不同产地杜仲根际土真菌群落结构的差异性分析
|
摘要
基于宏条码技术对采自贵州遵义县、湖南慈利县和四川旺苍县3个杜仲产地根际土壤的真菌群落组成进行了分析。研究获得根际土壤真菌有效序列共10 775条,聚类后OTUs对应的真菌类群涉及子囊菌门Ascomycota、担子菌门Basidiomycota、接合菌门Zygomycota、球囊菌门Glomeromycota、壶菌门Chytridiomycota、类原生动物门Rozellomycota的22纲、59目、103科、108个属的550个真菌分类单元(OTUs)。在属的水平上,湖南慈利样本(ECS)有119个属,优势属为镰刀菌属Fusarium,相对丰度为16.31%,其次是被孢霉属Mortierella(14.67%)、毛壳菌科Chaetomiaceae一未定属(13.01%)和木霉属Trichoderma(11.37%)等。四川旺苍样本(EWS)共有116个属,优势属为丝齿菌属Hyphodontia,相对丰度32.32%,其次为镰刀菌Fusarium(13.41%)等。贵州遵义样本(EZS)有110个属,优势属为被孢霉属Mortierella,相对丰度30.74%,其次是木霉属Trichoderma(25.96%)和镰刀菌属Fusarium(4.85%)等。α多样性分析表明,不同产地杜仲根际土壤真菌群落组成有明显差异。遵义样本(EZS)、慈利样本(ECS)和旺苍样本(EWS)的Shannon多样性指数从高到低为:ECS(2.7661)> EZS(2.4841)>EWS(2.1326)。不同杜仲产区土壤理化因子及4个主要活性成分(松脂醇二葡萄糖苷、绿原酸、京尼平苷酸和京尼平苷)与根际土真菌群落组成的相关性分析表明,4个主要活性成分皆为四川旺苍(EWS)产地含量最高,其次是湖南慈利,贵州遵义最低甚至不含京尼平苷。杜仲根际土壤样品真菌物种组成丰富,不同地区杜仲根际土壤真菌群落组成、分类单元的相对丰度及优势分类单元皆存在不同程度差异。杜仲根际土真菌群落结构的多样性与土壤理化因子及杜仲主要有效成分含量具有一定相关性。杜仲根际土真菌群落组成的结构谱是杜仲道地性的重要特征。
Based on macrobarcode technology, the fungal community compositions of the rhizosphere soil of Eucommia ulmoides in three producing areas, Zunyi County of Guizhou Province, Cili County of Hunan Province and Wangcang County of Sichuan Province, were analyzed. A total of 10 775 effective sequences of rhizospheric fungi were obtained. The corresponding fungal group of OTUs after clustering belonged to six phyla(Ascomycota, Basidiomycota, Zygomycota, Glomeromycota, Chytridiomycota, Rozellomycota), 22 classes, 59 orders, 103 families, 108 genera and 550 fungal taxa(OTUs). At the generic level, 119 genera were found in samples from Cili Hunan, and the dominant genus was Fusarium with relative abundance of 16.31%, followed by Mortierella(14.67%), an unclassified genus of Chaetomiaceae(13.01%), Trichoderma(11.37%) and so on.There were 116 genera in samples from Wangcang of Sichuan, and the dominant genus was Hyphodontia with relative abundance of 32.32%, followed by Fusarium(13.41%). The samples from Zunyi in Guizhou yielded 110 genera, and the dominant genus was Mortierella with relative abundance of 30.74%, followed by Trichoderma(25.96%) and Fusarium(4.85%). The alpha diversity in rhizosphere soil showed significant differences in the composition of fungal community of Eucommia ulmoides rhizosphere in different habitats. The Shannon indexes of the three samples were arranged from high to low in order of ECS(2.7661) > EZS(2.4841) > EWS(2.1326). Analysis of correlation of the soil physicochemical factors and four main active compounds, rosinol pinoresinol diglucoside, chlorogenic acid, geniposide and geniposide acid, with fungal community showed that EWS has the highest content of active compounds, followed by ECS, and EZS was the lowest and even absence of geniposide. In conclusion, the composition of fungal community, the relative abundance of species and the dominant taxa varied in the rhizosphere soil of Eucommia ulmoides from different regions and was related to soil physicochemical factors and the main active compounds. The structural spectrum of the fungal community composition in the rhizosphere soil is of important feature of geoherbalism of Eucommia ulmoides.
