phospholytic activity,phosphatase activity and growth rate were as follows: OPSB14 > OPBS32 > OPBS12 > OPBS13.【Conclusion】Phosphorussolubilizing ability and growth status of organophosphorus bacteria in Yuntai mountain,Shibin,Guizhou province were related to the secretion of extracellular phosphatase by organophosphorus bacteria and were affected by the utilization of different carbon sources.
【Objective】This paper aims to provide references for eco-environment protection and sustainable development of Yuntaishan Karst natural area. 【Method】The phosphate-solubilizing index,phosphatase activity,growth status and carbon source utilization were analyzed by isolating and purifying organophosphate bacteria from its soil. 【Result】The phosphorus uptake,phosphatase activity,growth status and carbon source efficiency of organic phosphorus bacteria( OPBS13,OPBS12,OPSB14,OPBS32) isolated from soil were different and the phospholytic activity,phosphatase activity and growth rate were as follows: OPSB14 > OPBS32 > OPBS12 > OPBS13.【Conclusion】Phosphorussolubilizing ability and growth status of organophosphorus bacteria in Yuntai mountain,Shibin,Guizhou province were related to the secretion of extracellular phosphatase by organophosphorus bacteria and were affected by the utilization of different carbon sources.
引文
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[15]刘晓伟,王火焰,周健民,等.供氮浓度与时期对水稻产量及氮吸收的影响[J].江苏农业科学,2017,45(7):66-69.
[16]米娜娜,贾蓉,贾雪雪,等.生物炭对水稻土Fe(Ⅲ)还原过程中碳源利用效率的影响[J].水土保持学报,2015,29(3):144-149.
[2]刘丛强,郎赟超,李思亮,等.喀斯特生态系统生物地球化学过程与物质循环研究:重要性、现状与趋势[J].地学前缘,2009(6):1-12.
[3]刘方,王世杰,刘元生,等.喀斯特石漠化过程土壤质量变化及生态环境影响评价[J].生态学报,2005(3):639-644.
[4]贺红早,张珍明,何云松,等.贵州省云台山喀斯特森林土壤性状特征研究[J].湖北农业科学,2013,52(13):3007-3010.
[5]罗海波,蒲通达,陈祖拥.贵州南部喀斯特植被群落变化对小生境土壤养分的影响[J].贵州农业科学,2010,38(6):112-115.
[6]刘成刚,薛建.喀斯特石漠化山地不同类型人工林土壤的基本性质和综合评价[J].植物生态学报,2011,35(10):1050-1060.
[7]张超,朱三荣,田峰,等.不同绿肥对湘西烟田土壤细菌群落结构与多样性的影响[J].贵州农业科学,2016,44(5):43-46.
[8]杨旭,曾庆春,白涛,等.采石场废弃地不同植被恢复模式对土壤微生物数量的影响[J].贵州农业科学,2016,44(6):167-171.
[9]贺红早,张珍明,刘盈盈,等.贵州云台山喀斯特森林土壤有机碳及黑碳分布特征[J].贵州农业科学,2013,41(5):90-92.
[10]Fyllas N M,Patiňo S,Baker T R,et al.Basin-wide variations in foliar properties of Amazonian forest:physiology,soils and climate[J].Biogeosciences,2009,6:2677-2708.
[11]Elser J J,Bracken M E S,Cleland E E,et al.Global analysis of nitrogen and phosphorus limitation of primary producers in freshwater,marine and terrestrial ecosystems[J].Ecology Letters,2007,10:1135-1142.
[12]周福健,严友进,戴全厚,等.喀斯特石漠化治理措施的土壤养分响应——以贵州省六枝特区为例[J].江苏农业科学,2016,44(5):469-472.
[13]Richardson S J,Allen R B,Doherty J E.Shifts in leaf N:P ratio during resorption reflect soil P in temperate rainforest[J].Functional Ecology,2008,22,738-745.
[14]郑红丽,周晓荣,樊明寿.燕麦根际有机磷细菌的分离及其有关生理特性的研究[J].干旱地区农业研究,2007,25(5):1-4.
[15]刘晓伟,王火焰,周健民,等.供氮浓度与时期对水稻产量及氮吸收的影响[J].江苏农业科学,2017,45(7):66-69.
[16]米娜娜,贾蓉,贾雪雪,等.生物炭对水稻土Fe(Ⅲ)还原过程中碳源利用效率的影响[J].水土保持学报,2015,29(3):144-149.