两种栀子挥发油的GC-MS成分分析
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摘要
采用水蒸气蒸馏法提取两个产地栀子中的挥发油,并通过气-质联用的方法测定其挥发性化学成分,色谱柱为DB-5MS弹性石英毛细管柱(30 m×0. 25 mm,0. 25μm),载气为氦气,流速1. 0 m L/min,进样口温度280℃,三阶程序升温,进样方式为分流进样,分流比20∶1,进样量1μL.从云南和河南样品中分别鉴定了27个和20个化合物的结构.用峰面积归一化法进行相对定量,质量分数超过5%的成分有N-甲基吡咯烷酮、乙酸丁酯、邻苯二甲酸二丁酯、己二酸二(2-乙基己基)酯等.
The essential oil from two Gardenia jasminoides Ellis was extracted by water distillation extraction method. The chemical constituents were separated and identified with GC-MS. Quartz capillary column DB-5 MS( 30 m × 0. 25 mm,0. 25 μm). The carrier gas was helium and the flow rate was 1. 0 m L/min. The temperature of injection port was 280℃. Temperature rises with three order programmed route. The injection method was split-flow and the ratio of split-flow was 20∶ 1. 1 μL sample was injected. The compounds were identified from Yunnan and Henan samples were 27 and 20 respectively. The relative contents of components were determined by area normalization method. The mass fractions exceed five percent include: 2-pyrrolidinone 1-methyl; acetic acid butyl ester; dibutyl phthalate and hexanedioic acid,bis( 2-ethylhexyl) ester.
The essential oil from two Gardenia jasminoides Ellis was extracted by water distillation extraction method. The chemical constituents were separated and identified with GC-MS. Quartz capillary column DB-5 MS( 30 m × 0. 25 mm,0. 25 μm). The carrier gas was helium and the flow rate was 1. 0 m L/min. The temperature of injection port was 280℃. Temperature rises with three order programmed route. The injection method was split-flow and the ratio of split-flow was 20∶ 1. 1 μL sample was injected. The compounds were identified from Yunnan and Henan samples were 27 and 20 respectively. The relative contents of components were determined by area normalization method. The mass fractions exceed five percent include: 2-pyrrolidinone 1-methyl; acetic acid butyl ester; dibutyl phthalate and hexanedioic acid,bis( 2-ethylhexyl) ester.
引文
[1]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药工业出版社,2015.
[2]于洋,高昊,戴毅,等.栀子属植物化学成分的研究进展[J].中草药,2010,41(1):148-153.
[3]陈红,肖永庆,李丽,等.栀子化学成分研究[J].中国中药杂志,2007,32(11):1041-1046.
[4]刘洁宇,张宏桂,周小平,等.中药栀子挥发油成分分析[J].白求恩医科大学学报,1999,25(1):25.
[5]刘慧,姚蓝,陈建红,等.栀子不同炮制品中挥发油类成分的GC-MS分析[J].中国中药杂志,2015,40(9):1732-1737.
[6]吉力,徐植灵,潘泪尤.栀子果实挥发油的GC-MS分析[J].中国药学杂志,1993,28(7):398-401.
[7]母多,徐兴梦,郭亚东,等.玫瑰精油的提取工艺及化学成分的GC-MS分析研究[J].昆明学院学报,2017,39(6):78-84.
[2]于洋,高昊,戴毅,等.栀子属植物化学成分的研究进展[J].中草药,2010,41(1):148-153.
[3]陈红,肖永庆,李丽,等.栀子化学成分研究[J].中国中药杂志,2007,32(11):1041-1046.
[4]刘洁宇,张宏桂,周小平,等.中药栀子挥发油成分分析[J].白求恩医科大学学报,1999,25(1):25.
[5]刘慧,姚蓝,陈建红,等.栀子不同炮制品中挥发油类成分的GC-MS分析[J].中国中药杂志,2015,40(9):1732-1737.
[6]吉力,徐植灵,潘泪尤.栀子果实挥发油的GC-MS分析[J].中国药学杂志,1993,28(7):398-401.
[7]母多,徐兴梦,郭亚东,等.玫瑰精油的提取工艺及化学成分的GC-MS分析研究[J].昆明学院学报,2017,39(6):78-84.