Based on macrobarcode technology, the fungal community compositions of the rhizosphere soil of Eucommia ulmoides in three producing areas, Zunyi County of Guizhou Province, Cili County of Hunan Province and Wangcang County of Sichuan Province, were analyzed. A total of 10 775 effective sequences of rhizospheric fungi were obtained. The corresponding fungal group of OTUs after clustering belonged to six phyla(Ascomycota, Basidiomycota, Zygomycota, Glomeromycota, Chytridiomycota, Rozellomycota), 22 classes, 59 orders, 103 families, 108 genera and 550 fungal taxa(OTUs). At the generic level, 119 genera were found in samples from Cili Hunan, and the dominant genus was Fusarium with relative abundance of 16.31%, followed by Mortierella(14.67%), an unclassified genus of Chaetomiaceae(13.01%), Trichoderma(11.37%) and so on.There were 116 genera in samples from Wangcang of Sichuan, and the dominant genus was Hyphodontia with relative abundance of 32.32%, followed by Fusarium(13.41%). The samples from Zunyi in Guizhou yielded 110 genera, and the dominant genus was Mortierella with relative abundance of 30.74%, followed by Trichoderma(25.96%) and Fusarium(4.85%). The alpha diversity in rhizosphere soil showed significant differences in the composition of fungal community of Eucommia ulmoides rhizosphere in different habitats. The Shannon indexes of the three samples were arranged from high to low in order of ECS(2.7661) > EZS(2.4841) > EWS(2.1326). Analysis of correlation of the soil physicochemical factors and four main active compounds, rosinol pinoresinol diglucoside, chlorogenic acid, geniposide and geniposide acid, with fungal community showed that EWS has the highest content of active compounds, followed by ECS, and EZS was the lowest and even absence of geniposide. In conclusion, the composition of fungal community, the relative abundance of species and the dominant taxa varied in the rhizosphere soil of Eucommia ulmoides from different regions and was related to soil physicochemical factors and the main active compounds. The structural spectrum of the fungal community composition in the rhizosphere soil is of important feature of geoherbalism of Eucommia ulmoides.
引文
Butler JL,Williams MA,Bottomley PJ,Myrold DD,2003.Microbial community dynamics associated with rhizosphere carbon flow.Applied Environmental Microbiology,69:6793‐6800
Chen S,Yan H,Zhang L,Kong W,Sun Y,Zhang W,Chen Y,Deng A,2015.Cryptococcus neoformans infection and immune cell regulation in human monocytes.Cellular Physiology&Biochemistry,37(2):537‐547
Chen X,Sun XD,Bi SY,Zhang W,Yang H,LüGZ,Xin X,Sun XY,2010.Fungal diversity in rhizosphere soil of medicinal plants in Liaoning Province.Mycosystema,29(3):321‐328
Escudero N,Ferreira SR,Lopez‐Moya F,Naramjo‐Ortiz MA,Marin‐Ortiz AI,Thornton CR,Lopez‐Llorca LV,2016.Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia.Fungal Biology,120(4):572‐585
