北方汉族MICA基因遗传多态性及与HLA-B连锁不平衡研究
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摘要
目的了解MICA等位基因在中国北方汉族的分布及与HLA-B基因型的连锁关系。方法用直接测序法对北方汉族224个标本的HLA-B和MICA基因的2—6外显子进行测序。用Arlequin软件统计分析2个位点间的连锁关系。结果发现18个MICA的等位基因,其中MICA~*002(16.07%),MICA~*008(23.21%)和MICA~*010(21.65%)最为常见。MICA~*010-HLA-B~*46∶01,MICA~*045-HLA-B~*13∶01,MICA~*049-HLA-B~*51∶01有明显的连锁关系。结论北方汉族人群呈现特异MICA等位基因库,MICA与HLA-B具有连锁关系。
Objective To study MICA polymorphism and MICA-HLA-B linkage disequilibrium in Northern Han population. Methods The exons 2 to 6 of HLA-B and MICA gene in 224 samples of northern Han population were sequenced by PCR sequence-based-typing method. The linkage disequilibrium between the two loci was statistically analyzed by Arlequin software. Results 18 alleles of MICA were detected in 224 samples. The frequency of MICA~*002(16.07%),MICA~*008(23.21%)and MICA~*010(21.65%)were the most common in Northern Han population.There was linkage disequilibrium between MICA~*010-HLA-B~*46∶01, MICA~*045-HLA-B~*13∶01, MICA~*049-HLA-B~*51∶01. Conclusion There was a specific distribution of MICA allele in northern Han population, and there was a linkage disequilibrium between MICA and HLA-B.
Objective To study MICA polymorphism and MICA-HLA-B linkage disequilibrium in Northern Han population. Methods The exons 2 to 6 of HLA-B and MICA gene in 224 samples of northern Han population were sequenced by PCR sequence-based-typing method. The linkage disequilibrium between the two loci was statistically analyzed by Arlequin software. Results 18 alleles of MICA were detected in 224 samples. The frequency of MICA~*002(16.07%),MICA~*008(23.21%)and MICA~*010(21.65%)were the most common in Northern Han population.There was linkage disequilibrium between MICA~*010-HLA-B~*46∶01, MICA~*045-HLA-B~*13∶01, MICA~*049-HLA-B~*51∶01. Conclusion There was a specific distribution of MICA allele in northern Han population, and there was a linkage disequilibrium between MICA and HLA-B.
引文
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[10] Sohn YH,Cha CH,Oh HB,et al.MICA polymorphisms and haplotypes with HLA-B and HLA-DRB1 in Koreans.Tissue Antigens,2010,75(1):48-55.
[11] Wenda S,Faé I,Sanchez-Mazas A.et al.The distribution of MICA alleles in an Austrian population:evidence for increasing polymorphism.Human Immunology,2013,74(10):1295-1299.
[12] Wang WY,Tian W,Zhu FM,et al.MICA,MICB polymorphisms and linkage disequilibrium with HLA-B in a Chinese Mongolian Population.Scand J Immunol,2016,83(6):456-462.
[13] Tian W,Cai JH,Liu XX,et al.MICA genetic polymorphism and HLA-A,C,B,MICA and DRB1 haplotypic variation in a southern Chinese Han population:Identification of two new MICA alleles,MICA*060 and MICA*062.Human Immunology,2011,72 (6):510-515.
[2] Sumitran-Holgersson S.Relevance of MICA and other non-HLA antibodies in clinical transplantation.Curr Opin Immunol,2008,20(5):607-613.
[3] Muòoz-Sa? I,Cambra A,Pallarês L,et al.Allelic diversity and affinity variants of MICA are imbalanced in Spanish patients with Behèet′s disease.Scand J Immunol,2006,64(1);77-82.
[4] Zou Y,Stastny P.High resolution MICA genotyping by sequence-based typing (SBT).Methods Mol Biol,2012,882:183-195.
[5] 焦淑贤,迟晓云,杜占慧,等.山东地区汉族人群 MICA 基因多态性分析.临床检验杂志,2015,33(6):438-441.
[6] Zhu F,Zhao H,He Y,et al.Distribution of MICA diversity in the Chinese Han population by polymerase chain reaction sequence-based typing for exons 2-6.Tissue Antigens,2009,73(4):358-363.
[7] 徐秀章,叶欣,夏文杰,等.广东汉族人群MICA 基因多态性分析.中国输血杂志.2011,24(3) :259-260.
[8] 梅冰,霍治,杜昆,等.基因多态性分析.现代免疫学,2008,28(3) :233-237.
[9] Tian W,Cai JH,Wang F,et al.MICA polymorphism in a northern Chinese Han population:The identification of a new MICA allele,MICA*059.Human Immunology,2010,71(4):423-427.
[10] Sohn YH,Cha CH,Oh HB,et al.MICA polymorphisms and haplotypes with HLA-B and HLA-DRB1 in Koreans.Tissue Antigens,2010,75(1):48-55.
[11] Wenda S,Faé I,Sanchez-Mazas A.et al.The distribution of MICA alleles in an Austrian population:evidence for increasing polymorphism.Human Immunology,2013,74(10):1295-1299.
[12] Wang WY,Tian W,Zhu FM,et al.MICA,MICB polymorphisms and linkage disequilibrium with HLA-B in a Chinese Mongolian Population.Scand J Immunol,2016,83(6):456-462.
[13] Tian W,Cai JH,Liu XX,et al.MICA genetic polymorphism and HLA-A,C,B,MICA and DRB1 haplotypic variation in a southern Chinese Han population:Identification of two new MICA alleles,MICA*060 and MICA*062.Human Immunology,2011,72 (6):510-515.