重瓣榆叶梅茎段组织培养体系的建立
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摘要
以重瓣榆叶梅带腋芽的茎段作为试验材料,研究了不同外植体消毒方式、初代培养基激素配比和生根培养基激素配比对重瓣榆叶梅组织培养的影响。结果表明:1)先用75%酒精处理30 s,再使用0.1%升汞溶液消毒8 min或以2%次氯酸钠溶液消毒12 min,外植体感染率最低;2)茎段初代培养最佳培养基为MS+6-BA2.0 mg/L+NAA 0.05 mg/L,诱导率为89.30%;3)最佳增殖培养基为MS+6-BA 2.0 mg/L+NAA 0.1 mg/L+KT1.0 mg/L,增殖倍数为4.4,且生长状况良好;4)1/2MS+IBA 0.2 mg/L为最佳生根培养基;5)该研究建立了重瓣榆叶梅组培再生体系,为工厂化育苗与大面积推广奠定了基础。
The stem segments with the axillary buds of Amygdalus triloba f. multiplex were used as test materials. The effects of different explants disinfection ways, primary culture medium hormone ratio and rooting medium hormone ratio on tissue culture of A. triloba were studied. The results show that: 1) Firstly the explant was treated with 75% alcohol for 30 s, then disinfected with 0.1% mercuric chloride solution for 8 min or disinfected with 2% sodium hypochlorite solution for 12 min, the explant infection rate was the lowest;2) The optimal medium for primary culture of stem segments was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L, and the induction rate was89.30%; 3) The optimal proliferation medium was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + KT 1.0 mg/L, the proliferation multiple was4.4, and the growth condition was good; 4) 1/2 MS + IBA 0.2 mg/L medium was the best rooting medium; 5) This study established a tissue culture regeneration system of A. triloba, which laid a foundation for planting seedlings and large-scale promotion.
The stem segments with the axillary buds of Amygdalus triloba f. multiplex were used as test materials. The effects of different explants disinfection ways, primary culture medium hormone ratio and rooting medium hormone ratio on tissue culture of A. triloba were studied. The results show that: 1) Firstly the explant was treated with 75% alcohol for 30 s, then disinfected with 0.1% mercuric chloride solution for 8 min or disinfected with 2% sodium hypochlorite solution for 12 min, the explant infection rate was the lowest;2) The optimal medium for primary culture of stem segments was MS + 6-BA 2.0 mg/L + NAA 0.05 mg/L, and the induction rate was89.30%; 3) The optimal proliferation medium was MS + 6-BA 2.0 mg/L + NAA 0.1 mg/L + KT 1.0 mg/L, the proliferation multiple was4.4, and the growth condition was good; 4) 1/2 MS + IBA 0.2 mg/L medium was the best rooting medium; 5) This study established a tissue culture regeneration system of A. triloba, which laid a foundation for planting seedlings and large-scale promotion.
引文
[1]徐毅励,陈媛梅.重瓣榆叶梅花中蛋白质提取工艺及动态含量研究[J].西北林学院学报,2015,30(3):191-196.
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[6]戴小英,刘新亮,邱凤英,等.黄樟茎段离体培养体系的建立[J].中南林业科技大学学报,2018,38(3):20-25,33.
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[10]李世敬,李殿波.重瓣榆叶梅的组织培养[J].中国林副特产,1992(3):40-41.
[11]杨宏,陈文静,杨标,等.长山核桃茎段组织培养初步研究[J].经济林研究,2017,35(2):220-224.
[12]张东旭,周增产,卜云龙,等.植物组织培养技术应用研究进展[J].北方园艺,2011(6):209-213.
[13]冼康华,付传明,唐凤鸾,等.文采唇柱苣苔的组织培养与快速繁殖[J].植物生理学报,2014(7):1065-1069.
[14]孟永红,李燕玲,杜克久.楸树植株再生体系的建立[J].河北林果研究,2004,19(2):101-104.
[15]THAKUR R,RAO P S, BAPAT V A. In vitro plant regeneration in Melia azedarach[J].Plant Cell Reports,1998,18:127-131.
[2]周鑫.重瓣榆叶梅微型繁殖技术[J].现代园艺,2011(8):16-17.
[3]李凤飞.鸾枝榆叶梅离体快繁技术的研究[D].吉林:吉林农业大学,2016.
[4]杜瑛,鲍艳杰.榆叶梅的繁育技术[J].阴山学刊(自然科学),2010,24(3):54-58.
[5]宋润刚,李昌禹,张亚风,等.重瓣榆叶梅组织培养与快速繁殖研究[J].北方园艺,1996(5):19-19.
[6]戴小英,刘新亮,邱凤英,等.黄樟茎段离体培养体系的建立[J].中南林业科技大学学报,2018,38(3):20-25,33.
[7]吴高殷,田有亮,何炎红,等.山杏茎尖和茎段组织培养体系的建立[J].经济林研究,2017,35(2):151-156.
[8]冷肖荀,王青华.重瓣榆叶梅茎尖培养[J].河北林业科技,2000(5):4.
[9]吴玲利,柯镔峰,龚春,等.白木通组织培养及快速繁殖[J].植物生理学报,2015(6):903-908.
[10]李世敬,李殿波.重瓣榆叶梅的组织培养[J].中国林副特产,1992(3):40-41.
[11]杨宏,陈文静,杨标,等.长山核桃茎段组织培养初步研究[J].经济林研究,2017,35(2):220-224.
[12]张东旭,周增产,卜云龙,等.植物组织培养技术应用研究进展[J].北方园艺,2011(6):209-213.
[13]冼康华,付传明,唐凤鸾,等.文采唇柱苣苔的组织培养与快速繁殖[J].植物生理学报,2014(7):1065-1069.
[14]孟永红,李燕玲,杜克久.楸树植株再生体系的建立[J].河北林果研究,2004,19(2):101-104.
[15]THAKUR R,RAO P S, BAPAT V A. In vitro plant regeneration in Melia azedarach[J].Plant Cell Reports,1998,18:127-131.