Garcia K,Doidy J,Zimmermann SD,Wipf D,Courty PE,2016.Take a trip through the plant and fungal transportome of mycorrhiza.Trends Plant Sciences,21:937‐950
Guba EE,1961.Monograph of Monochaetia and Pesatlotia USA.Harvard Univesrity Perss,Cambridge.342
Gurr G,Wratten SD,Waage J,2000.Measures of success in biological control.Kluwer Academic Publishers,Dordrecht,the Netherlands.231‐269
He Q,Chen WQ,Peng H,Deng BW,Jie XC,Wang Y,2017.Advances in the study of the activity of endophytic fungi and secondary metabolites of endangered plant Eucommia ulmoides.Jiangsu Agricultural Science,45(2):20‐23(in Chinese)
Huang LQ,Guo LP,Hu J,Shao AJ,2008.Molecular mechanism and genetic basis of geoherbs.China Journal of Chinese Materia Medica,33(20):2303‐2308(in Chinese)
Huang LQ,Guo LP,Ma CY,Gao W,Yuan QJ,2011.Top‐geoherbs of traditional Chinese medicine:common traits,quality characteristics and formation.Frontiers of Medicine,5(2):185‐194
Ikryl Z,Vancura V,1980.Root exudates of plants 4.Wheat root exudation as dependent on growth concentration gradient of the exudates and the presence of bacteria.Plant and Soil,57:69‐83
Inderji T,Westion LA,2003.Root exudates:an overview.Ecological Studies,168:235‐256
Kantarcioglu AS,Guarro J,de Hoog GS,Apaydin H,Kiraz N,Balkan II,Ozaras R,2016.A case of central nervous system infection due to Cladophialophora bantiana.Revista Iberoamericana de Micologia,33(4):237‐241
Li QF,2006.Dynamics of the microbial flora in the liriope rhizosphere and out rhizosphere during continuous cropping years.Chinese Journal of Soil Science,37(3):563‐565(in Chinese)
Lin KQ,Li Y,Zou BJ,Zhang TY,He XZ,Zhao DG,2016.Genetic diversity and phylogenetic relationship of cultivated Eucommia ulmoides Olive in Guizhou by SSR.Genomics and Applied Biology,35(6):1521‐1529(in Chinese)
Lu Y,Murase J,Watanabe A,Sugimoto A,Kimura M,2004.Linking microbial community dynamics to rhizosphere carbon flow in a wetland rice soil.FEMS Microbiology Ecology,48:179‐186
Morganj AW,Bending GD,White PJ,2005.Biological cost and benefits to plant‐microbe interactions in the rhizosphere.Journal of Experimental Botany,56(417):1729‐1739
Raaijmakers JM,Paulitz TC,Steinberg C,Alabouvette C,Mo?nne‐Loccoz Y,2009.The rhizosphere:a playground and battlefield for soilborne pathogens and beneficial microorganisms.Plant and Soil,321:341‐361
Salt GA,1977.The incidence of root‐surface fungi on naturally regenerated Picea sitchensis seedlings in Southeast Alaska.Forestry(Lond),50(2):113‐115
Song LS,Wei JG,Yun CG,Yang XH,Luo JT,Huang SD,2016.Survey of species diversity in the endophytic fungal genus Pestalotiopsis in Liangfengjiang National Forest Park,Guangxi,China.Scientia Silvae Sincae,52(7):87‐95(in Chinese)
