金蝉花多糖对小鼠免疫功能的影响
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摘要
为探讨金蝉花多糖对环磷酰胺(CTX)致免疫抑制小鼠的免疫调节作用,将70只试验小鼠随机分为7组,除正常组外,其他6组腹腔注射CTX以建立免疫低下模型,其中,高、中、低剂量组灌胃不同剂量的金蝉花多糖溶液30 d,正常对照组、CTX模型组给予等体积生理盐水,茯苓多糖组、盐酸左旋咪唑组分别给予等体积茯苓多糖溶液和盐酸左旋咪唑溶液,分别测定各组的小鼠体重增量、免疫器官指数、迟发型超敏反应、血清溶血素水平、IFN-γ、IL-2、IgG含量等指标。结果表明,与CTX模型组相比,金蝉花多糖中、高剂量组能显著提高小鼠脾脏指数、胸腺指数、血清溶血素水平(P<0.05);各剂量组均能显著影响小鼠迟发型超敏反应(P<0.05),均显著提高了IFN-γ、IL-2、IgG含量(P<0.05),说明金蝉花多糖对小鼠免疫功能具有一定的调节作用。本研究结果为进一步探究金蝉花多糖的免疫作用机制提供了参考,有利于金蝉花多糖在保健功效方面的应用研究。
To investigate the modulating effect of Cordyceps cicadae polysaccharides on the immunologic function of Mice.Seventy mice were randomly divided into seven groups,except for the normal group,the other groups were injected with cyclophosphamide(CTX) and immunosuppressed mouse models were established. The mice of high-dose,mid-dose group and low dose were lavage treatment with polysaccharides of Cordyceps cicadae for 30 days. The normal group and CXT model groups were gavaged with the equal volume physiological saline intraperitoneally,and the pachymaran group and levamisole hydrochloride group were given the equal volume pachymaran solution and levamisole hydrochloride solution solution,respectively. The weight,spleen index,thymus index,the level of IFN-γ,IL-2,Ig G,haemolysin and other biochemical indicators were measured,respectively. The results showed that mid-dose group and high-dose group significantly(P < 0. 05) improved spleen index,thymus index and haemolysin when compared to CTX model group. All of the dose groups significantly delayed hypersensitivity(P < 0. 05),and improved the content of IFN-γ,IL-2, Ig G(P < 0. 05). These results illustrated that polysaccharides of Cordyceps cicadae can regulate the immunologic function of mice. This study provides a new reference for further study on immune mechanism of polysaccharides of Cordyceps cicadae, which prmotes the research on the health efficacy of Cordyceps cicadae polysaccharides.
To investigate the modulating effect of Cordyceps cicadae polysaccharides on the immunologic function of Mice.Seventy mice were randomly divided into seven groups,except for the normal group,the other groups were injected with cyclophosphamide(CTX) and immunosuppressed mouse models were established. The mice of high-dose,mid-dose group and low dose were lavage treatment with polysaccharides of Cordyceps cicadae for 30 days. The normal group and CXT model groups were gavaged with the equal volume physiological saline intraperitoneally,and the pachymaran group and levamisole hydrochloride group were given the equal volume pachymaran solution and levamisole hydrochloride solution solution,respectively. The weight,spleen index,thymus index,the level of IFN-γ,IL-2,Ig G,haemolysin and other biochemical indicators were measured,respectively. The results showed that mid-dose group and high-dose group significantly(P < 0. 05) improved spleen index,thymus index and haemolysin when compared to CTX model group. All of the dose groups significantly delayed hypersensitivity(P < 0. 05),and improved the content of IFN-γ,IL-2, Ig G(P < 0. 05). These results illustrated that polysaccharides of Cordyceps cicadae can regulate the immunologic function of mice. This study provides a new reference for further study on immune mechanism of polysaccharides of Cordyceps cicadae, which prmotes the research on the health efficacy of Cordyceps cicadae polysaccharides.
