两株植物乳杆菌作为口腔益生菌的特性
|
摘要
利用实验室前期筛选的两株具有优良抗氧化性能的植物乳杆菌AR113和AR501,研究其在口腔应用方面的潜在特性。对AR113和AR501的自聚集能力、与口腔细菌共聚集能力以及对口腔微环境中溶菌酶和盐的耐受性及其耐药性能进行检测。结果表明,AR113和AR501的自聚力在16 h能达到90%以上,并具有与口腔细菌Streptococcusoralis共聚集的能力,可耐受较高浓度的溶菌酶和盐溶液;对红霉素、氯霉素、氨苄西林敏感,对万古霉素、诺氟沙星和卡那霉素具有抗性。两株植物乳杆菌均表现出一定的口腔益生特性,具有作为口腔益生菌的应用潜力。
Lactobacillus plantarum AR113 and AR501 with antioxidant properties were used to study their oral application characteristics. The self-aggregation ability and co-aggregation with Streptococcus oralisof AR113 and AR501 were measured and the tolerance of lysozyme,salt and antibiotics were determined. The results showed that the self-polymerization of AR113 and AR501 reached more than 90% at 16 h and had the ability to coaggregate with oral bacteria. In addition,both these two Lactobacillus plantarum showed resistance up to 0.6 mg/mL lysozyme and a certain concentration of salt solution, which is beneficial to their survival in the oral environment. Moreover,they had sensitivity to erythromycin, chloramphenicol and ampicillin but resistance to vancomycin,norfloxacin and kanamycin. Oral probiotics properties of L.plantarum AR113 and AR501 provided the potential for their application in oral system.
Lactobacillus plantarum AR113 and AR501 with antioxidant properties were used to study their oral application characteristics. The self-aggregation ability and co-aggregation with Streptococcus oralisof AR113 and AR501 were measured and the tolerance of lysozyme,salt and antibiotics were determined. The results showed that the self-polymerization of AR113 and AR501 reached more than 90% at 16 h and had the ability to coaggregate with oral bacteria. In addition,both these two Lactobacillus plantarum showed resistance up to 0.6 mg/mL lysozyme and a certain concentration of salt solution, which is beneficial to their survival in the oral environment. Moreover,they had sensitivity to erythromycin, chloramphenicol and ampicillin but resistance to vancomycin,norfloxacin and kanamycin. Oral probiotics properties of L.plantarum AR113 and AR501 provided the potential for their application in oral system.
引文
[1]GAO L,XU T,HUANG G,et al.Oral microbiomes:more and more importance in oral cavity and whole body[J].Protein&Cell,2018,9(5):488-500.
[2]XIAO C,RAN S,HUANG Z,et al.Bacterial diversity and community structure of supragingival plaques in adults with dental health or caries revealed by 16Spyrosequencing[J].Frontiers in Microbiology,2016,7(119):1145.
[3]LIU B,FALLER L L,KLITGORD N,et al.Deep sequencing of the oral microbiome reveals signatures of periodontal disease[J].PloS One,2012,7(6):37919.
[4]COSTALONGA M,HERZBERG M C.The oral microbiome and the immunobiology of periodontal disease and caries[J].Immunology Letters,2014,162(2):22-38.
[5]ATARASHI K,SUDA W,LUO C,et al.Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammation[J].Science,2017,358(6361):359.
[6]JIE H,SHUWEN H,YAN C,et al.Variations of tongue coating microbiota in patients with gastric cancer[J].BioMed Research International,2015,2015(2):173729.
[7]HAN S,CHEN Y,HU J,et al.Tongue images and tongue coating microbiome in patients with colorectal cancer[J].Microbial Pathogenesis,2014(77):1-6.
[8]CHHIBBERGOEL J,SINGHAL V,BHOWMIK D,et al.Linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients[J].Npj Biofilms&Microbiomes,2016,2(1):7.
[9]BOADEN E,LYONS M,SINGHRAO S K,et al.Oral flora in acute stroke patients:A prospective exploratory observational study[J].Gerodontology,2017:1-14.
[10]OHSHIMA T,KOJIMA Y,SENEVIRATNE C J,et al.Therapeutic application of synbiotics,a fusion of probiotics and prebiotics,and biogenics as a new concept for oralcandidainfections:A mini review[J].Frontiers in Microbiology,2016(7):10.
[11]马千里,刘冬,顾瑞霞.植物乳杆菌的益生特性及其在乳制品中的应用[J].中国奶牛,2014(1):36-40.
[12]WASSENAAR TM,KLEIN G.Safety aspects and implications of regulation of probiotic bacteria in food and food supplements[J].Journal of Food Protection,2008,71(8):1734-1741.
[13]LIN X,XIA Y,WANG G,et al.Lactic acid bacteria with antioxidant activities alleviating oxidized oil induced hepatic injury in mice[J].Frontiers in Microbiology,2018(9):2684.
