摘要
【目的】对大豆品种北农103中的矮秆性状进行遗传分析和初步基因定位,为该基因的精细定位和育种利用奠定基础。【方法】以高秆大豆品种海系13和矮秆品种北农103为研究材料,通过对(海系13×北农103)F4的剩余杂合体(RHL)F2分离群体的遗传分析,解析控制北农103矮秆性状基因的遗传规律,同时以(海系13×北农103)的(RHL)F2∶3为作图群体,利用BSA法结合SSR分子标记,对北农103中的矮秆基因进行分子标记和遗传定位。【结果】北农103中携带1对控制矮秆性状的隐性基因GmD1;经分子标记连锁分析,该基因与9个SSR标记连锁,分别是Satt527、Satt166、Sat_340、Satt481、Sat_150、Sat_245、Satt076、Satt229和Satt664。【结论】根据大豆遗传图谱推断,该基因位于大豆L连锁群(19染色体)上,在satt527和satt166标记之间,遗传距离分别是3.7cM和4.2cM,推测为一个新的控制大豆矮秆的基因GmD1,这些发现和分子标记定位对于该基因的精细定位将起到重要作用。
【Objective】The dwarf soybean variety BeiNong 103 was researched by genetic analysis and preliminary gene localization for fine mapping and breeding utilization.【Methods】To genetic analysis and gene mapping,a F2:4 segregating population derived from a cross of high-stalk cultivar HaiXi 13 with a dwarf-stalk accession BeiNong 103.And the BSA methods was used as SSR primers screening and gene localization.【Results】Genetic analysis showed that the dwarf trait of BeiNong 103 was controlled by a recessive gene GmD1.Satt527,Satt166,Sat_340,Satt481,Sat_150,Sat_245,Satt076,Satt229 and Satt664,9 SSR markers were found linkaged with the GmD1 gene respectively.【Conclusion】According to the gene location,the GmD1 gene was located on the soybean L-linked group(chromosome 19),and the genetic distance were 3.7 cM 和4.2 cM between the satt527 and satt166.The speculate that GmD1 was a new dwarf gene of soybean.The results provide important information for the fine mapping of genes.
引文
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