巴氏生孢八叠球菌用作混凝土裂缝愈合剂的活性研究
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摘要
针对巴氏生孢八叠球菌作为生物愈合剂用于混凝土修复可能面临的复杂环境,开展了强碱耐受性、尿素浓度适应性、温度适应性等试验,考察了不同环境下的细菌活性与碳酸钙产量.结果表明:混凝土内部的强碱环境逼近或可能超出巴氏生孢八叠球菌的强碱耐受极限;细菌活性随尿素浓度与温度升高而升高,推荐愈合剂尿素浓度为0.6mol/L,建议夏季施工.细菌-尿素-醋酸钙愈合剂体系产物为球霰石,该体系可用于混凝土裂缝修补.
When Sporosarcina pasteurii serves as bio-healing agent of concrete,it is inevitably exposed to a complex environment.In order to study the bacteria activity and calcium carbonate production of Sporosarcina pasteurii in different conditions,experiments such as strong-alkaline tolerance,urea concentration adaptability and temperature adaptability were conducted.Results show that the strong-alkaline environment in concrete is close to,or even higher than,the limit of alkaline tolerance of the bacteria.Moreover,bacteria activity would be enhanced as urea concentration and temperature increase.It is recommended that the urea concentration in healing agent should be 0.6 mol/L.Additionally,optimal application time of the healing agent is in summer.Results prove that the production of Sporosarcina pasteurii-urea-calcium acetate system is vaterite.This system can be used for concrete crack healing.
When Sporosarcina pasteurii serves as bio-healing agent of concrete,it is inevitably exposed to a complex environment.In order to study the bacteria activity and calcium carbonate production of Sporosarcina pasteurii in different conditions,experiments such as strong-alkaline tolerance,urea concentration adaptability and temperature adaptability were conducted.Results show that the strong-alkaline environment in concrete is close to,or even higher than,the limit of alkaline tolerance of the bacteria.Moreover,bacteria activity would be enhanced as urea concentration and temperature increase.It is recommended that the urea concentration in healing agent should be 0.6 mol/L.Additionally,optimal application time of the healing agent is in summer.Results prove that the production of Sporosarcina pasteurii-urea-calcium acetate system is vaterite.This system can be used for concrete crack healing.
引文
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[3]许朝阳,张莉.微生物改性对粉土强度的影响[J].建筑科学,2009,25(5):45-48.XU Zhaoyang,ZHANG Li.The effect of microbes on strength of silt[J].Building Science,2009,25(5):45-48(in Chinese)
[4]HE Jia,CHU Jian,WU Shifan,et al.Mitigation of soil liquefaction using microbially induced desaturation[J].Journal of Zhejiang University,SCIENCE A(Applied Physics&Engineering),2016,17(7):577-588.
[5]WIKTOR V,JONKERS H M.Quantification of crack-healing in novel bacteria-based self-healing concrete[J].Cement and Concrete Composite,2011,33(7):763-770.
[6]VAN TITTELBOOM K,DE BELIE N,DE MUYNCK W,et al.Use of bacteria to repair cracks in concrete[J].Cement and Concrete Research,2011,40(1):157-166.
[7]李沛豪,屈文俊.细菌诱导碳酸钙沉积修复混凝土裂缝[J].土木工程学报,2010,43(11):64-70.LI Peihao,QU Wenjun.Remediation of concrete cracks by bacterially-induced calcium carbonate deposition[J].China Civil Engineering Journal,2010,43(11):64-70.(in Chinese)
[8]钱春香,李瑞阳,潘庆峰,等.混凝土裂缝的微生物自修复效果[J].东南大学学报(自然科学版),2013,43(2):360-364.QIAN Chunxiang,LI Ruiyang,PAN Qingfeng,et al.Microbial self-healing effects of concrete cracks[J].Journal of Southeast University(Natural Science Edition),2013,43(2):360-364.(in Chinese)
[9]BANG S S,GALINAT J K,RAMAKRISHNAN V.Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii[J].Enzyme and Microbial Technology,2001,28(4):404-409.
[10]OKWADHA G D O,LI J.Optimum conditions for microbial carbonate precipitation[J].Chemosphere,2010,81(9):1143-1148.
[11]赵茜.微生物诱导碳酸钙沉淀(MICP)固化土壤实验研究[D].北京:中国地质大学,2014.ZHAO Qian.Experimental study on soil improvement using microbial induced calcite precipitation(MICP)[D].Beijing:China University of Geosciences,2014.(in Chinese)
[12]李沛豪,屈文俊,徐德强,等.大理石历史建筑遗产的细菌修复加固[J].华南理工大学学报(自然科学版),2009,37(9):36-41.LI Peihao,QU Wenjun,XU Deqiang,et al.Remediation of historic marble architectural heritages by bacterially-induced biomineralization[J].Journal of South China University of Technology(Natural Science Edition),2009,37(9):36-41.(in Chinese)
[13]贾强,赵程程,孙增斌.微生物沉积碳酸钙修复混凝土裂缝抗渗性研究[J].应用基础与工程科学学报,2017,25(1):141-148.JIA Qiang,ZHAO Chengcheng,SUN Zengbin.Bearing pressure experimental research of the concrete cracks after bioremedying[J].Journal of Basic Science and Engineering,2017,25(1):141-148.(in Chinese)
[14]MOYO C C,KISSEL D E,GABRERA M L.Temperature effects on soil urease activity[J].Soil Biology and Biochemistry,1989,21(7):935-938.