黄花蒿浸提液对2种蔬菜种子萌发及幼苗生长的化感效应
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摘要
【目的】探明土壤连作障碍的变化规律,为黄花蒿的友好栽培及其次生代谢组分的进一步开发利用提供依据。【方法】采用黄花蒿根、茎、叶不同浓度浸提液处理小白菜和萝卜种子,探讨其种子萌发率、幼苗生长、过氧化物酶(POD)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性,以及丙二醛(MDA)含量的变化。【结果】黄花蒿根、茎、叶不同浓度浸提液处理,对小白菜和萝卜种子的发芽率和幼苗生长均产生明显抑制作用,且随处理浓度增加,对其种子萌发的抑制率越大;黄花蒿根、茎、叶不同浓度浸提液处理,对小白菜和萝卜幼苗生长的化感效应存在差异,对其幼苗叶片的POD、SOD和CAT活性具有浓度效应,而MDA含量随黄花蒿浸提液浓度增大而增加。【结论】黄花蒿根、茎、叶水浸提液中含有化感抑制物质,其化感效应超过小白菜和萝卜体内POD、SOD和CAT活性的调节能力,幼苗体内膜脂过氧化程度加重。
【Objective】The present paper aimed to verify the variation regulation of soil successive cropping obstacle and provide the scientific basis for further exploitation and utilization of Artemisia annua environment-friendly cultivation and its secondary metabolism components.【Method】 The seed germination rate, seedling growth, leaf POD, SOD and CAT activity and leaf MDA content of Raphanus sativus and Brassica chinensis seeds treated with different extracts with different concentration from Artemisia annua roots, stems and leaves were discussed.【Result】The different extracts with different concentration from Artemisia annua roots, stems and leaves all have the significant inhibition effect on seed germination rate and seedling growth of Raphanus sativus and Brassica chinensis and the inhibition effect of Artemisia annua extracts on seed germination of Raphanus sativus and Brassica chinensis rises with increase of the extract concentration. There is a difference in allelopathic effect of different extracts with different concentration from Artemisia annua roots, stems and leaves on growth of Raphanus sativus and Brassica chinensis seedlings. The different extracts with different concentration from Artemisia annua roots, stems and leaves have the concentration effect on leaf POD, SOD and CAT activity of Raphanus sativus and Brassica chinensis seedlings but the leaf MDA content rises with increase of Artemisia annua extracts concentration.【Conclusion】The allelopthic effect of the allelopathic inhibition substance in the extracts from Artemisia annua roots, stems and leaves exceeds the adjusting ability of POD, SOD and CAT activity in Raphanus sativus and Brassica chinensis, which increases in vivo membrane lipid peroxide level of Raphanus sativus and Brassica chinensis.
【Objective】The present paper aimed to verify the variation regulation of soil successive cropping obstacle and provide the scientific basis for further exploitation and utilization of Artemisia annua environment-friendly cultivation and its secondary metabolism components.【Method】 The seed germination rate, seedling growth, leaf POD, SOD and CAT activity and leaf MDA content of Raphanus sativus and Brassica chinensis seeds treated with different extracts with different concentration from Artemisia annua roots, stems and leaves were discussed.【Result】The different extracts with different concentration from Artemisia annua roots, stems and leaves all have the significant inhibition effect on seed germination rate and seedling growth of Raphanus sativus and Brassica chinensis and the inhibition effect of Artemisia annua extracts on seed germination of Raphanus sativus and Brassica chinensis rises with increase of the extract concentration. There is a difference in allelopathic effect of different extracts with different concentration from Artemisia annua roots, stems and leaves on growth of Raphanus sativus and Brassica chinensis seedlings. The different extracts with different concentration from Artemisia annua roots, stems and leaves have the concentration effect on leaf POD, SOD and CAT activity of Raphanus sativus and Brassica chinensis seedlings but the leaf MDA content rises with increase of Artemisia annua extracts concentration.【Conclusion】The allelopthic effect of the allelopathic inhibition substance in the extracts from Artemisia annua roots, stems and leaves exceeds the adjusting ability of POD, SOD and CAT activity in Raphanus sativus and Brassica chinensis, which increases in vivo membrane lipid peroxide level of Raphanus sativus and Brassica chinensis.
