费菜不同组织总黄酮抗氧化与抑菌活性的研究
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摘要
本文采用费菜不同组织(嫩叶、老叶、茎、花)中总黄酮进行体外抗氧化能力及抑菌作用的研究。对Fe~(3+)还原活性、清除DPPH·、·OH进行体外抗氧化活性试验,并采用两倍稀释法探讨费菜不同组织抑制大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的活性。结果表明,费菜不同组织总黄酮的Fe~(3+)还原活性、DPPH·清除率、·OH清除率均显著高于L-抗坏血酸。其中,Fe~(3+)还原活性可达到810μmol TE/mL以上;对DPPH·以及·OH清除率的IC_(50)值最低分别为0. 8μg/mL(茎)和14μg/mL(花)。费菜各组织总黄酮均有一定的抑菌作用,对金黄色葡萄球菌的抑制效果较好;对于大肠杆菌,茎和嫩叶中的总黄酮的抑制效果明显比老叶和花强;而四个组织总黄酮对枯草芽孢杆菌的抑制作用相当,其中老叶总黄酮的抑制效果略好。该试验结果为费菜不同组织总黄酮的应用提供理论基础。
The antioxidant and antibacterial activities of total flavonoids from different parts of Sedum aizoon L were studied in this paper. Antioxidant activities of total flavonoids from tender leaves,old leaves,stems and flowers of Sedum aizoon L were evaluated by reducing power on Fe~(3+),DPPH and Hydroxyl Radical scavenging activity assays. Escherichia coli,Staphylococus aureus and Bacillus subtilis were used as experimental strains,the antibacterial activity of different parts were determined by the method of double dilution tests. The results showed that the Ferric Reducing Antioxidant Power,DPPH and Hydroxyl Radical scavenging activity of total flavonoids in different parts of Sedum aizoon L. were significantly higher than L-ascorbic acid. The Ferric Reducing antioxidant power of total flavonoids in different parts of Sedum aizoon L. could reach up to 810 μmol TE/mL and the IC_(50) values of DPPH free radical clearance and Hydroxyl Radical scavenging activities for stems and flowers were 0. 8 and 14 μg/mL,respectively. The total flavonoids in various parts of Sedum aizoon L. had certain antibacterial effect,and the inhibitory effect on Staphylococcus aureus was better.The antibacterial effect of total flavonoids in different parts had good inhibitory effect on Staphylococcus aureus;The antibacterial effect of total flavonoids in stems and tender leaves to Escherichia coli was significantly better than old leaves and flowers;While the antibacterial effect of total favonoids in the old leaves to Bacillus subtilis was a little better than other parts. The results can provide a scientific basis for the utilization of total flavonoids from different parts of Sedum aizoon L.
The antioxidant and antibacterial activities of total flavonoids from different parts of Sedum aizoon L were studied in this paper. Antioxidant activities of total flavonoids from tender leaves,old leaves,stems and flowers of Sedum aizoon L were evaluated by reducing power on Fe~(3+),DPPH and Hydroxyl Radical scavenging activity assays. Escherichia coli,Staphylococus aureus and Bacillus subtilis were used as experimental strains,the antibacterial activity of different parts were determined by the method of double dilution tests. The results showed that the Ferric Reducing Antioxidant Power,DPPH and Hydroxyl Radical scavenging activity of total flavonoids in different parts of Sedum aizoon L. were significantly higher than L-ascorbic acid. The Ferric Reducing antioxidant power of total flavonoids in different parts of Sedum aizoon L. could reach up to 810 μmol TE/mL and the IC_(50) values of DPPH free radical clearance and Hydroxyl Radical scavenging activities for stems and flowers were 0. 8 and 14 μg/mL,respectively. The total flavonoids in various parts of Sedum aizoon L. had certain antibacterial effect,and the inhibitory effect on Staphylococcus aureus was better.The antibacterial effect of total flavonoids in different parts had good inhibitory effect on Staphylococcus aureus;The antibacterial effect of total flavonoids in stems and tender leaves to Escherichia coli was significantly better than old leaves and flowers;While the antibacterial effect of total favonoids in the old leaves to Bacillus subtilis was a little better than other parts. The results can provide a scientific basis for the utilization of total flavonoids from different parts of Sedum aizoon L.
引文
1 Chen HZ(陈华珍),Chen JW(陈建伟). The content of total flavonoids in different organs and their TLC identification[J]. Chin Arch Tradit Chin Med(中华中医药学刊),2003,21:1867-1867.
2 Lin Z,Fang Y,Huang A,et al. Chemical constituents from Sedum aizoon and their hemostatic activity[J]. Pharm Biol,2014,52:1429-1434.
3 Qiang Y(强毅),Wang ZJ(王政军),Chen KK(陈克克),et al. Determination of total polyphenols and antimicrobial activity of methanol extract from Sedum aizoon L.[J]. Sci Technol Food Ind(食品工业科技),2013,34:53-56.
