顶空固相微萃取气质联用法分析保心宁片中挥发性成分
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摘要
研究保心宁片中挥发性成分,建立快速分析保心宁片中挥发性成分的方法。采用顶空固相微萃取法提取保心宁片的挥发性成分,并用气相色谱-质谱联用技术对其成分进行鉴定,通过色谱峰面积归一化法计算保心宁片中各挥发性成分的相对百分含量。结果,共鉴定了36种化学成分,主要为酮类、醛类、酚类等化合物。本实验从挥发性成分的角度深入了解保心宁片的药效物质基础研究,为其临床应用提供了科学依据。
The composition volatile constituents in Baoxinning tablets were investigated and a rapid method for the analysis of volatile constituents in Baoxinning tablets was established. The volatile constituents of Baoxinning tablets were extracted by headspace solid-phase micro-extraction method. The chemical composition of volatile constituents of Baoxinning tablets were identified by gas chromatographymass spectrometry. The relative content of constituents was determined by area normalization method. Thirty-six compounds were identified. The main volatile constituents of Baoxinning tablets were ketones, aldehydes and phenols.This study revealed the pharmacodynamic basis of Baoxining tablets from the perspective of volatile constituents. It provides scientific basis for its clinical application.
The composition volatile constituents in Baoxinning tablets were investigated and a rapid method for the analysis of volatile constituents in Baoxinning tablets was established. The volatile constituents of Baoxinning tablets were extracted by headspace solid-phase micro-extraction method. The chemical composition of volatile constituents of Baoxinning tablets were identified by gas chromatographymass spectrometry. The relative content of constituents was determined by area normalization method. Thirty-six compounds were identified. The main volatile constituents of Baoxinning tablets were ketones, aldehydes and phenols.This study revealed the pharmacodynamic basis of Baoxining tablets from the perspective of volatile constituents. It provides scientific basis for its clinical application.
引文
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[2]陈晓莉,陈建宗,吕召学.保心宁片治疗冠心病心绞痛的临床研究[J].广东药学院学报,2002,(3):233-236.
[3]叶欣,卢金清,曹利.顶空-固相微萃取技术在中药领域的运用[J].中国药房,2017,(6):861-864.
[4]魏丹,李祖光,徐心怡,等.HS-SPME-GC-MS联用分析3种兰花鲜花的香气成分[J].食品科学,2013,34(16):234-237.
[5]刘宝峰,刘平平,谢文兵.固相萃取-气相色谱-质谱法测定酱油中3-氯-1,2-丙二醇[J].分析化学,2011,(1):125-128.
[6]张晓珊,高文华,徐严平,等.顶空固相微萃取-GC-MS法分析藿香正气丸挥发性成分[J].汕头大学学报:自然科学版,2008,(2):50-55.
[7]周吴萍,甄汉深.冠心丹参片中降香挥发油鉴别和成分分析[J].广西工学院学报,2007,(2):89-91.
[8]刘宝峰,高丰展,房强,等.间接顶空固相微萃取-气相色谱-质谱法对红花香雪兰天然花香成分的分析[J].分析化学,2016,(3):444-450.
[9]典灵辉,龚先玲,张立坚,等.丹参挥发油成分的分析[J].时珍国医国药,2006,(1):34-35.
[10]梁卉,刘绍华,欧阳坚,等.枳壳挥发油化学成分及其抑菌活性分析[J].安徽农业科学,2017,(11):111-113.
[11]施丽娜,刘润民,曹树明,等.市售三七挥发油成分的研究[J].昆明医学院学报,1989,(4):6-8.
[12]张小飞,詹娟娟,吴司琪,等.当归挥发油提取工艺优化及其乳化芳香水成分分析[J].中国实验方剂学杂志,2017,(5):27-31.