引文
[1]WANG D L.The review of rice allelopathy[J].Acta Ecological Sinica,1998,18(3):326-335(in Chinese).
[2]LIN W, HUAQIN H E, GUO Y, et al.Rice allelopathy and its physiobiochemical characteristics[J].Chinese Journal of Applied Ecology, 2001,12:871-875.
[3]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:198.
[4]DELABAYS N, SLACANIN I, BOHREN C.Herbicidal potential of artemisinin and allelopathic properties of Artemisia annua L: From the laboratory to the field[J].Journal of Plant Diseases & Proctection Supplement, 2008(21):317-322.
[5]王硕,慕小倩,杨超,等.黄花蒿浸提液对小麦幼苗的化感作用及其机理研究[J].西北农林科技大学学报(自然科学版),2016,34(6):106-110.
[6]钟国跃,凌云.黄花蒿优质种质资源的研究[J].中草药,1998(4):264-267.
[7]张志忠,石秋香,孙志浩,等.入侵植物空心莲子草对生菜和萝卜的化感效应[J].草业学报,2013,22(1):288-293.
[8]高阳,朱双丽,吴庆珊,等.金钗石斛 IAA 内生细菌筛选及对干旱胁迫下玉米种子萌发的影响[J].种子,2017,36(4):36-40.
[9]曲敏,秦丽楠,刘羽佳,等.两种检测SOD酶活性方法的比较[J].食品安全质量检测学报,2014(10):3318-3323.
[10]陈晓敏.测定切花中过氧化氢酶活性的3种常用方法的比较[J].热带农业科学,2002,22(5):13-16.
[11]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008(2):323-324.
[12]王以柔,刘鸿先,李平,等.在光和黑暗条件下低温对水稻幼苗光合器官膜脂过氧化物作用的影响[J].植物生理学报,1986(12):244-251.
[13]LIN W X, KIM K U, SHIN D H.Rice allelopathic potential and its modes of action on Barnyardgrass(Echinochloa crusgalli)[J]. Allelopathy Journal, 2000,7(2):215-224.
[14]DAYAN F E, ROMAGNI J G, DUKE S O.Investigating the mode of action of natural phytotoxins[J].Journal of Chemical Ecology, 2000, 26(9):2079-2094.
[15]MAIGHANY F, KHALGHANI J, BAGHESTANI M A, et al.Allelopathic potential of Trifolium resupinatum L.(Persian clover)and Trifolium alexandrium L.(Berseem clover)[J].Weed Biology & Management, 2007,7(3):178-183.
[16]周凯,郭维明,徐迎春.菊科植物化感作用研究进展[J].生态学报,2004,24(8):1776-1784.
[17]任艳萍,江莎,古松,等.外来植物黄顶菊根、茎、叶的化感作用初探[J].植物保护,2009,35(3):36-40.
[18]颜红果,唐露,袁珂,等.羽芒菊对4种农作物的化感作用研究[J].中草药,2014,45(12):1780-1784.
[19]EFFERTH T, HERRMANN F, TAHRANI A, et al.Cytotoxic activity of secondary metabolites derived from Artemisia annua L.towards cancer cells in comparison to its designated active constituent artemisinin[J].Phytomedicine International Journal of Phytotherapy & Phytopharmacology, 2011,18(11):959-969.
[20]PANDEY N, PANDEYRAI S.Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L.against short-term UV-B radiation[J].Planta, 2014,240(3):611-627.
[21]WANG H H, MA C F, LI Z Q, et al.Effects of exogenous methyl jasmonate on artemisinin biosynthesis and secondary metabolites in Artemisia annua L.[J].Industrial Crops & Products, 2010,31(2):214-218.
[22]白祯,黄玥,黄建国.青蒿素对蔬菜种子发芽和幼苗生长的化感效应[J].生态学报,2013,33(23):7576-7582.
[23]张亚琴,陈雨,雷飞益,等.药用植物化感自毒作用研究进展[J].中草药,2018,49(8):1946-1956.