引文
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[29]Gong Y,Wu J,Li S T.Immuno-enhancement effects of Lycium ruthenicum Murr polysaccharide on cyclophosphamide-induced immunosuppression in mice[J].International Journal of Clinical and Experimental Medicine,2015,8(11):20631-20637
[30]Raj S,Gothandam K M.Immunomodulatory activity of methanolic extract of amorphophallus commutatus varwayanadensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models[J].Food Chemical Toxicology,2015,81:151-159
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[36]Wang M,Meng X X,Yang R L,Qin T,Li Y,Zhang L F,Fei CZ,Zhen W L,Zhang K,Wang X,Hu Y,Xue F.Cordyceps militaris polysaccharides can improve the immune efficacy of Newcastle disease vaccine in chicken[J].International Journal of Biological Macromolecules,2013,59:178-183
[37]Huang X H,Chen Q X,Wang Q,Song K K,Wang J,Sha L,Guan X.Inhibition of the activity of mushroom tyrosinase by alkybenzoic acids[J].Food Chemistry,2006,94(1):1-6
[38]柯春林,乔德亮,曾晓雄.兽疫链球菌荚膜多糖对免疫抑制鼠的抗氧化和免疫调节活性研究[J].中国微生态学杂志,2011,23(8):684-688
[39]Jiang S,Qiu L M,Li Y Q,Li L,Wang X Y,Liu Z,Guo Y,Wang H T.Effects of Marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H22 tumor-bearing mice[J].Carbohydrate Polymers,2016,137(2):52-58
[40]Li W J,Li L,Zhen W Y,Wang L F,Pan M,Lv J Q,Wang F,Yao Y F,Nie S P,Xie M Y.Ganoderma atrum polysaccharide ameliorates ROS generation and apoptosis in spleen and thymus of immunosuppressed mice[J].Food and Chemical Toxicology,2017,90:199-208
[41]王君巧,聂少平,余强,殷鹏飞,高江宇,张翼良,谢明勇.黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用[J].食品科学,2012,23(33):274-277
[2]葛飞,夏成润,李春如,丁婷,邵颖,樊美珍.蝉拟青霉菌丝体与天然蝉花中化学成分的比较分析[J].菌物学报,2007,26(1):68-75
[3]于士军,柴新义,樊美珍.蝉花菌质主要营养成分和活性成分分析[J].食品与机械,2015,31(1):155-158
[4]Weng S C,Chou C J,Lin L C,Tsai W J,Kuo Y C.Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae[J].Journal of Ethnopharmacology,2002,83:79-85
[5]Kim H S,Kim J S,Lee H K,Ryu H S,Yeon J S,Kang J S,Hong J T,Kim Y,Han S B.Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4[J].Food and Chemical Toxicology,2012,50(9):3190-3197
[6]封燕.金蝉花多糖的结构特征及免疫活性初步研究[D].镇江:江苏大学,2016
[7]谢飞,李伟,陈美珍,余杰.野生蝉花多糖抗肿瘤活性及其作用机制[J].食品科学,2016,37(13):209-213
[8]黄小莉,朱培欣,胡远亮,葛向阳.蛹虫草多糖对急性酒精性肝损伤改善作用的研究[J].菌物学报,2017,26(2):242-250
[9]宋捷民,忻家础,朱英.蝉花对小鼠血糖及造血功能影响[J].中华中医药学刊,2007,25(6):1144-1145
[10]朱碧纯,柴一秋,章思思,彭秀秀,金轶伟,陈官菊.蝉花虫草活性成分的抗惊厥作用[J].菌物学报,2016,35(5):619-627
[11]Sharma S K,Gautam N,Atri N S.Optimized extraction,composition,antioxidant and antimicrobial activities of exo and intracellular polysaccharides from submerged culture of Cordyceps cicadae[J].BMC Complementary and Alternative Medicine,2015,15(1):446
[12]何晓波,芦柏震,周俐斐,陈峰阳,王春雷,蔡菊芬.蝉花总多糖对细胞免疫和体液免疫反应的促进作用[J].中华中医药学刊,2010,28(7):1465-1468
[13]Zhang J L,Yu Y C,Zhang Z F,Ding Y,Dai X G,Li Y.Effect of polysaccharide from cultured Cordyceps sinensis on immune function and anti-oxidation activity of mice exposed to60Co.[J].International Immunopharmacology,2011,11(12):2251-2257
[14]王米,胡元亮,孟新宇,杨锐乐,张丽芳,薛飞群.蛹虫草多糖对免疫抑制小鼠免疫功能的影响[J].中国畜牧兽医,2014(2):135-138
[15]Chen J,Zhu X Q,Yang L,Luo Y Y,Wang M Y,Liu X T,Liang KX,Gu X L.Effect of Glycyrrhiza uralensis Fisch polysaccharide on growth performance and immunologic function in mice in Ural City,Xinjiang[J].Asian Pacific Journal of Tropical Medicine,2016,9(11):1078-1083
[16]Hou R,Chen J,Yue C,Li X,Gao Z,Liu C,Lu Y,Wang D,Li H,Hu Y.Modification of lily polysaccharide by selenylation and the immune-enhancing activity[J].Carbohydrate Polymers,2016,142:73-81
[17]Wang M,Meng X Y,Yang R L,Qin T,Wang X Y,Zhang K Y,Fei C Z,Li Y I,Xue F Q.Cordyceps militaris polysaccharides can enhance the immunity and antioxidation activity in immunosuppressed mice[J].Carbohydrate Polymers,2012,89(2):461-466