Storbel G,Yang XS,Sears J,Kramer R,Sidhu RS,Hess WM,1996.Taxol from Pesatlotiopsis micorspora,an endophytic fungus of Taxus wallichiana.Microbiology,142:435‐440
Toledo AV,Virla E,Humber RA,Paradell SL,Lastra CCL,2006.First record of Clonostachys rosea(Ascomycota:Hypocreales)as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa(Hemiptera:Cicadellidae)in Argentina.Journal of Invertebrate Pathology,92:7‐10
Vandenkoornhuyse P,Baldauf SL,Leyval C,Straczek J,Young JP,2002.Extensive fungal diversity in plant roots.Science,295:2051
Wang DL,2015.Laboratory testing manual for medical fungi.People’s Medical Publishing House,Beijing.1‐525(in Chinese)
Wang RH,Zhang QF,Zhou Bl,Lian H,Ma GS,2007.Analysis on the interaction between root exudates and rhizosphere microbes.Chinese Journal of Soil Science,38(1):167‐172(in Chinese)
Whipps JM,2001.Microbial interactions and biocontrol in the rhizosphere.Journal of Experimental Botany,52:487‐511
Wu WX,Liu W,Lu HH,Chen YX,Devare M,Thies J,2009.Use of 13C labeling to assess carbon partitioning in transgenic and nontransgenic(parental)rice and their rhizosphere soil microbial communities.FEMS Microbiology Ecology,67:93‐102
Yu WH,Liu Y,Yang JJ,Wang SX,Wang XJ,2017.Screening for endophytes with PDG production from Eucommia ulmoides Oliv.Journal of Qufu Normal University,43(3):69‐76(in Chinese)
Zhang L,Sang MM,Guo XW,Xue LW,Yuang B,Cao XY,2017.Diversity of endophytic fungi isolated from Eucommia ulmoides and their antifungal activity against phytopathogenic fungi.Biotic Resources,39(1):48‐52(in Chinese)
Zhang R,Liu ZH,Yang H,Bai XM,Xu HY,2008.Analysis of ecological characteristics and classification of fungal community in rhizosphere soil of ginseng in Jilin province.Journal of Jilin Agricultural Sciences,33(1):47‐50(in Chinese)
Zheng Y,Liu W,Huang JX,Hou YR,2016.Rhizosphere microorganism evidence on geo‐authentic features of traditional Chinese medicine from Moutan(Paeonia suffruticosa).Acta Pharmaceutica Sinica,51(8):1325‐1333(in Chinese)
李琼芳,2006.不同连作年限麦冬根际微生物区系动态研究.土壤通报,37(3):563‐565
王茹华,张启发,周宝利,廉华,马光恕,2007.浅析植物根分泌物与根际微生物的相互作用关系.土壤通报,38(1):167‐172
宋利沙,韦继光,云朝光,杨秀好,罗基同,黄松殿,2016.广西良凤江国家森林公园植物内生拟盘多毛孢属真菌种类多样性调查.林业科学,52(7):87‐95
王端礼,2015.医用真菌学实验室检验手册.北京:人民卫生出版社.1‐525
黄璐琦,郭兰萍,胡娟,邵爱娟,2008.道地药材形成的分子机制及其遗传基础.中国中药杂志,33(20):2303‐2308
郑艳,刘炜,黄军祥,侯宇荣,2016.基于牡丹根际土壤微生物的中药材道地性研究.药学学报,51(8):1325‐1333
林开勤,李岩,邹冰杰,张天缘,何选泽,赵德刚,2016.贵州杜仲栽培群体遗传多样性及亲缘关系的SSR分析.基因组学与应用生物学,35(6):1521‐1529
于维虹,刘艳,杨君杰,王守霞,王雪君,2017.产松脂醇二葡萄糖苷杜仲内生菌的筛选与培养条件优化.曲阜师范大学学报,43(3):69‐76
张亮,桑曼曼,郭小伟,薛羚伟,袁博,曹小迎,2017.杜仲内生真菌的多样性分析及其抗植物病原真菌的活性.生物资源,39(1):48‐52
何强,陈文强,彭浩,邓百万,解修超,王颖,2017.濒危植物杜仲内生真菌及次生代谢产物活性研究进展.江苏农业科学,45(2):20‐23
Chen S,Yan H,Zhang L,Kong W,Sun Y,Zhang W,Chen Y,Deng A,2015.Cryptococcus neoformans infection and immune cell regulation in human monocytes.Cellular Physiology&Biochemistry,37(2):537‐547
Chen X,Sun XD,Bi SY,Zhang W,Yang H,LüGZ,Xin X,Sun XY,2010.Fungal diversity in rhizosphere soil of medicinal plants in Liaoning Province.Mycosystema,29(3):321‐328
