鹌鹑IGF-I、MyoD基因多态性与其肉质性状的关联分析
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摘要
为探讨不同基因型与肉质性状的相关性,为鹌鹑的分子标记辅助选择积累理论资料,选择150只8周龄的优质朝鲜鹌鹑为试验对象,采用PCR-SSCP技术检测基因多态性,分析不同基因型与肉质性状的相关性。结果显示:IGF-I(胰岛素1号增长因子)基因检测到多态性,发现了AA、GG两种不同的基因型,在A36127G处存在碱基突变,在肌内脂肪含量上AA与GG基因型之间差异显著(P<0.05);MyoD(成肌分化抗原)基因也具有多态性,发现了CC、CT、TT三种基因型,在T22453C处存在碱基突变,在肌内脂肪含量上CT与TT基因型之间差异显著(P<0.05)。结果表明,IGF-I、MyoD基因可以作为鹌鹑肉质性状辅助选择分子标记的候选基因。
In order to explore the correlation between different genotypes and their meat quality traits, theoretical data were accumulated for the molecular marker-assisted selection of sputum. In this study, 150 8-week-age high-quality artichokes were selected as test subjects. PCR-SSCP technique was used to detect gene polymorphisms, and the correlation between different genotypes and meat quality traits was analyzed. The results showed that the polymorphism was successfully detected in IGF-I gene, and two different genotypes of AA and GG were found. There were base mutations at A36127 G,and the difference between AA and GG genotypes was significant in intramuscular fat(P<0.05). MyoD gene also has polymorphism. Three genotypes of CC, CT and TT were found. Sequencing revealed that there were base mutations at T22453 C, and CT and TT genotypes in intramuscular fat expression. The difference was significant(P<0.05). Mutations in bases result in polymorphisms in genes, and different expression levels of different genotypes further affect the expression of meaty traits. The results indicated that IGF-I and MyoD genes could be candidate genes for molecular markers for selective selection of succulent meat traits.
In order to explore the correlation between different genotypes and their meat quality traits, theoretical data were accumulated for the molecular marker-assisted selection of sputum. In this study, 150 8-week-age high-quality artichokes were selected as test subjects. PCR-SSCP technique was used to detect gene polymorphisms, and the correlation between different genotypes and meat quality traits was analyzed. The results showed that the polymorphism was successfully detected in IGF-I gene, and two different genotypes of AA and GG were found. There were base mutations at A36127 G,and the difference between AA and GG genotypes was significant in intramuscular fat(P<0.05). MyoD gene also has polymorphism. Three genotypes of CC, CT and TT were found. Sequencing revealed that there were base mutations at T22453 C, and CT and TT genotypes in intramuscular fat expression. The difference was significant(P<0.05). Mutations in bases result in polymorphisms in genes, and different expression levels of different genotypes further affect the expression of meaty traits. The results indicated that IGF-I and MyoD genes could be candidate genes for molecular markers for selective selection of succulent meat traits.
引文
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[2]ZAMMIT P S.Function of the myogenic regulatory factors Myf5,MyoD,Myogenin and Mrf4 in skeletal muscle,satellite cells and regenerative myogenesis[J].Seminars in Cell and Developmental Biology,2017,72:19-32.
[3]范红英,陈圆,曾凤群,等.不同日龄惠阳胡须鸡肌肉中IGF1、MyoD基因表达研究[J].惠州学院学报,2017,37(3):61-64.
[4]王强.鸡IGF-Ⅰ基因PCR-SSCP分析及其与肉用性状关系的研究[D].扬州:扬州大学,2006.
[5]魏笑笑,王宝维,王雷,等.琅琊鸡IGF-Ⅰ基因多态性与肉用性的相关性[J].福建农林大学学报(自然科学版),2008,37(4):389-393.
[6]徐晶.吉林白鹅IGFⅡ基因SNP及其与肉用性状相关性的研究[D].长春:吉林农业大学,2007.
[7]赵秀华,王金玉,张跟喜,等.IGF-I与IGFBP-1基因对京海黄鸡生长性状的遗传效应分析[J].畜牧兽医学报,2012,43(1):152-158.
[8]王琼.MyoD、MyoG基因的多态性及其与鸡屠宰性状和肉质性状的相关性研究[D].雅安:四川农业大学,2007.
[9]COMMITTEE N R N.A Unified Nomenclature System for the Nuclear Receptor Superfamily[J].Cell,1999,97(2):161-163.
[10]赵广珍,贾青,张翠翠,等.猪MyoD1基因多态性与肉质性状的相关分析[J].河北农业大学学报,2012,35(4):90-94,99.
[11]URBANSKI P,KURY?J.New SNPs in the coding and5′flanking regions of porcine MYOD1(MYF3)and MYF5genes[J].Journal of Applied Genetics,2004,45(3):325-329.