摘要
目的采用闪式提取法提取金锦香总黄酮并考察其体外抗氧化活性。方法通过Box-Behnken中心组合设计,以乙醇体积分数、料液比、提取时间和提取电压为自变量,金锦香总黄酮得率为因变量,优化提取工艺。同时还考察了金锦香总黄酮对DPPH自由基和·OH自由基的清除作用。结果闪式提取金锦香总黄酮最佳工艺条件为:乙醇体积分数65%、料液比1∶30(g/mL)、提取时间60 s、提取电压60 V,金锦香总黄酮得率为(43.46±0.07)%。金锦香总黄酮对DPPH及·OH自由基具有明显的清除能力,最大清除率分别达到91.06%、88.98%。结论该提取方法高效、快速、经济,而且金锦香总黄酮具有较好的抗氧化作用,为金锦香的综合开发利用提供了理论基础。
Objective To extract the total flavonoids of Osbeckia chinensis Linn. ex Walp by flash extraction and determine its antioxidant activity in vitro. Methods In the Box-Behnken center combination design, the ethanol volume fraction, solid-liquid ratio, extraction time and extraction voltage were independent variables.The total flavonoids yield of Osbeckia chinensis Linn. ex Walp was dependent variable in the optimized extraction process. At the same time, the scavenging effects of total flavonoids on DPPH and ·OH radicals were also determined. Results The optimum conditions for the extraction of total flavonoids of Osbeckia chinensis Linn. ex Walp were as follows: ethanol volume fraction at 65%, solid-liquid ratio at 1 ∶ 30(g/mL), extraction time at 60 s, extraction voltage at 60 V, and total flavonoids yield was(43.46±0.07)%. The total flavonoids had obvious scavenging effect on DPPH and ·OH radicals, and the maximum clearance rates were 91.06%and 88.98%, respectively. Conclusion The extraction method is rapid, efficient and economical, and the total flavonoids of Osbeckia chinensis Linn. ex Walp have a good antioxidant effect, which provides a theoretical basis for the comprehensive development and utilization of Osbeckia chinensis Linn. ex Walp.
引文
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