Escudero N,Ferreira SR,Lopez‐Moya F,Naramjo‐Ortiz MA,Marin‐Ortiz AI,Thornton CR,Lopez‐Llorca LV,2016.Chitosan enhances parasitism of Meloidogyne javanica eggs by the nematophagous fungus Pochonia chlamydosporia.Fungal Biology,120(4):572‐585
Garcia K,Doidy J,Zimmermann SD,Wipf D,Courty PE,2016.Take a trip through the plant and fungal transportome of mycorrhiza.Trends Plant Sciences,21:937‐950
Guba EE,1961.Monograph of Monochaetia and Pesatlotia USA.Harvard Univesrity Perss,Cambridge.342
Gurr G,Wratten SD,Waage J,2000.Measures of success in biological control.Kluwer Academic Publishers,Dordrecht,the Netherlands.231‐269
He Q,Chen WQ,Peng H,Deng BW,Jie XC,Wang Y,2017.Advances in the study of the activity of endophytic fungi and secondary metabolites of endangered plant Eucommia ulmoides.Jiangsu Agricultural Science,45(2):20‐23(in Chinese)
Huang LQ,Guo LP,Hu J,Shao AJ,2008.Molecular mechanism and genetic basis of geoherbs.China Journal of Chinese Materia Medica,33(20):2303‐2308(in Chinese)
Huang LQ,Guo LP,Ma CY,Gao W,Yuan QJ,2011.Top‐geoherbs of traditional Chinese medicine:common traits,quality characteristics and formation.Frontiers of Medicine,5(2):185‐194
Ikryl Z,Vancura V,1980.Root exudates of plants 4.Wheat root exudation as dependent on growth concentration gradient of the exudates and the presence of bacteria.Plant and Soil,57:69‐83
Inderji T,Westion LA,2003.Root exudates:an overview.Ecological Studies,168:235‐256
Kantarcioglu AS,Guarro J,de Hoog GS,Apaydin H,Kiraz N,Balkan II,Ozaras R,2016.A case of central nervous system infection due to Cladophialophora bantiana.Revista Iberoamericana de Micologia,33(4):237‐241
Li QF,2006.Dynamics of the microbial flora in the liriope rhizosphere and out rhizosphere during continuous cropping years.Chinese Journal of Soil Science,37(3):563‐565(in Chinese)
Lin KQ,Li Y,Zou BJ,Zhang TY,He XZ,Zhao DG,2016.Genetic diversity and phylogenetic relationship of cultivated Eucommia ulmoides Olive in Guizhou by SSR.Genomics and Applied Biology,35(6):1521‐1529(in Chinese)
Lu Y,Murase J,Watanabe A,Sugimoto A,Kimura M,2004.Linking microbial community dynamics to rhizosphere carbon flow in a wetland rice soil.FEMS Microbiology Ecology,48:179‐186
Morganj AW,Bending GD,White PJ,2005.Biological cost and benefits to plant‐microbe interactions in the rhizosphere.Journal of Experimental Botany,56(417):1729‐1739
Raaijmakers JM,Paulitz TC,Steinberg C,Alabouvette C,Mo?nne‐Loccoz Y,2009.The rhizosphere:a playground and battlefield for soilborne pathogens and beneficial microorganisms.Plant and Soil,321:341‐361
Salt GA,1977.The incidence of root‐surface fungi on naturally regenerated Picea sitchensis seedlings in Southeast Alaska.Forestry(Lond),50(2):113‐115