[14]LIN X,XIA Y,WANG G,et al.Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D-Galactose-Induced Aging Mice Liver by Upregulation of Nrf2-Mediated Antioxidant Enzyme Expression[J].Journal of Food Science,2018(83):1990-1998.
[15]杨结彬,郭兴华.乳酸菌:生物学基础及应用[M].北京:中国轻工业出版社,1996:1-30.
[16]GIL NF,MARTINEZ RC,GOMES BC,et al.Vaginal lactobacilli as potential probiotics against Candida SPP[J].Brazilian Journal of Microbiology,2010,41(1):6-14.
[17]金丹.口腔溃疡患者唾液溶菌酶水平的研究[J].湖北中医药大学学报,2009,11(2):26-27.
[18]郭夏蕾,张健,杨贞耐.植物乳杆菌耐受口腔不良环境及其抑菌特性研究[J].食品工业科技,2017,38(2):215-220.
[19]杨娟,堵国成,陈坚,等.口腔乳酸杆菌的分离及其益生特性[J].微生物学报,2013,53(4):403-408.
[20]ARGYRIAA,ZOUMPOPOULOU G,KARATZASKAG,et al.Selection of potential probiotic lactic acid bacteria from fermented olives by invitro tests[J].Food Microbiology,2013,33(2):282-291.
[2]XIAO C,RAN S,HUANG Z,et al.Bacterial diversity and community structure of supragingival plaques in adults with dental health or caries revealed by 16Spyrosequencing[J].Frontiers in Microbiology,2016,7(119):1145.
[3]LIU B,FALLER L L,KLITGORD N,et al.Deep sequencing of the oral microbiome reveals signatures of periodontal disease[J].PloS One,2012,7(6):37919.
[4]COSTALONGA M,HERZBERG M C.The oral microbiome and the immunobiology of periodontal disease and caries[J].Immunology Letters,2014,162(2):22-38.
[5]ATARASHI K,SUDA W,LUO C,et al.Ectopic colonization of oral bacteria in the intestine drives TH1 cell induction and inflammation[J].Science,2017,358(6361):359.
[6]JIE H,SHUWEN H,YAN C,et al.Variations of tongue coating microbiota in patients with gastric cancer[J].BioMed Research International,2015,2015(2):173729.
[7]HAN S,CHEN Y,HU J,et al.Tongue images and tongue coating microbiome in patients with colorectal cancer[J].Microbial Pathogenesis,2014(77):1-6.
[8]CHHIBBERGOEL J,SINGHAL V,BHOWMIK D,et al.Linkages between oral commensal bacteria and atherosclerotic plaques in coronary artery disease patients[J].Npj Biofilms&Microbiomes,2016,2(1):7.
[9]BOADEN E,LYONS M,SINGHRAO S K,et al.Oral flora in acute stroke patients:A prospective exploratory observational study[J].Gerodontology,2017:1-14.
[10]OHSHIMA T,KOJIMA Y,SENEVIRATNE C J,et al.Therapeutic application of synbiotics,a fusion of probiotics and prebiotics,and biogenics as a new concept for oralcandidainfections:A mini review[J].Frontiers in Microbiology,2016(7):10.
[11]马千里,刘冬,顾瑞霞.植物乳杆菌的益生特性及其在乳制品中的应用[J].中国奶牛,2014(1):36-40.
[12]WASSENAAR TM,KLEIN G.Safety aspects and implications of regulation of probiotic bacteria in food and food supplements[J].Journal of Food Protection,2008,71(8):1734-1741.
[13]LIN X,XIA Y,WANG G,et al.Lactic acid bacteria with antioxidant activities alleviating oxidized oil induced hepatic injury in mice[J].Frontiers in Microbiology,2018(9):2684.
[14]LIN X,XIA Y,WANG G,et al.Lactobacillus plantarum AR501 Alleviates the Oxidative Stress of D-Galactose-Induced Aging Mice Liver by Upregulation of Nrf2-Mediated Antioxidant Enzyme Expression[J].Journal of Food Science,2018(83):1990-1998.
[15]杨结彬,郭兴华.乳酸菌:生物学基础及应用[M].北京:中国轻工业出版社,1996:1-30.
[16]GIL NF,MARTINEZ RC,GOMES BC,et al.Vaginal lactobacilli as potential probiotics against Candida SPP[J].Brazilian Journal of Microbiology,2010,41(1):6-14.
[17]金丹.口腔溃疡患者唾液溶菌酶水平的研究[J].湖北中医药大学学报,2009,11(2):26-27.
[18]郭夏蕾,张健,杨贞耐.植物乳杆菌耐受口腔不良环境及其抑菌特性研究[J].食品工业科技,2017,38(2):215-220.
[19]杨娟,堵国成,陈坚,等.口腔乳酸杆菌的分离及其益生特性[J].微生物学报,2013,53(4):403-408.
[20]ARGYRIAA,ZOUMPOPOULOU G,KARATZASKAG,et al.Selection of potential probiotic lactic acid bacteria from fermented olives by invitro tests[J].Food Microbiology,2013,33(2):282-291.