宽筋藤化学成分的研究
|
摘要
目的:研究宽筋藤的化学成分。方法:采用硅胶柱层析、反相硅胶柱层析及半制备HPLC等色谱技术进行分离纯化,采用现代波谱技术鉴定化合物结构。结果:从宽筋藤中分离得到12个化合物,分别鉴定为:环-(缬-酪)二肽(1)、环-(异亮-丙)二肽(2)、酪氨酸(3)、脯氨酸(4)、苯甲醇-O-β-D-吡喃葡萄糖苷(5)、4,4′-dimethoxy-3′-hydroxy-7,9′:7,9′-diepoxylignan-3-O-D-glucopyranoside(6)、丁香脂素-4′-O-β-葡萄糖苷(7)、tinosinen(8)、tinosineside B(9)、槲皮素(10)、芦丁(11)、异鼠李素(12)。结论:其中,化合物1～6为首次从该植物中分离得到。
Objective:To study the chemical constituents from the stems of Tinospora sinensis.Methods:The compounds were isolated and purified by repeated column chromatographies with silica gel and ODS columns,and semi-preparative HPLC.Their structures were identified by spectroscopic data.Results:Twelve compounds were isolated from the Tinospora sinensis,which were identified as cyclo-(Val-Tyr)(1),cyclo-(Ile-Ala)(2),tyrosine(3),proline(4),benzyl-O-β-D-glucopyranoside(5),4,4′-dimethoxy-3′-hydroxy-7,9′:7,9′-diepoxylignan-3-O-D-glucopyranoside(6),syringaresinol-4′-O-β-glucopyranoside(7),tinosinen(8),tinosineside B(9),quercetin(10),rutin(11),isorhamnetin(12).Conclusion:Compounds 1～6 are isolated from this plant for the first time.
Objective:To study the chemical constituents from the stems of Tinospora sinensis.Methods:The compounds were isolated and purified by repeated column chromatographies with silica gel and ODS columns,and semi-preparative HPLC.Their structures were identified by spectroscopic data.Results:Twelve compounds were isolated from the Tinospora sinensis,which were identified as cyclo-(Val-Tyr)(1),cyclo-(Ile-Ala)(2),tyrosine(3),proline(4),benzyl-O-β-D-glucopyranoside(5),4,4′-dimethoxy-3′-hydroxy-7,9′:7,9′-diepoxylignan-3-O-D-glucopyranoside(6),syringaresinol-4′-O-β-glucopyranoside(7),tinosinen(8),tinosineside B(9),quercetin(10),rutin(11),isorhamnetin(12).Conclusion:Compounds 1～6 are isolated from this plant for the first time.
引文
[1]湖南省食品药品监督管理局.湖南省中药材标准[S].长沙:湖南科学技术出版社,2010.
[2]西藏,青海,四川,甘肃,云南,新疆卫生局.藏药标准[S].西宁:青海人民出版社,1979.
[3]白文婷,谢一辉,韦益飞,等.藏药宽筋藤的化学成分及其药理作用研究进展[J].中国现代中药,2016,18(12):1666-1669.
[4]And TS,Thomas H.Structures,sensory acticity,and dose-response,functions of 2,5-diketopiperazines in roasted cocoa nibs[J].Journal of Agricultural & Food Chemistry,2005,53(18):7222-7231.
[5]Wang S,Tan N,Yang Y,et al.Cyclodipeptides from the roots of Panax notoginseng[J].Natural Product Research and Development,2004,16(5):383-386.
[6]Nilsson M,Duarte IF,Almeida C,et al.High-resolution NMR and diffusion-ordered spectroscopy of port wine[J].Journal of Agricultural & Food Chemistry,2004,52(12):3736-3743.
[7]Tian J,Yin Y.Novel phenomena in the 13C-NMR spectra of amino acids[J].Amino Acids,2004,26(2):175-181.
[8]周佳,周先丽,梁成钦,等.铁皮石斛化学成分研究[J].中草药,2015,46(9):1292-1295.