Song LS,Wei JG,Yun CG,Yang XH,Luo JT,Huang SD,2016.Survey of species diversity in the endophytic fungal genus Pestalotiopsis in Liangfengjiang National Forest Park,Guangxi,China.Scientia Silvae Sincae,52(7):87‐95(in Chinese)
Storbel G,Yang XS,Sears J,Kramer R,Sidhu RS,Hess WM,1996.Taxol from Pesatlotiopsis micorspora,an endophytic fungus of Taxus wallichiana.Microbiology,142:435‐440
Toledo AV,Virla E,Humber RA,Paradell SL,Lastra CCL,2006.First record of Clonostachys rosea(Ascomycota:Hypocreales)as an entomopathogenic fungus of Oncometopia tucumana and Sonesimia grossa(Hemiptera:Cicadellidae)in Argentina.Journal of Invertebrate Pathology,92:7‐10
Vandenkoornhuyse P,Baldauf SL,Leyval C,Straczek J,Young JP,2002.Extensive fungal diversity in plant roots.Science,295:2051
Wang DL,2015.Laboratory testing manual for medical fungi.People’s Medical Publishing House,Beijing.1‐525(in Chinese)
Wang RH,Zhang QF,Zhou Bl,Lian H,Ma GS,2007.Analysis on the interaction between root exudates and rhizosphere microbes.Chinese Journal of Soil Science,38(1):167‐172(in Chinese)
Whipps JM,2001.Microbial interactions and biocontrol in the rhizosphere.Journal of Experimental Botany,52:487‐511
Wu WX,Liu W,Lu HH,Chen YX,Devare M,Thies J,2009.Use of 13C labeling to assess carbon partitioning in transgenic and nontransgenic(parental)rice and their rhizosphere soil microbial communities.FEMS Microbiology Ecology,67:93‐102
Yu WH,Liu Y,Yang JJ,Wang SX,Wang XJ,2017.Screening for endophytes with PDG production from Eucommia ulmoides Oliv.Journal of Qufu Normal University,43(3):69‐76(in Chinese)
Zhang L,Sang MM,Guo XW,Xue LW,Yuang B,Cao XY,2017.Diversity of endophytic fungi isolated from Eucommia ulmoides and their antifungal activity against phytopathogenic fungi.Biotic Resources,39(1):48‐52(in Chinese)
Zhang R,Liu ZH,Yang H,Bai XM,Xu HY,2008.Analysis of ecological characteristics and classification of fungal community in rhizosphere soil of ginseng in Jilin province.Journal of Jilin Agricultural Sciences,33(1):47‐50(in Chinese)
Zheng Y,Liu W,Huang JX,Hou YR,2016.Rhizosphere microorganism evidence on geo‐authentic features of traditional Chinese medicine from Moutan(Paeonia suffruticosa).Acta Pharmaceutica Sinica,51(8):1325‐1333(in Chinese)
李琼芳,2006.不同连作年限麦冬根际微生物区系动态研究.土壤通报,37(3):563‐565
王茹华,张启发,周宝利,廉华,马光恕,2007.浅析植物根分泌物与根际微生物的相互作用关系.土壤通报,38(1):167‐172
宋利沙,韦继光,云朝光,杨秀好,罗基同,黄松殿,2016.广西良凤江国家森林公园植物内生拟盘多毛孢属真菌种类多样性调查.林业科学,52(7):87‐95
王端礼,2015.医用真菌学实验室检验手册.北京:人民卫生出版社.1‐525
黄璐琦,郭兰萍,胡娟,邵爱娟,2008.道地药材形成的分子机制及其遗传基础.中国中药杂志,33(20):2303‐2308
郑艳,刘炜,黄军祥,侯宇荣,2016.基于牡丹根际土壤微生物的中药材道地性研究.药学学报,51(8):1325‐1333
林开勤,李岩,邹冰杰,张天缘,何选泽,赵德刚,2016.贵州杜仲栽培群体遗传多样性及亲缘关系的SSR分析.基因组学与应用生物学,35(6):1521‐1529
于维虹,刘艳,杨君杰,王守霞,王雪君,2017.产松脂醇二葡萄糖苷杜仲内生菌的筛选与培养条件优化.曲阜师范大学学报,43(3):69‐76
张亮,桑曼曼,郭小伟,薛羚伟,袁博,曹小迎,2017.杜仲内生真菌的多样性分析及其抗植物病原真菌的活性.生物资源,39(1):48‐52
何强,陈文强,彭浩,邓百万,解修超,王颖,2017.濒危植物杜仲内生真菌及次生代谢产物活性研究进展.江苏农业科学,45(2):20‐23