[18]李增智,陈祝安,陈以平.国宝虫草金蝉花[M].安徽:合肥工业出版社,2014
[19]杨娜,王鸿飞,宋佳敏,沈晓俊,邵兴锋,许凤.超声波辅助提取裂褶菌多糖及分离纯化的研究[J].核农学报,2014,28(11):2015-2024
[20]孙晓丽,崔子寅,张明亮,孙长江,佟春玉,冯新,顾敬敏,雷连成,韩文瑜.枸杞多糖和茯苓多糖对免疫抑制小鼠免疫增强及对肠道黏膜的免疫调节作用[J].中国兽医学报,2015,35(3):450-455
[21]李芳,李八方,王景峰,张晶,李尧,赵雪.海带岩藻聚糖硫酸酯对小鼠的免疫调节作用[J].食品科学,2012,33(5):238-242
[22]李璐,黄宏业,韦现色,王秋华,杨善忠,何家康,杨剑,胡庭俊.不同剂量环磷酰胺对小鼠免疫功能抑制作用的研究[J].畜牧与饲料科学,2016,37(9):1-3
[23]Hernandes M R G,Moraes L C A,Ribeiro E B,Faqundes D L G,Honorio-Franca A C,Franca E L.In vitro immunomodulatory effects of microemulsions with levamisole delivery systems on blood phagocytes interacting with Giardia lamblia[J].Parasitology International,2017,66(3):299-304
[24]李自辉,张宁,董婉茹,于卉,刘树民.玄参多糖对正常生理小鼠和免疫低下状态小鼠免疫功能的影响[J].中国药房,2017,28(10):1316-1319
[25]卫生部卫生法制与监督局.保健食品检验与评价技术规范(2003年)[M].北京:人民卫生出版社,2003:27-29
[26]杨娜,王鸿飞,董栓全,许凤,李和生.裂褶菌多糖对小鼠免疫活性作用的研究[J].现代食品科技,2014,30(8):1-5
[27]Miyake M,Takit T,Hitomi S,Hakomori S.Correlation of expression of H/Le(y)/Le(b)antigens with survival in patients with carcinoma of the lung[J].New England Journal of Medicine,1996,327(1):14-18
[28]Chiu C H,Chyau C C,Chen C C,Lin C H,Cheng C H,Mong MC.Polysaccharide extract of Cordyceps sobolifera attenuates renal injury in endotoxemic rats[J].Food and Chemical Toxicology,2014,69:281-288
[29]Gong Y,Wu J,Li S T.Immuno-enhancement effects of Lycium ruthenicum Murr polysaccharide on cyclophosphamide-induced immunosuppression in mice[J].International Journal of Clinical and Experimental Medicine,2015,8(11):20631-20637
[30]Raj S,Gothandam K M.Immunomodulatory activity of methanolic extract of amorphophallus commutatus varwayanadensis under normal and cyclophosphamide induced immunosuppressive conditions in mice models[J].Food Chemical Toxicology,2015,81:151-159
[31]Yu G,Chen G,Huang B,Shao W,Zeng G.Effect of early enteral nultrition on postoperative nutritional status and immune function in elderly patients with esophageal cancer or cardiac cancer[J].Chinese Journal of Cancer,2013,25(3):299-305
[32]高文伟,王震,霍乃蕊,王丽廷,周芃来,乔天磊,郭泽恒,苏文婷.聚合草多肽的酶法制备及其免疫活性研究[J].核农学报,2017,31(7):1330-1338
[33]Cui H Y,Wang C L,Wang Y R,Li Z J,Chen M H,Li F J,Sun Y P.Pleurotus nebrodensis polysaccharide(PN-S)enhances the immunity of immunosuppressed mice[J].Chinese Journal of Natural Medicines,2015,13(10):760-766
[34]杨海莺,刘爱萍,蒋菁莉,张明,任发政.双歧杆菌体内免疫刺激功能研究[J].食品科学,2008,29(2):369-372
[35]Zheng Y,Zong Z M,Chen S L,Chen A H,Wei X Y.Ameliorative effect of Trametes orientalis polysaccharide against immunosuppression and oxidative stress in cyclophosphamde-treated mice[J].International Journal of Biological Macromolecules,2017,95:1216-1222
[36]Wang M,Meng X X,Yang R L,Qin T,Li Y,Zhang L F,Fei CZ,Zhen W L,Zhang K,Wang X,Hu Y,Xue F.Cordyceps militaris polysaccharides can improve the immune efficacy of Newcastle disease vaccine in chicken[J].International Journal of Biological Macromolecules,2013,59:178-183
[37]Huang X H,Chen Q X,Wang Q,Song K K,Wang J,Sha L,Guan X.Inhibition of the activity of mushroom tyrosinase by alkybenzoic acids[J].Food Chemistry,2006,94(1):1-6
[38]柯春林,乔德亮,曾晓雄.兽疫链球菌荚膜多糖对免疫抑制鼠的抗氧化和免疫调节活性研究[J].中国微生态学杂志,2011,23(8):684-688
[39]Jiang S,Qiu L M,Li Y Q,Li L,Wang X Y,Liu Z,Guo Y,Wang H T.Effects of Marsdenia tenacissima polysaccharide on the immune regulation and tumor growth in H22 tumor-bearing mice[J].Carbohydrate Polymers,2016,137(2):52-58
[40]Li W J,Li L,Zhen W Y,Wang L F,Pan M,Lv J Q,Wang F,Yao Y F,Nie S P,Xie M Y.Ganoderma atrum polysaccharide ameliorates ROS generation and apoptosis in spleen and thymus of immunosuppressed mice[J].Food and Chemical Toxicology,2017,90:199-208
[41]王君巧,聂少平,余强,殷鹏飞,高江宇,张翼良,谢明勇.黑灵芝多糖对免疫抑制小鼠的免疫调节和抗氧化作用[J].食品科学,2012,23(33):274-277