[2]LIN W, HUAQIN H E, GUO Y, et al.Rice allelopathy and its physiobiochemical characteristics[J].Chinese Journal of Applied Ecology, 2001,12:871-875.
[3]国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015:198.
[4]DELABAYS N, SLACANIN I, BOHREN C.Herbicidal potential of artemisinin and allelopathic properties of Artemisia annua L: From the laboratory to the field[J].Journal of Plant Diseases & Proctection Supplement, 2008(21):317-322.
[5]王硕,慕小倩,杨超,等.黄花蒿浸提液对小麦幼苗的化感作用及其机理研究[J].西北农林科技大学学报(自然科学版),2016,34(6):106-110.
[6]钟国跃,凌云.黄花蒿优质种质资源的研究[J].中草药,1998(4):264-267.
[7]张志忠,石秋香,孙志浩,等.入侵植物空心莲子草对生菜和萝卜的化感效应[J].草业学报,2013,22(1):288-293.
[8]高阳,朱双丽,吴庆珊,等.金钗石斛 IAA 内生细菌筛选及对干旱胁迫下玉米种子萌发的影响[J].种子,2017,36(4):36-40.
[9]曲敏,秦丽楠,刘羽佳,等.两种检测SOD酶活性方法的比较[J].食品安全质量检测学报,2014(10):3318-3323.
[10]陈晓敏.测定切花中过氧化氢酶活性的3种常用方法的比较[J].热带农业科学,2002,22(5):13-16.
[11]李忠光,龚明.愈创木酚法测定植物过氧化物酶活性的改进[J].植物生理学通讯,2008(2):323-324.
[12]王以柔,刘鸿先,李平,等.在光和黑暗条件下低温对水稻幼苗光合器官膜脂过氧化物作用的影响[J].植物生理学报,1986(12):244-251.
[13]LIN W X, KIM K U, SHIN D H.Rice allelopathic potential and its modes of action on Barnyardgrass(Echinochloa crusgalli)[J]. Allelopathy Journal, 2000,7(2):215-224.
[14]DAYAN F E, ROMAGNI J G, DUKE S O.Investigating the mode of action of natural phytotoxins[J].Journal of Chemical Ecology, 2000, 26(9):2079-2094.
[15]MAIGHANY F, KHALGHANI J, BAGHESTANI M A, et al.Allelopathic potential of Trifolium resupinatum L.(Persian clover)and Trifolium alexandrium L.(Berseem clover)[J].Weed Biology & Management, 2007,7(3):178-183.
[16]周凯,郭维明,徐迎春.菊科植物化感作用研究进展[J].生态学报,2004,24(8):1776-1784.
[17]任艳萍,江莎,古松,等.外来植物黄顶菊根、茎、叶的化感作用初探[J].植物保护,2009,35(3):36-40.
[18]颜红果,唐露,袁珂,等.羽芒菊对4种农作物的化感作用研究[J].中草药,2014,45(12):1780-1784.
[19]EFFERTH T, HERRMANN F, TAHRANI A, et al.Cytotoxic activity of secondary metabolites derived from Artemisia annua L.towards cancer cells in comparison to its designated active constituent artemisinin[J].Phytomedicine International Journal of Phytotherapy & Phytopharmacology, 2011,18(11):959-969.
[20]PANDEY N, PANDEYRAI S.Modulations of physiological responses and possible involvement of defense-related secondary metabolites in acclimation of Artemisia annua L.against short-term UV-B radiation[J].Planta, 2014,240(3):611-627.
[21]WANG H H, MA C F, LI Z Q, et al.Effects of exogenous methyl jasmonate on artemisinin biosynthesis and secondary metabolites in Artemisia annua L.[J].Industrial Crops & Products, 2010,31(2):214-218.
[22]白祯,黄玥,黄建国.青蒿素对蔬菜种子发芽和幼苗生长的化感效应[J].生态学报,2013,33(23):7576-7582.
[23]张亚琴,陈雨,雷飞益,等.药用植物化感自毒作用研究进展[J].中草药,2018,49(8):1946-1956.