[9]Li N,Tan NH,Zhou J.A new lignan glycoside from Curculigo capitulate[J].Acta Botanica Yunnanica,2003,25(6):711-715.
[10]Ando H,Hirai Y,Fujii M,et al.The chemical constituents of fresh Gentian Root[J].Journal of Natural Medicines,2007,61:269-279.
[11]Yonemitsu M,Fukuda N,Kimura T.Studies on the constituents of Tinospora sinensis:Ⅰ.separation and structure of the new phenolic glycoside Tinosinen[J].Planta Medica,1993,59:552-553.
[12]Yonemitsu M,Fukuda N,Kimura T,et al.Isolation and structure elucidation of two new dinorditerpene glucosides,Tinosineside A and B[J].Europea Journal of Organic Chemistry,1995,1995(2):437-439.
[13]Zeng LM,Wang CJ,Su JY,et al.Flavonoids from the red alga Acanthophora spicifera[J].Chinese Journal of Chemistry,2001,19(11):1097-1100.
[14]艾尼瓦尔·艾克木,古丽·买买提祖农,李建光,等.新疆传统维药黑种草子的化学成分研究[J].上海中医药杂志,2008,42(9):73-75.
[15]雷军,肖云川,王文静,等.糯米藤中黄酮类化学成分研究[J].中国中药杂志,2012,37(4):478-482.
[2]西藏,青海,四川,甘肃,云南,新疆卫生局.藏药标准[S].西宁:青海人民出版社,1979.
[3]白文婷,谢一辉,韦益飞,等.藏药宽筋藤的化学成分及其药理作用研究进展[J].中国现代中药,2016,18(12):1666-1669.
[4]And TS,Thomas H.Structures,sensory acticity,and dose-response,functions of 2,5-diketopiperazines in roasted cocoa nibs[J].Journal of Agricultural & Food Chemistry,2005,53(18):7222-7231.
[5]Wang S,Tan N,Yang Y,et al.Cyclodipeptides from the roots of Panax notoginseng[J].Natural Product Research and Development,2004,16(5):383-386.
[6]Nilsson M,Duarte IF,Almeida C,et al.High-resolution NMR and diffusion-ordered spectroscopy of port wine[J].Journal of Agricultural & Food Chemistry,2004,52(12):3736-3743.
[7]Tian J,Yin Y.Novel phenomena in the 13C-NMR spectra of amino acids[J].Amino Acids,2004,26(2):175-181.
[8]周佳,周先丽,梁成钦,等.铁皮石斛化学成分研究[J].中草药,2015,46(9):1292-1295.
[9]Li N,Tan NH,Zhou J.A new lignan glycoside from Curculigo capitulate[J].Acta Botanica Yunnanica,2003,25(6):711-715.
[10]Ando H,Hirai Y,Fujii M,et al.The chemical constituents of fresh Gentian Root[J].Journal of Natural Medicines,2007,61:269-279.
[11]Yonemitsu M,Fukuda N,Kimura T.Studies on the constituents of Tinospora sinensis:Ⅰ.separation and structure of the new phenolic glycoside Tinosinen[J].Planta Medica,1993,59:552-553.
[12]Yonemitsu M,Fukuda N,Kimura T,et al.Isolation and structure elucidation of two new dinorditerpene glucosides,Tinosineside A and B[J].Europea Journal of Organic Chemistry,1995,1995(2):437-439.
[13]Zeng LM,Wang CJ,Su JY,et al.Flavonoids from the red alga Acanthophora spicifera[J].Chinese Journal of Chemistry,2001,19(11):1097-1100.
[14]艾尼瓦尔·艾克木,古丽·买买提祖农,李建光,等.新疆传统维药黑种草子的化学成分研究[J].上海中医药杂志,2008,42(9):73-75.
[15]雷军,肖云川,王文静,等.糯米藤中黄酮类化学成分研究[J].中国中药杂志,2012,37(4):478-482.