4 Wang QZ,Wang N,Chen ZX. Extraction and deproteinization of polysaccharides from Sedum aizoon L.[J]. Adv Mater Res,2014,864:460-464.
5 Xu HH(徐宏化),Cheng H(程慧),Wang ZJ(王正加),et al. The study of total polyphenols,total flavonoids and antioxidant capacity in pecan[Carya illinoinensis(Wangenh.)K.Koch] Kernerls[J]. J Nuclear Agric Sci(核农学报),2016,30:72-78.
6 Hu JW(胡居吾),Han XD(韩晓丹),Fu JP(付建平),et al. Antimicrobial and antioxidant effects of 3 chlorogenic acid extracts[J]. Nat Prod Res Dev(天然产物研究与开发),2017,29:1928-1933.
7 Xu F,Wang C,Wang H,et al. Antimicrobial action of flavonoids from Sedum aizoon L. against lactic acid bacteria in vitro,and in refrigerated fresh pork meat[J]. J Funct Foods,2018,40:744-750.
8 Wang W(王伟),Dai GM(代光明),Li SM(李书明),et al. Optimization of total flavonoid extraction from the invasive plant species Praxelis clematidea(Asteraceae)and its antibacterial activity[J]. Nat Prod Res Dev(天然产物研究与开发),2017,29:1343-1348.
9 Zhang M(张梅). Study on the dynamic variation of the flavonoids contents and the influences of the elicitor on the flavonoids contents and related enzyme activities in Rhododendron L.[D]. Fuzhou:Fujian Agriculture and Forestry University(福建农林大学),2012.
10 Wang HF(王鸿飞),Liu F(刘飞),Xu C(徐超),et al.Study on anti-microbial activity of total flavonoid and its different polar extracts from Sedum aizoon L.[J]. J Chin Instit Food Sci Technol(中国食品学报),2013,13:124-128.
11 Sun J,Liu RH. Apple phytochemical extracts inhibit proliferation of estrogen-dependent and estrogen-independent human breast cancer cells through cell cycle modulation[J]. J Agr and Food Chem,2008,56:11661-11667.
12 Dong DD(董迪迪),Wang HF(王鸿飞),Zhou ZQ(周增群),et al. Antioxidant activity and blood lipid regulation of Myrica rubra seed oil[J]. J Chin Cereals Oils Assoc(中国粮油学报),2014,29:53-57.
13 Tu ZC(涂宗财),Fu ZF(傅志丰),Wang H(王辉),et al.Comparison of antioxidant activities of various solvent extracts of sweet potato(Ipomoea batatas(L.)Lam.)leaves and identification of antioxidant constituents of the merthanol extract[J]. Food Sci(食品科学),2015,36:1-6.
14 Xie R(谢瑞),Wei YX(魏艳霞),Ding YZ(丁玉竹),et al.Effects of different processing methods on the antioxidant activity of polysaccharide from Nitraria sibirica pall[J]. Nat Prod Res Dev(天然产物研究与开发),2016,1:41-45.
15 Shen P(沈萍),Chen XD(陈向东). Experiment of microbiology(微生物学实验)[M]. Beijing:Higher Education Press,2007:28-34.
16 Zhang DM(张大明). Studies on the inhibition effects of gross extracts of Dioscorea zingiberensis Wright CH. on main crop fungus and bacteris[D]. Wuhan:Huazhong Agricultural University(华中农业大学),2005.
17 Xi LS(席利莎),Sun HN(孙红男),Mu TH(木泰华),et al. The antioxidant activity in vitro and processing stability of sweet potato leaf polyphenols[J]. J Chin Instit Food Sci Technol(中国食品学报),2015,15:147-156.
18 Huang JL,Wei CY,Li QH. Study on the content of forsythin,total flavonoids of Forsythia suspensa and their antioxidant activity[J]. Med Plant,2012,6:28-30.
19 Yue XJ(岳秀洁),Li C(李超),Fu X(扶雄),et al. Optimization of ultrasonic extraction of flavonoids from Moringa stenopetala leaves and their antioxidant activities[J]. Sci Technol Food Ind(食品工业科技),2016,37:226-231.
20 Weng YT(瓮岳太),Xue Y(薛煜),Xu S(徐硕),et al. Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro[J]. J Beijing Forestry Univ(北京林业大学学报),2017,39:38-47.
21 Ma SQ(马士巧),Ma HL(马海乐),Pan ZL(潘忠礼),et al. Ultrasound-assisted extraction of propolis’flavonoids from the northeast China and its antibacterial activity[J]. Food Ind(食品工业),2015,12:159-163.
2 Lin Z,Fang Y,Huang A,et al. Chemical constituents from Sedum aizoon and their hemostatic activity[J]. Pharm Biol,2014,52:1429-1434.
