基于Box-Behnken模型优化金锦香总黄酮闪式提取工艺及其抗氧化活性分析
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摘要
目的采用闪式提取法提取金锦香总黄酮并考察其体外抗氧化活性。方法通过Box-Behnken中心组合设计,以乙醇体积分数、料液比、提取时间和提取电压为自变量,金锦香总黄酮得率为因变量,优化提取工艺。同时还考察了金锦香总黄酮对DPPH自由基和·OH自由基的清除作用。结果闪式提取金锦香总黄酮最佳工艺条件为:乙醇体积分数65%、料液比1∶30(g/mL)、提取时间60 s、提取电压60 V,金锦香总黄酮得率为(43.46±0.07)%。金锦香总黄酮对DPPH及·OH自由基具有明显的清除能力,最大清除率分别达到91.06%、88.98%。结论该提取方法高效、快速、经济,而且金锦香总黄酮具有较好的抗氧化作用,为金锦香的综合开发利用提供了理论基础。
Objective To extract the total flavonoids of Osbeckia chinensis Linn. ex Walp by flash extraction and determine its antioxidant activity in vitro. Methods In the Box-Behnken center combination design, the ethanol volume fraction, solid-liquid ratio, extraction time and extraction voltage were independent variables.The total flavonoids yield of Osbeckia chinensis Linn. ex Walp was dependent variable in the optimized extraction process. At the same time, the scavenging effects of total flavonoids on DPPH and ·OH radicals were also determined. Results The optimum conditions for the extraction of total flavonoids of Osbeckia chinensis Linn. ex Walp were as follows: ethanol volume fraction at 65%, solid-liquid ratio at 1 ∶ 30(g/mL), extraction time at 60 s, extraction voltage at 60 V, and total flavonoids yield was(43.46±0.07)%. The total flavonoids had obvious scavenging effect on DPPH and ·OH radicals, and the maximum clearance rates were 91.06%and 88.98%, respectively. Conclusion The extraction method is rapid, efficient and economical, and the total flavonoids of Osbeckia chinensis Linn. ex Walp have a good antioxidant effect, which provides a theoretical basis for the comprehensive development and utilization of Osbeckia chinensis Linn. ex Walp.
Objective To extract the total flavonoids of Osbeckia chinensis Linn. ex Walp by flash extraction and determine its antioxidant activity in vitro. Methods In the Box-Behnken center combination design, the ethanol volume fraction, solid-liquid ratio, extraction time and extraction voltage were independent variables.The total flavonoids yield of Osbeckia chinensis Linn. ex Walp was dependent variable in the optimized extraction process. At the same time, the scavenging effects of total flavonoids on DPPH and ·OH radicals were also determined. Results The optimum conditions for the extraction of total flavonoids of Osbeckia chinensis Linn. ex Walp were as follows: ethanol volume fraction at 65%, solid-liquid ratio at 1 ∶ 30(g/mL), extraction time at 60 s, extraction voltage at 60 V, and total flavonoids yield was(43.46±0.07)%. The total flavonoids had obvious scavenging effect on DPPH and ·OH radicals, and the maximum clearance rates were 91.06%and 88.98%, respectively. Conclusion The extraction method is rapid, efficient and economical, and the total flavonoids of Osbeckia chinensis Linn. ex Walp have a good antioxidant effect, which provides a theoretical basis for the comprehensive development and utilization of Osbeckia chinensis Linn. ex Walp.
引文
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[3]刘杰,陈海生,张卫东.金锦香属植物的研究概况[J].解放军药学学报,2001,17(3):151-153.
[4]陈冠,崔承彬,蔡兵,等.野牡丹科十属植物研究进展[J].天然产物研究与开发,2006,18:863-872.
[5]赵友兴,杨丹,马青云,等.金锦香的化学成分研究[J].中草药,2011,42(6):1061-1065.
[6]赵雪巍,刘培玉,刘丹,等.黄酮类化合物的构效关系研究进展[J].中草药,2015,46(21):3264-3271.
[7]徐瑞超,董自亮.响应面法优化大豆异黄酮闪式提取工艺研究[J].成都中医药大学学报,2012,35(1):48-51.
[8]Tao Tao,He Fan,Ji Xiao-ming. Response surface methodology for optimization of flash extraction for fenugreek(Trigonella foenum-graecum L.)polysaccharides and research of its humectant properties[J]. Fine Chemicals,2016,33(6):666-673.
[9]张英,吴晓琴,丁霄霖.黄酮类化合物结构与清除活性氧自由基效能关系的研究[J].天然产物研究与开发,1998,10(4):26-33.
[10]饶建平,王文成,李国辉,等.响应面法优化咖啡生豆水提绿原酸工艺研究[J].食品工业科技,2015,36(22):298-301,319.
[11]汤磊,刘本.响应面法优化白芍中芍药苷的超临界流体提取研究[J].中成药,2010,32(8):1332-1337.
[12]王文成,林衍生,连建枝,等.金线莲根茎水提工艺优化研究[J].江苏农业科学,2015,43(7):287-290.
[13]赵科,解红霞,张娜.响应面法优化黄花铁线莲总皂苷超声提取工艺[J].中华中医药杂志,2015,30(4):1239-1241.
[14]Silva EM,Souza JNS,Rogez H,et al. Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region[J]. Food Chem,2007,101(3):1012-1018.
[15]ProcházkováD,Bou?ováI,WilhelmováN. Antioxidant and prooxidant properties of flavonoids[J]. Fitoterapia,2011,82(4):513-523.
[16]赵伯涛,钱骅,张卫明.抗氧化植物资源的研究与开发[J].中国野生植物资源,1998,17(3):18-20.
[17]杨红叶,杨联芝,柴岩,等.甜荞和苦荞籽中多酚存在形式与抗氧化活性的研究[J].食品工业科技,2011,32(5):90-94,97.
[18]Zdunczyk Z,Frejnagel S,Wro blewska M,et al. Biological activity of polyphenol extracts from different plant sources[J]. Food Res Int,2002,35(2):183-186.