淫羊藿和甘草配伍对淫羊藿主要化学成分的影响
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摘要
目的研究淫羊藿与甘草不同比例配伍时淫羊藿主要化学成分的影响。方法将淫羊藿与甘草分别按照不同比例配伍,水煎,制备供试样品,采用HPLC法测定淫羊藿苷的含量,Kromasil C_(18)(250 mm×4.6 mm,5μm)型色谱柱,以水为流动相A,乙腈为流动相B,在流速为1.0 mL·min~(-1)下进行洗脱;检测波长:270 nm;柱温:25℃。结果淫羊藿与甘草配伍后,各配伍组淫羊藿中淫羊藿苷等多种成分的含量均有不同程度的增加,在淫羊藿与甘草的配伍比例为5∶1时含量增加最明显,有些成分含量则无明显改变。结论淫羊藿与甘草配伍后药材中淫羊藿苷等成分溶出量较单味淫羊藿提高,可为二药配伍应用提供参考。
Objective To study the changes of the main chemical constituents in decocting Epimedium brevicornu Maxim and Glycyrrhiza separately and compatibly.Methods Epimedium brevicornu Maxim and Glycyrrhiza were mixed with each other in different proportions,and decocted with water.The samples were prepared and the content of icariin was determined by HPLC.Kromasil C_(18)(250 mm×4.6 mm,5 μm) was used to determine the content of icariin with water as mobile phase A and acetonitrile as mobile phase B.The elution was carried out at 1.0 mL·min~(-1).The detection wavelength was 270 nm and the column temperature was 25 ℃.Results After combined decoction of Epimedium brevicornu Maxim and Glycyrrhiza,the contents of some chemical constituents such as icariin in Epimedium brevicornu Maxim were increased,especially in the compatible decoction at the proportion 5∶1.Some other constituents were not changed obviously.Conclusion The decoction of Epimedium brevicornu Maxim and Glycyrrhiza can increase the solubility of the chemical constituents in Epimedium brevicornu Maxim.This provides a scientific basis for more effective use of the compatible decoction of the 2 herbs.
Objective To study the changes of the main chemical constituents in decocting Epimedium brevicornu Maxim and Glycyrrhiza separately and compatibly.Methods Epimedium brevicornu Maxim and Glycyrrhiza were mixed with each other in different proportions,and decocted with water.The samples were prepared and the content of icariin was determined by HPLC.Kromasil C_(18)(250 mm×4.6 mm,5 μm) was used to determine the content of icariin with water as mobile phase A and acetonitrile as mobile phase B.The elution was carried out at 1.0 mL·min~(-1).The detection wavelength was 270 nm and the column temperature was 25 ℃.Results After combined decoction of Epimedium brevicornu Maxim and Glycyrrhiza,the contents of some chemical constituents such as icariin in Epimedium brevicornu Maxim were increased,especially in the compatible decoction at the proportion 5∶1.Some other constituents were not changed obviously.Conclusion The decoction of Epimedium brevicornu Maxim and Glycyrrhiza can increase the solubility of the chemical constituents in Epimedium brevicornu Maxim.This provides a scientific basis for more effective use of the compatible decoction of the 2 herbs.
引文
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[14] 王钧.高效液相色谱法测定健阳片中淫羊藿苷和甘草酸的含量[J].内蒙古中医药,2017,36(9):142-143.
[15] 罗毅,杨俊,张倩睿,等.配伍黄芪对淫羊藿主要化学成分的影响[J].中国中医药信息杂志,2016,13(12):46-48.
[16] 路宇仁,陈昳冰,崔元璐,等.淫羊藿苷药理作用研究进展[J].中国实验方剂学杂志,2018,24(17):209-220.
[17] 李钊,王雄彪.淫羊藿甙的研究进展[J].现代中西医结合杂志,2015,24(8):908-910.
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[19] 吕邵娃,蔡书茵,郭玉岩,等.甘草皂苷对难溶性化合物茯苓酸大鼠体内吸收的影响[J].中医药导报,2012,40(5):52-54.
[2] 雷美娜,肖会敏,何悦,等.乐安眠茶水溶性成分的HPLC指纹图谱及2种成分的溶出度研究[J].西北药学杂志,2018,33(3):318-323.
[3] 孟宁,孔凯,李师翁.淫羊藿属植物化学成分及药理活性研究进展[J].西北植物学报,2010,30(5):1063-1073.
[4] 赵燕燕,徐本明,姜斌,等.淫羊藿总黄酮提取工艺对总黄酮和淫羊藿苷含量的影响[J].烟台大学学报:自然科学与工程版,2007,20(1):22-25.
[5] 王兵,王亚新,赵红燕,等.甘草的主要成分及其药理作用的研究进展[J].吉林医药学院学报,2013,34(3):215-218.
[6] 杨璇.甘草对葛根、黄芩增溶作用和机理的研究[D].北京:北京中医药大学,2013.
[7] 鲁冰,杨璇,王宝华,等.甘草酸对黄芩苷增溶作用的研究[J].北京中医药大学学报,2014,37(9):620-624.
[8] 陈璐,许润春,邹文铨,等.甘草-青黛药对配伍的增溶作用考察[J].中国实验方剂学杂志,2012,18(23):17-19.
[9] 苏彬,李晓波.配伍甘草对大黄泻下作用影响的研究进展[J].中国中药杂志,2015,40(4):577-581.
[10] 雷永涛,梁妍,郝小燕,等.不同产地淫羊藿中4种活性成分含量的高效液相色谱法测定[J].时珍国医国药,2013,24(6):1404-1405.
[11] 邓晓君,曹晴,龙秀红.不同品种淫羊藿的淫羊藿苷含量研究[J].广东化工,2014,41(5):65-66.
[12] 国家药典委员会.中国药典:2015年版:一部[S].北京:中国医药科技出版社,2015.
[13] 郭青,张庆生.反相高效液相色谱法测定淫羊藿中淫羊藿苷的含量及采用其指纹色谱图鉴定淫羊藿[J].中国药事,2014,18(11):687-688.
[14] 王钧.高效液相色谱法测定健阳片中淫羊藿苷和甘草酸的含量[J].内蒙古中医药,2017,36(9):142-143.
[15] 罗毅,杨俊,张倩睿,等.配伍黄芪对淫羊藿主要化学成分的影响[J].中国中医药信息杂志,2016,13(12):46-48.
[16] 路宇仁,陈昳冰,崔元璐,等.淫羊藿苷药理作用研究进展[J].中国实验方剂学杂志,2018,24(17):209-220.
[17] 李钊,王雄彪.淫羊藿甙的研究进展[J].现代中西医结合杂志,2015,24(8):908-910.
[18] 马鸿雁,李霞,李楠,等.甘草及其活性成分的表面活性比较研究[J].现代药物与临床,2011,26(3):227-229.
[19] 吕邵娃,蔡书茵,郭玉岩,等.甘草皂苷对难溶性化合物茯苓酸大鼠体内吸收的影响[J].中医药导报,2012,40(5):52-54.