3 Qiang Y(强毅),Wang ZJ(王政军),Chen KK(陈克克),et al. Determination of total polyphenols and antimicrobial activity of methanol extract from Sedum aizoon L.[J]. Sci Technol Food Ind(食品工业科技),2013,34:53-56.
4 Wang QZ,Wang N,Chen ZX. Extraction and deproteinization of polysaccharides from Sedum aizoon L.[J]. Adv Mater Res,2014,864:460-464.
5 Xu HH(徐宏化),Cheng H(程慧),Wang ZJ(王正加),et al. The study of total polyphenols,total flavonoids and antioxidant capacity in pecan[Carya illinoinensis(Wangenh.)K.Koch] Kernerls[J]. J Nuclear Agric Sci(核农学报),2016,30:72-78.
6 Hu JW(胡居吾),Han XD(韩晓丹),Fu JP(付建平),et al. Antimicrobial and antioxidant effects of 3 chlorogenic acid extracts[J]. Nat Prod Res Dev(天然产物研究与开发),2017,29:1928-1933.
7 Xu F,Wang C,Wang H,et al. Antimicrobial action of flavonoids from Sedum aizoon L. against lactic acid bacteria in vitro,and in refrigerated fresh pork meat[J]. J Funct Foods,2018,40:744-750.
8 Wang W(王伟),Dai GM(代光明),Li SM(李书明),et al. Optimization of total flavonoid extraction from the invasive plant species Praxelis clematidea(Asteraceae)and its antibacterial activity[J]. Nat Prod Res Dev(天然产物研究与开发),2017,29:1343-1348.
9 Zhang M(张梅). Study on the dynamic variation of the flavonoids contents and the influences of the elicitor on the flavonoids contents and related enzyme activities in Rhododendron L.[D]. Fuzhou:Fujian Agriculture and Forestry University(福建农林大学),2012.
10 Wang HF(王鸿飞),Liu F(刘飞),Xu C(徐超),et al.Study on anti-microbial activity of total flavonoid and its different polar extracts from Sedum aizoon L.[J]. J Chin Instit Food Sci Technol(中国食品学报),2013,13:124-128.
11 Sun J,Liu RH. Apple phytochemical extracts inhibit proliferation of estrogen-dependent and estrogen-independent human breast cancer cells through cell cycle modulation[J]. J Agr and Food Chem,2008,56:11661-11667.
12 Dong DD(董迪迪),Wang HF(王鸿飞),Zhou ZQ(周增群),et al. Antioxidant activity and blood lipid regulation of Myrica rubra seed oil[J]. J Chin Cereals Oils Assoc(中国粮油学报),2014,29:53-57.
13 Tu ZC(涂宗财),Fu ZF(傅志丰),Wang H(王辉),et al.Comparison of antioxidant activities of various solvent extracts of sweet potato(Ipomoea batatas(L.)Lam.)leaves and identification of antioxidant constituents of the merthanol extract[J]. Food Sci(食品科学),2015,36:1-6.
14 Xie R(谢瑞),Wei YX(魏艳霞),Ding YZ(丁玉竹),et al.Effects of different processing methods on the antioxidant activity of polysaccharide from Nitraria sibirica pall[J]. Nat Prod Res Dev(天然产物研究与开发),2016,1:41-45.
15 Shen P(沈萍),Chen XD(陈向东). Experiment of microbiology(微生物学实验)[M]. Beijing:Higher Education Press,2007:28-34.
16 Zhang DM(张大明). Studies on the inhibition effects of gross extracts of Dioscorea zingiberensis Wright CH. on main crop fungus and bacteris[D]. Wuhan:Huazhong Agricultural University(华中农业大学),2005.
17 Xi LS(席利莎),Sun HN(孙红男),Mu TH(木泰华),et al. The antioxidant activity in vitro and processing stability of sweet potato leaf polyphenols[J]. J Chin Instit Food Sci Technol(中国食品学报),2015,15:147-156.
18 Huang JL,Wei CY,Li QH. Study on the content of forsythin,total flavonoids of Forsythia suspensa and their antioxidant activity[J]. Med Plant,2012,6:28-30.
19 Yue XJ(岳秀洁),Li C(李超),Fu X(扶雄),et al. Optimization of ultrasonic extraction of flavonoids from Moringa stenopetala leaves and their antioxidant activities[J]. Sci Technol Food Ind(食品工业科技),2016,37:226-231.
20 Weng YT(瓮岳太),Xue Y(薛煜),Xu S(徐硕),et al. Extraction of total flavonoids from Cronartium orientale aeciospores by response surface methodology and its antioxidant activity in vitro[J]. J Beijing Forestry Univ(北京林业大学学报),2017,39:38-47.
21 Ma SQ(马士巧),Ma HL(马海乐),Pan ZL(潘忠礼),et al. Ultrasound-assisted extraction of propolis’flavonoids from the northeast China and its antibacterial activity[J]. Food Ind(食品工业),2015,12:159-163.