摘要
针对纳米缺钙羟基磷灰石/壳聚糖(nCDHA/CS)复合微球中,nCDHA在微球中分布不均和含量不足的问题,在油包水(W/O)体系中,运用均匀沉淀法原位制备了nCDHA/CS复合微球。利用扫描电镜(SEM)、粒度分析仪、X射线衍射(XRD)、傅里叶红外光谱仪(FT-IR)、热重分析(TG)、X射线光电子能谱仪(XPS)等对复合微球的理化性能进行了表征。结果显示,所制得的nCDHA/CS复合微球中,nCDHA均匀分布于复合微球中,其含量高达43%;复合微球粒径分布较窄,球形度良好,分散性指数(PDI)为0.291,平均粒径18.6μm。仿生矿化结果显示,复合微球表面矿化是从nCDHA生成nHA的过程,仿生矿化14 d后,微球表面形成大量均匀的片状类骨磷灰石,表明该复合微球具有较好的生物学性能,对骨组织再生修复具有较大的潜力。
Aimed at the problem of nano calcium-deficient hydroxyapatite(nCDHA) uneven distribution and insufficient content in nano calcium-deficient hydroxyapatite/chitosan(CS) composite microspheres, nCDHA/CS composite microspheres were firstly fabricated in situ by homogeneous precipitation method in a water-in-oil(W/O) emulsion. The physicochemical properties of composite microspheres were investigated by scanning electronic microscopy(SEM), laser particle size analyzer, X-ray diffraction(XRD), Fourier infrared spectrum(FT-IR), thermogravimetric analysis(TG) and X-ray photoelectron spectroscopy(XPS). The results indicate that the nCDHA was uniformly distributed in the nCDHA/CS composite microspheres, and the content was up to 43%. Moreover, the composite microspheres had a narrow size distribution, good sphericity with PDI 0.291 and average size of 18.6 μm. The result of biomimetic mineralization shows that the surface mineralization of composite microspheres was a process from nCDHA to nHA. After 14 d of biomimetic mineralizationr, a large number of even sheets of bone-like apatite were formed on the surface of the microspheres, indicating that the composite microspheres had good biological properties and great potential for bone tissue regeneration and repair.
引文
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